##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063929_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	859106
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172317502147582	32.0	32.0	32.0	32.0	32.0
2	30.897769309025893	32.0	32.0	32.0	32.0	32.0
3	30.905788109965474	32.0	32.0	32.0	32.0	32.0
4	30.901336971223575	32.0	32.0	32.0	32.0	32.0
5	30.898972885767297	32.0	32.0	32.0	32.0	32.0
6	34.394056146738585	36.0	36.0	36.0	32.0	36.0
7	34.338832460720795	36.0	36.0	36.0	32.0	36.0
8	34.29861972794975	36.0	36.0	36.0	32.0	36.0
9	34.408813347829025	36.0	36.0	36.0	32.0	36.0
10	34.17169126976182	36.0	36.0	36.0	32.0	36.0
11	34.403730156697776	36.0	36.0	36.0	32.0	36.0
12	34.2572988664961	36.0	36.0	36.0	32.0	36.0
13	34.33399952974371	36.0	36.0	36.0	32.0	36.0
14	34.24006932788271	36.0	36.0	36.0	32.0	36.0
15	34.18346862901667	36.0	36.0	36.0	32.0	36.0
16	34.196014228744765	36.0	36.0	36.0	32.0	36.0
17	34.122598375520596	36.0	36.0	36.0	32.0	36.0
18	34.118215912821	36.0	36.0	36.0	32.0	36.0
19	34.1047460965236	36.0	36.0	36.0	32.0	36.0
20	34.068037005910796	36.0	36.0	36.0	32.0	36.0
21	34.02502135941316	36.0	36.0	36.0	32.0	36.0
22	34.01808973514328	36.0	36.0	36.0	32.0	36.0
23	33.970347081733806	36.0	36.0	36.0	32.0	36.0
24	33.953533091376386	36.0	36.0	36.0	32.0	36.0
25	33.35884396104788	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	5.0
5	21.0
6	36.0
7	18.0
8	51.0
9	55.0
10	80.0
11	29.0
12	40.0
13	48.0
14	119.0
15	299.0
16	462.0
17	682.0
18	874.0
19	1019.0
20	1291.0
21	1696.0
22	2284.0
23	2975.0
24	4188.0
25	5679.0
26	7954.0
27	10806.0
28	14897.0
29	20754.0
30	28982.0
31	42635.0
32	65114.0
33	99316.0
34	216162.0
35	330532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.11638034930989	17.49856482284732	11.563277630216854	25.821777197625934
2	15.94786442982395	19.842433893475256	38.956054701141234	25.25364697555956
3	19.118025363900383	24.353302021313127	28.27440504251567	28.25426757227082
4	12.301762832451763	15.40546077627952	36.373668330477535	35.919108060791174
5	14.054820481896854	37.02838809716166	33.90180926681738	15.014982154124109
6	33.537607569943745	35.69680642998175	17.33110121819469	13.434484781879819
7	29.705729970136396	30.478807318620802	21.173121590862785	18.642341120380017
8	27.427958596934943	33.82286670462763	19.65539365865644	19.093781039780993
9	27.295595433440045	14.388064429348871	18.98688331002228	39.3294568271888
10	15.584724147168098	26.908724566450427	32.45000640570224	25.056544880679237
11	36.61924261270201	21.103820478060552	23.28239207300882	18.99454483622862
12	24.938820342046995	23.587451676514814	29.72195723655474	21.751770744883448
13	29.602446981865448	19.94777100778426	25.61468655033563	24.835095460014653
14	22.694340712716567	20.399921725578263	25.992582566702154	30.913154995003016
15	24.185042139739007	28.527485173054796	23.080417250970214	24.207055436235983
16	24.392871827785147	25.95010652188853	25.757912846574698	23.89910880375162
17	22.863628898592623	26.424528884266092	26.44339797355299	24.268444243588295
18	23.60959994035568	25.072400287970254	28.32394789509123	22.994051876582848
19	24.94524952238945	25.05335259307581	26.365150738548998	23.63624714598574
20	25.24903629854766	24.68660044337839	26.37076265509798	23.69360060297597
21	25.563223103912623	24.401830271232406	25.497173982936726	24.53777264191824
22	25.13454952121339	25.318958085785514	25.907597679457606	23.638894713543486
23	23.426283402960454	25.24272410185675	26.72143637681126	24.609556118371543
24	24.20157122953837	25.269210517732247	26.78778615994669	23.741432092782688
25	23.914307182423418	25.369663857225234	26.695479459235916	24.02054950111543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158.0
1	158.0
2	404.0
3	650.0
4	650.0
5	650.0
6	1920.5
7	3191.0
8	3191.0
9	3191.0
10	3087.0
11	2983.0
12	2983.0
13	2983.0
14	2854.5
15	2726.0
16	2726.0
17	2726.0
18	4703.5
19	6681.0
20	6681.0
21	6681.0
22	10867.0
23	15053.0
24	15053.0
25	15053.0
26	22665.0
27	30277.0
28	30277.0
29	30277.0
30	38425.0
31	46573.0
32	46573.0
33	46573.0
34	53643.0
35	60713.0
36	60713.0
37	60713.0
38	67760.0
