##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4063929_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 859106 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.131196848817257 32.0 32.0 32.0 32.0 32.0 2 31.112497177298263 32.0 32.0 32.0 32.0 32.0 3 31.300286576976532 32.0 32.0 32.0 32.0 32.0 4 31.43394877931245 32.0 32.0 32.0 32.0 32.0 5 31.322933374926958 32.0 32.0 32.0 32.0 32.0 6 34.905381873715235 36.0 36.0 36.0 36.0 36.0 7 34.933594923094475 36.0 36.0 36.0 36.0 36.0 8 34.85743552018028 36.0 36.0 36.0 32.0 36.0 9 35.03156420744355 36.0 36.0 36.0 36.0 36.0 10 34.82094293370085 36.0 36.0 36.0 32.0 36.0 11 35.032940056291075 36.0 36.0 36.0 36.0 36.0 12 34.9174129851264 36.0 36.0 36.0 32.0 36.0 13 34.97016782562338 36.0 36.0 36.0 36.0 36.0 14 34.90687645063589 36.0 36.0 36.0 32.0 36.0 15 34.8473040579393 36.0 36.0 36.0 32.0 36.0 16 34.87990539002172 36.0 36.0 36.0 32.0 36.0 17 34.83864505660536 36.0 36.0 36.0 32.0 36.0 18 34.846550949475386 36.0 36.0 36.0 32.0 36.0 19 34.833465253414595 36.0 36.0 36.0 32.0 36.0 20 34.82283094286386 36.0 36.0 36.0 32.0 36.0 21 34.810068839002405 36.0 36.0 36.0 32.0 36.0 22 34.7851464196502 36.0 36.0 36.0 32.0 36.0 23 34.75271270367102 36.0 36.0 36.0 32.0 36.0 24 34.7116805143952 36.0 36.0 36.0 32.0 36.0 25 34.669891724653304 36.0 36.0 36.0 32.0 36.0 26 34.602430899097435 36.0 36.0 36.0 32.0 36.0 27 34.608304446715536 36.0 36.0 36.0 32.0 36.0 28 34.57278030883267 36.0 36.0 36.0 32.0 36.0 29 34.54384092300601 36.0 36.0 36.0 32.0 36.0 30 34.51735757869227 36.0 36.0 36.0 32.0 36.0 31 34.49966942379636 36.0 36.0 36.0 32.0 36.0 32 34.459804727239714 36.0 36.0 36.0 32.0 36.0 33 34.42805427968143 36.0 36.0 36.0 32.0 36.0 34 34.402169231736245 36.0 36.0 36.0 32.0 36.0 35 34.34911175105284 36.0 36.0 36.0 32.0 36.0 36 34.31068226738028 36.0 36.0 36.0 32.0 36.0 37 34.29215952397027 36.0 36.0 36.0 32.0 36.0 38 34.24348799798861 36.0 36.0 36.0 32.0 36.0 39 34.215543832775 36.0 36.0 36.0 32.0 36.0 40 34.197476213645345 36.0 36.0 36.0 32.0 36.0 41 34.167314627065814 36.0 36.0 36.0 32.0 36.0 42 34.089335891030906 36.0 36.0 36.0 32.0 36.0 43 34.08065011768047 36.0 36.0 36.0 32.0 36.0 44 34.0011418847034 36.0 36.0 36.0 32.0 36.0 45 33.94637215896525 36.0 36.0 36.0 32.0 36.0 46 33.91040802881135 36.0 36.0 36.0 32.0 36.0 47 33.87611307568565 36.0 36.0 36.0 32.0 36.0 48 33.82412531166119 36.0 36.0 36.0 32.0 36.0 49 33.776238322162804 36.0 36.0 36.0 32.0 36.0 50 33.10510460874444 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 5.0 20 8.0 21 25.0 22 107.0 23 298.0 24 746.0 25 1804.0 26 3499.0 27 6595.0 28 11309.0 29 17566.0 30 25919.0 31 38084.0 32 56891.0 33 92690.0 34 182337.0 35 421223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.913500859671565 18.6860120901136 12.385556586411251 26.014930463803577 2 15.385233202537997 20.74605722480308 37.998819699151326 25.869889873507603 3 18.420375670903255 24.920411173049096 28.776875415404206 27.882337740643436 4 12.067893833822602 16.297639639346016 36.33125598005368 35.3032105467777 5 13.912951370378043 