FastQCFastQC Report
Thu 2 Feb 2017
SRR4063928_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063928_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712280
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56850.2096022534546581No Hit
TATCAACGCAGAGTACTTTTTTTTT36720.13538425236332532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1800.013.7219427
AATCGGT701.08962005E-412.21651619
GTATCAA37500.012.1355391
ACTCTAA4100.012.04942410
AGAACCG2400.011.87278655
CGCGTAA1459.822543E-1111.79373410
TAGGACA4200.011.7592784
AGTATCG658.0244493E-411.6927168
GCGTAAC1552.5465852E-1111.64578311
CGAGCCG4950.011.516191515
CAAGACG6600.011.3686744
AAGACGG6750.011.257165
GTTCGAC600.005875234511.08392511
GTAGGAC9000.010.9745183
CGAACGA2000.010.92578516
GGTATCA14350.010.85792351
TAGGACG5950.010.8547194
CGTCGTA4700.010.71333910
GCGTTAT1605.4387783E-1010.6882681
CGGACCA6700.010.6345039
Produced by FastQC (version 0.11.5)