39	74807.0
40	74807.0
41	74807.0
42	82983.0
43	91159.0
44	91159.0
45	91159.0
46	101278.0
47	111397.0
48	111397.0
49	111397.0
50	111236.0
51	111075.0
52	111075.0
53	111075.0
54	101521.0
55	91967.0
56	91967.0
57	91967.0
58	84135.0
59	76303.0
60	76303.0
61	76303.0
62	66595.0
63	56887.0
64	56887.0
65	56887.0
66	46892.0
67	36897.0
68	36897.0
69	36897.0
70	27932.0
71	18967.0
72	18967.0
73	18967.0
74	14475.5
75	9984.0
76	9984.0
77	9984.0
78	8111.5
79	6239.0
80	6239.0
81	6239.0
82	4241.0
83	2243.0
84	2243.0
85	2243.0
86	1724.5
87	1206.0
88	1206.0
89	1206.0
90	809.5
91	413.0
92	413.0
93	413.0
94	262.5
95	112.0
96	112.0
97	112.0
98	278.5
99	445.0
100	445.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03806282344669924
2	0.004423202724692879
3	0.001280400788726886
4	0.0039576024378831015
5	0.00977760602300531
6	0.015132009321317742
7	0.02339641441219128
8	0.03829562359010413
9	0.04993563076034855
10	0.05773443556441231
11	0.05470803370014876
12	0.06623164079869073
13	0.06739564151571519
14	0.06844324216103717
15	0.062157638289105185
16	0.06949084280635917
17	0.0650676400816663
18	0.07961764904447181
19	0.07752244775382781
20	0.0811308499766036
21	0.07612564689339849
22	0.0798504491878767
23	0.08427365191256958
24	0.0779880480406376
25	0.08043244954638892
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	859106.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.66472565343314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.38268596336655	46.56963493673537
2	13.103242116129646	15.373962078265127
3	3.638208458215428	6.403035032136241
4	1.4264588539630694	3.3473126929461645
5	0.7166571994254746	2.1021248995926594
6	0.3796386289621513	1.336283760930606
7	0.24681667513642688	1.0135602773499712
8	0.14994696542871364	0.7037278071552248
9	0.12401544168027966	0.6547798676666647
>10	0.6718618848156107	7.44828787493943
>50	0.08222633375080042	3.420888144039776
>100	0.07206975941484657	8.233645816607881
>500	0.004977193315376525	1.9513986990532293
>1k	0.001194526395690366	1.4413581125817514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3286	0.3824906356142315	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2561	0.2981005836299595	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2334	0.2716777673535047	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1730	0.2013721240452284	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1335	0.15539409572276297	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1095	0.12745807851417637	No Hit
GTCCTACAGTGGACATTTCTAAATT	930	0.10825206668327307	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	926	0.1077864663964633	No Hit
CTGTAGGACGTGGAATATGGCAAGA	916	0.10662246567943885	No Hit
GTACATGGGGTGGTATCAACGCAAA	880	0.10243206309815085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1640007170244416E-4
2	0.0	0.0	0.0	0.0	1.1640007170244416E-4
3	0.0	0.0	0.0	0.0	1.1640007170244416E-4
4	0.0	0.0	0.0	0.0	1.1640007170244416E-4
5	0.0	0.0	0.0	0.0	1.1640007170244416E-4
6	0.0	0.0	0.0	0.0	1.1640007170244416E-4
7	0.0	0.0	0.0	0.0	1.1640007170244416E-4
8	0.0	0.0	0.0	0.0	1.1640007170244416E-4
9	0.0	0.0	0.0	0.0	1.1640007170244416E-4
10	0.0	0.0	0.0	0.0	1.1640007170244416E-4
11	0.0	0.0	0.0	0.0	1.1640007170244416E-4
12	0.0	0.0	0.0	0.0	5.820003585122208E-4
13	0.0	0.0	0.0	0.0	8.148005019171092E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGT	40	1.2836394E-5	19.000814	8
GCGTTAT	35	0.002172737	16.28167	1
TCGAACT	55	1.1248107E-5	15.5488405	19
CGAGGTT	60	2.5730478E-5	14.245631	4
GCACGCC	70	7.2371913E-6	13.574384	15
TAGCGTA	70	7.2502717E-6	13.572011	7
GACCGAG	50	0.0014981094	13.3005705	7
ACTGTTC	170	0.0	12.853494	8
AGCGTAT	90	5.3927033E-7	12.667211	8
CTAAACA	85	3.951025E-6	12.29035	4
CGCATCG	70	1.0881824E-4	12.216233	13
ACAGTCC	110	3.8022336E-8	12.091428	8
GAACGTC	55	0.0030708904	12.088612	5
TAGGCAG	55	0.0030708904	12.088612	5
CGTTATT	55	0.0030775955	12.085092	2
ACGGTAT	135	3.6925485E-10	11.963476	9
ACGCCCC	80	2.8564862E-5	11.877586	17
CCGACCA	120	9.978976E-9	11.875509	9
ATAGCGT	80	2.8667246E-5	11.873434	6
AAGACGG	145	9.640644E-11	11.790862	5
>>END_MODULE