37.41878184996962 33.3220813264021 15.34618545325024 6 33.83742383061442 36.11816949034169 16.78351307465327 13.260893604390617 7 29.72756531506626 30.638396936346545 21.072511509070484 18.561526239516706 8 27.624065016424048 34.11208861304659 19.29668748675949 18.967158883769873 9 27.285340969275808 14.224038086591124 18.6624296498059 39.82819129432717 10 15.530591387711299 27.325753290078662 32.17921587525521 24.964439446954827 11 36.959001566744966 21.054095769322995 23.036854590702426 18.950048073229613 12 25.030671418893597 23.700567799549766 29.438160133906642 21.83060064765 13 29.848772674786755 20.046816217826947 25.42113643982568 24.68327466756062 14 23.002390868604657 20.267325649340822 25.75503258053177 30.975250901522756 15 24.3678091344654 28.661492114783794 22.649334866763827 24.321363883986983 16 24.626294089847725 26.039520520359726 25.277558555601342 24.05662683419121 17 23.06921381063571 26.62919360358326 26.15672571254304 24.144866873237994 18 23.88689132734957 25.15588934039341 27.97115130548956 22.98606802676745 19 25.18955751676743 25.05173983187174 26.13542449942149 23.623278151939342 20 25.70413559258902 24.600891860463765 25.976396311036044 23.718576235911176 21 25.80962069872635 24.62082676642929 25.058956636317287 24.510595898527075 22 25.231258111639438 25.358017937271924 25.67765290622217 23.73307104486646 23 23.666949520487112 25.23975644307247 26.485826462779116 24.607467573661303 24 24.501105808404144 25.35583750436503 26.31870562216273 23.824351065068093 25 24.343319822976298 25.28372913790575 26.35602157621867 24.016929462899288 26 23.39720905543708 26.300485179218313 26.744456757587326 23.55784900775728 27 24.16073866870611 25.77285145723402 25.988203371363316 24.078206502696556 28 23.4389859767447 25.743563119781438 26.354919779809165 24.462531123664693 29 23.702216324440098 25.57887507566234 26.24703170833915 24.471876891558413 30 23.671994133190545 25.690223148288265 26.504475770309753 24.133306948211438 31 24.44359854583044 25.44459150663468 25.706626063533193 24.40518388400169 32 23.818265205872354 26.00875824723307 25.663969194809304 24.509007352085263 33 23.215605658657644 25.84879282668644 26.426974191948766 24.50862732270715 34 24.068859630232772 25.77836082977042 26.563036547194034 23.589742992802773 35 24.509242445406716 25.917609535782464 26.22235414629604 23.35079387251478 36 23.545243316989584 26.199589310908404 26.209716848031068 24.045450524070944 37 24.30937425792363 26.12655923340892 25.442568527115178 24.121497981552274 38 23.939872975666084 26.316108063810173 25.73185665993053 24.012162300593214 39 24.410089503661514 25.607196611223053 25.624191714072523 24.358522171042907 40 24.905364318273882 25.811096470193835 26.01212454195399 23.271414669578295 41 24.058225755759135 25.791262644487645 26.443945196549755 23.706566403203464 42 24.675761561652447 26.683118183193844 25.799159575830238 22.84196067932347 43 23.780434572580276 25.896493643907498 26.15897855539014 24.164093228122084 44 23.49812709668558 26.40502669090926 