FastQCFastQC Report
Thu 2 Feb 2017
SRR4063929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859106
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9330.1086012668983804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG300.00574577229.33158512
TGTAGGA7700.021.1415982
CTGTAGG7300.020.495511
GTAGGAC7550.019.2306443
GGCGTTA2100.018.85711942
GATATAC1801.4551915E-1118.3354431
CGCGGGA1701.2551027E-1018.11762244
GTCCTAT2200.018.0020711
TTCGTAT2600.017.76920725
TAGGACG6950.017.7255634
TAGGACC5750.017.5989534
TCTATAG1002.482927E-517.5989533
GACGTGA4150.017.4929357
CGCAATA2150.017.39633836
CGGCGTT2300.017.2173741
ATTTCGT3200.017.18747742
GGACGTG7350.017.060216
GTCCTAC6450.017.0562271
TAGCGGC2201.8189894E-1217.00195730
AGCGAAA2201.8189894E-1216.99997717
Produced by FastQC (version 0.11.5)