FastQCFastQC Report
Thu 2 Feb 2017
SRR4063929_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063929_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859106
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32860.3824906356142315No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25610.2981005836299595No Hit
TATCAACGCAGAGTACTTTTTTTTT23340.2716777673535047No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17300.2013721240452284No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13350.15539409572276297No Hit
GGTATCAACGCAGAGTACTTTTTTT10950.12745807851417637No Hit
GTCCTACAGTGGACATTTCTAAATT9300.10825206668327307No Hit
GTCCTAAAGTGTGTATTTCTCATTT9260.1077864663964633No Hit
CTGTAGGACGTGGAATATGGCAAGA9160.10662246567943885No Hit
GTACATGGGGTGGTATCAACGCAAA8800.10243206309815085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGT401.2836394E-519.0008148
GCGTTAT350.00217273716.281671
TCGAACT551.1248107E-515.548840519
CGAGGTT602.5730478E-514.2456314
GCACGCC707.2371913E-613.57438415
TAGCGTA707.2502717E-613.5720117
GACCGAG500.001498109413.30057057
ACTGTTC1700.012.8534948
AGCGTAT905.3927033E-712.6672118
CTAAACA853.951025E-612.290354
CGCATCG701.0881824E-412.21623313
ACAGTCC1103.8022336E-812.0914288
GAACGTC550.003070890412.0886125
TAGGCAG550.003070890412.0886125
CGTTATT550.003077595512.0850922
ACGGTAT1353.6925485E-1011.9634769
ACGCCCC802.8564862E-511.87758617
CCGACCA1209.978976E-911.8755099
ATAGCGT802.8667246E-511.8734346
AAGACGG1459.640644E-1111.7908625
Produced by FastQC (version 0.11.5)