25.52991872833473 24.566927484070426 45 23.639637710091247 26.721965426247046 25.506960263813728 24.13143659984798 46 23.598615755845962 25.867742525000086 25.744240755118458 24.789400964035494 47 24.37890168935947 25.711368982973703 25.8115921278852 24.09813719978163 48 24.25152837208003 26.429080276201162 25.134445525699466 24.18494582601934 49 24.096994190452133 26.24400101035615 25.37251063027367 24.286494168918047 50 23.906542643527374 26.99051434837217 24.90935143937692 24.19359156872354 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 44.0 1 36.5 2 29.0 3 109.0 4 189.0 5 206.0 6 223.0 7 223.5 8 224.0 9 300.0 10 376.0 11 571.5 12 767.0 13 1278.0 14 1789.0 15 2406.5 16 3024.0 17 3342.0 18 3660.0 19 3854.0 20 4048.0 21 4545.0 22 5042.0 23 5704.5 24 6367.0 25 6869.0 26 7371.0 27 8846.0 28 10321.0 29 12275.5 30 14230.0 31 16766.0 32 19302.0 33 22870.5 34 26439.0 35 30526.0 36 34613.0 37 37050.5 38 39488.0 39 40496.5 40 41505.0 41 43125.0 42 44745.0 43 45428.5 44 46112.0 45 49479.0 46 52846.0 47 56093.0 48 59340.0 49 60382.0 50 61424.0 51 59190.5 52 56957.0 53 56159.5 54 55362.0 55 55160.0 56 54958.0 57 53189.5 58 51421.0 59 47175.0 60 42929.0 61 37962.0 62 32995.0 63 28665.5 64 24336.0 65 20970.5 66 17605.0 67 15101.5 68 12598.0 69 11343.5 70 10089.0 71 7863.0 72 5637.0 73 4863.5 74 4090.0 75 3183.0 76 2276.0 77 2004.0 78 1732.0 79 1369.5 80 1007.0 81 848.5 82 690.0 83 571.0 84 452.0 85 329.0 86 206.0 87 150.5 88 95.0 89 73.5 90 52.0 91 46.5 92 41.0 93 30.5 94 20.0 95 19.0 96 18.0 97 14.0 98 10.0 99 11.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006634804087039318 2 3.4920021510733253E-4 3 3.4920021510733253E-4 4 0.0 5 0.0 6 1.1640007170244418E-4 7 1.1640007170244418E-4 8 0.0 9 0.001280400788726886 10 9.312005736195534E-4 11 0.0 12 0.0 13 2.3280014340488835E-4 14 4.656002868097767E-4 15 0.003492002151073325 16 9.312005736195534E-4 17 0.0 18 3.4920021510733253E-4 19 0.0 20 3.4920021510733253E-4 21 0.0 22 1.1640007170244418E-4 23 5.820003585122208E-4 24 6.984004302146651E-4 25 0.0013968008604293301 26 0.004888803011502655 27 0.005470803370014875 28 0.0038412023661806576 29 0.0030264018642635485 30 0.00419040258128799 31 0.006867604230444206 32 0.003492002151073325 33 0.0038412023661806576 34 0.002910001792561104 35 0.0030264018642635485 36 0.007216804445551539 37 0.003492002151073325 38 0.006751204158741762 39 0.004074002509585546 40 0.002560801577453772 41 0.00419040258128799 42 0.0018624011472391068 43 0.0010476006453219975 44 0.0013968008604293301 45 0.0017460010755366625 46 0.0010476006453219975 47 0.002677201649156216 48 0.002560801577453772 49 3.4920021510733253E-4 50 0.0022116013623464394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 859106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.00761383098948 #Duplication Level Percentage of deduplicated Percentage of total 1 80.85447298572134 51.753018833782235 2 12.413321971912229 15.890942382757897 3 3.402914874317014 6.534373835250406 4 1.2723988991169062 3.257728694946043 5 0.6023663230096175 1.9278015493996332 