FastQCFastQC Report
Thu 2 Feb 2017
SRR4063930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863988
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9150.029.8131891
GGTATCA3700.026.1612681
GTATAAA1202.3646862E-1123.8325181
TTGCGCG550.004484983619.99815818
TATCAAC13600.019.7334772
GTATATC901.0017129E-519.5548881
TCAACGC14150.018.967554
GTACTAA708.1226305E-418.8564971
ATCAACG14350.018.7031943
GTCTAGC600.007411896318.3327061
ATAAGAC1203.1605123E-718.3327063
GTATAGG1203.1605123E-718.3327061
CAACGCA14800.018.1345165
AACGCAG15250.017.7436546
ATACCGT953.3458025E-416.2099726
TACCGTG850.002978549615.529787
GTACCGA850.002979698615.5288826
CTATAGT850.002979698615.5288824
ACCGATC1004.9407734E-415.4003658
TATATCT1004.9451215E-415.3985822