6 0.33025876024259193 1.2683445113945502 7 0.21524697620866917 0.9644211732016874 8 0.1430578704475491 0.7325434341672357 9 0.10338650849997981 0.5955771340260919 >10 0.5429713459689502 6.570305443454082 >50 0.06455864780461895 2.964403009226871 >100 0.052118865391507944 6.357838729285977 >500 0.002925971359054871 1.1827012691072578 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 933 0.1086012668983804 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 4.6560028680977665E-4 13 0.0 0.0 0.0 0.0 5.820003585122208E-4 14 1.1640007170244416E-4 0.0 0.0 0.0 5.820003585122208E-4 15 1.1640007170244416E-4 0.0 0.0 0.0 6.984004302146651E-4 16 1.1640007170244416E-4 0.0 0.0 0.0 6.984004302146651E-4 17 1.1640007170244416E-4 0.0 0.0 0.0 6.984004302146651E-4 18 1.1640007170244416E-4 0.0 0.0 1.1640007170244416E-4 0.0011640007170244417 19 1.1640007170244416E-4 0.0 0.0 2.3280014340488833E-4 0.0011640007170244417 20 1.1640007170244416E-4 0.0 0.0 2.3280014340488833E-4 0.0013968008604293301 21 1.1640007170244416E-4 0.0 0.0 4.6560028680977665E-4 0.0013968008604293301 22 1.1640007170244416E-4 0.0 0.0 8.148005019171092E-4 0.0013968008604293301 23 1.1640007170244416E-4 0.0 0.0 0.0017460010755366625 0.0013968008604293301 24 1.1640007170244416E-4 0.0 0.0 0.002677201649156216 0.0013968008604293301 25 1.1640007170244416E-4 0.0 0.0 0.003375602079370881 0.0013968008604293301 26 1.1640007170244416E-4 0.0 0.0 0.005121603154907543 0.0013968008604293301 27 1.1640007170244416E-4 0.0 0.0 0.0074496045889564265 0.0015132009321317742 28 1.1640007170244416E-4 0.0 0.0 0.010359606381517531 0.0016296010038342184 29 1.1640007170244416E-4 0.0 0.0 0.015481209536425074 0.0016296010038342184 30 1.1640007170244416E-4 0.0 0.0 0.025840815917942607 0.0016296010038342184 31 1.1640007170244416E-4 0.0 0.0 0.04993563076034855 0.0016296010038342184 32 1.1640007170244416E-4 0.0 0.0 0.07810444811234003 0.0016296010038342184 33 1.1640007170244416E-4 0.0 0.0 0.10604046532092663 0.0016296010038342184 34 1.1640007170244416E-4 0.0 0.0 0.1307172805218448 0.0016296010038342184 35 1.1640007170244416E-4 0.0 0.0 0.1655209019608756 0.0016296010038342184 36 1.1640007170244416E-4 0.0 0.0 0.20905452877758973 0.0016296010038342184 37 1.1640007170244416E-4 0.0 0.0 0.27295816814223156 0.0016296010038342184 38 1.1640007170244416E-4 0.0 0.0 0.3550202186924547 0.0016296010038342184 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 30 0.005745772 29.331585 12 TGTAGGA 770 0.0 21.141598 2 CTGTAGG 730 0.0 20.49551 1 GTAGGAC 755 0.0 19.230644 3 GGCGTTA 210 0.0 18.857119 42 GATATAC 180 1.4551915E-11 18.335443 1 CGCGGGA 170 1.2551027E-10 18.117622 44 GTCCTAT 220 0.0 18.002071 1 TTCGTAT 260 0.0 17.769207 25 TAGGACG 695 0.0 17.725563 4 TAGGACC 575 0.0 17.598953 4 TCTATAG 100 2.482927E-5 17.598953 3 GACGTGA 415 0.0 17.492935 7 CGCAATA 215 0.0 17.396338 36 CGGCGTT 230 0.0 17.21737 41 ATTTCGT 320 0.0 17.187477 42 GGACGTG 735 0.0 17.06021 6 GTCCTAC 645 0.0 17.056227 1 TAGCGGC 220 1.8189894E-12 17.001957 30 AGCGAAA 220 1.8189894E-12 16.999977 17 >>END_MODULE