##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063928_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2712280
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317855457401155	32.0	32.0	32.0	32.0	32.0
2	31.009616632501068	32.0	32.0	32.0	32.0	32.0
3	31.024086746206144	32.0	32.0	32.0	32.0	32.0
4	31.052817924403087	32.0	32.0	32.0	32.0	32.0
5	30.979154069638827	32.0	32.0	32.0	32.0	32.0
6	34.61546411137493	36.0	36.0	36.0	32.0	36.0
7	34.567389428820036	36.0	36.0	36.0	32.0	36.0
8	34.54515536743994	36.0	36.0	36.0	32.0	36.0
9	34.65190651407672	36.0	36.0	36.0	32.0	36.0
10	34.428391980179036	36.0	36.0	36.0	32.0	36.0
11	34.60601117878685	36.0	36.0	36.0	32.0	36.0
12	34.47953456132848	36.0	36.0	36.0	32.0	36.0
13	34.5415215243264	36.0	36.0	36.0	32.0	36.0
14	34.46573178285428	36.0	36.0	36.0	32.0	36.0
15	34.40759324258558	36.0	36.0	36.0	32.0	36.0
16	34.403656333416905	36.0	36.0	36.0	32.0	36.0
17	34.341537009453305	36.0	36.0	36.0	32.0	36.0
18	34.33666140663943	36.0	36.0	36.0	32.0	36.0
19	34.34058098721371	36.0	36.0	36.0	32.0	36.0
20	34.31355464775023	36.0	36.0	36.0	32.0	36.0
21	34.30089739997345	36.0	36.0	36.0	32.0	36.0
22	34.27994270503046	36.0	36.0	36.0	32.0	36.0
23	34.233233294497616	36.0	36.0	36.0	32.0	36.0
24	34.21264766174584	36.0	36.0	36.0	32.0	36.0
25	33.81180704057103	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	24.0
5	33.0
6	116.0
7	38.0
8	150.0
9	169.0
10	238.0
11	81.0
12	177.0
13	161.0
14	211.0
15	442.0
16	783.0
17	1080.0
18	1349.0
19	1758.0
20	2455.0
21	3273.0
22	4770.0
23	7222.0
24	10744.0
25	15949.0
26	23164.0
27	31797.0
28	44623.0
29	62665.0
30	86213.0
31	121266.0
32	178688.0
33	262729.0
34	585243.0
35	1264658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43206361278202	16.99511558935838	11.413543202304153	26.15927759555546
2	16.724579242766026	19.508070028988243	37.86136673187977	25.90598399636596
3	19.05511770379525	23.633745195838703	28.199947497116767	29.111189603249283
4	12.690571501575675	15.470005394202651	35.739671281788645	36.09975182243302
5	14.757802570258438	36.37365907295034	33.447135219723435	15.421403137067792
6	34.21403133299064	35.240432729501286	17.02093217232776	13.524603765180304
7	30.602959962473115	30.085527072755543	20.623965576754575	18.68754738801677
8	28.02201429631004	33.03077568233143	19.17554085099964	19.77166917035889
9	27.648775830975296	14.213477903060229	18.230496347947078	39.9072499180174
10	15.916351622147259	26.381068111592164	31.443693354700287	26.258886911560293
11	37.199088240643086	20.89358761960939	22.71769656444972	19.1896275752978
12	25.379189792644645	23.061734370872557	28.73058118823132	22.82849464825148
13	29.701225365591625	19.40777070825054	25.014775664462185	25.87622826169565
14	23.83370694654945	19.554777994058593	24.938682175919435	31.672832883472523
15	25.255650617401916	27.06113295307775	22.047802118545743	25.635414310974596
16	25.765989992672704	25.489168813325197	23.80983126562953	24.935009928372565
17	24.149935307831054	25.62990471601669	25.116573185529468	25.10358679062279
18	24.87527604204141	24.746170464377894	26.098144591605372	24.280408901975324
19	25.785903384405355	24.468927856543278	25.188019444880037	24.557149314171333
20	26.094495127131466	24.12313457618663	24.673260253834904	25.109110042847
21	26.986326742807133	23.844148924787124	24.183305328578058	24.986219003827685
22	26.198224764738526	24.159574876317997	24.60947185717828	25.0327285017652
23	24.678951419654275	24.127074795592367	25.189389326892066	26.00458445786129
24	25.041603108923766	24.662249172919125	25.064258815246777	25.231888902910338
25	25.045228140593007	24.310988067445738	25.12511304729002	25.518670744671233
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	153.0
1	153.0
2	440.0
3	727.0
4	727.0
5	727.0
6	1922.5
7	3118.0
8	3118.0
9	3118.0
10	3435.5
11	3753.0
12	3753.0
13	3753.0
14	4718.0
15	5683.0
16	5683.0
17	5683.0
18	10248.5
19	14814.0
20	14814.0
21	14814.0
22	24887.0
23	34960.0
24	34960.0
25	34960.0
26	53180.5
27	71401.0
28	71401.0
29	71401.0
30	91924.5
31	112448.0
32	112448.0
33	112448.0
34	139613.0
35	166778.0
36	166778.0
37	166778.0
38	192277.5
39	217777.0
40	217777.0
41	217777.0
42	251634.0
43	285491.0
44	285491.0
45	285491.0
46	317810.5
47	350130.0
48	350130.0
49	350130.0
50	361816.5
51	373503.0
52	373503.0
53	373503.0
54	348958.5
55	324414.0
56	324414.0
57	324414.0
58	298549.0
59	272684.0
60	272684.0
61	272684.0
62	239138.0
63	205592.0
64	205592.0
65	205592.0
66	167956.5
67	130321.0
68	130321.0
69	130321.0
70	98637.0
71	66953.0
72	66953.0
73	66953.0
74	51090.5
75	35228.0
76	35228.0
77	35228.0
78	27889.5
79	20551.0
80	20551.0
81	20551.0
82	14225.0
83	7899.0
84	7899.0
85	7899.0
86	6031.0
87	4163.0
88	4163.0
89	4163.0
90	2973.0
91	1783.0
92	1783.0
93	1783.0
94	1201.0
95	619.0
96	619.0
97	619.0
98	978.0
99	1337.0
100	1337.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03690621912191957
2	0.005382925066733523
3	0.001769728789063076
4	0.0040187591251640686
5	0.009475422891441886
6	0.013936614213871726
7	0.02300647425781999
8	0.03506275163331219
9	0.049368059344905395
10	0.057221230846372796
11	0.05445602961346174
12	0.06503753299806805
13	0.0642264073030808
14	0.06680726178713112
15	0.0607238190747268
16	0.06879820667482708
17	0.06455823145103012
18	0.07724128777264884
19	0.07576651378176294
20	0.08008022770510419
21	0.07469730263837067
22	0.07757311192059817
23	0.08310351438642029
24	0.07912162461102835
25	0.07856858436444615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2712280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.78773684895023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77968426226809	35.257641363239024
2	14.661584554783294	14.012878889852342
3	4.918172033902639	7.0508493280201705
4	2.1546197587686944	4.1185760816634795
5	1.1219984350629395	2.680888297986086
6	0.7172587980910942	2.0565704817462915
7	0.4739375538726088	1.585388217510958
8	0.34158457324773284	1.3058842958418897
9	0.2443826368728173	1.051064382719768
>10	1.4011898612185416	12.06702983178087
>50	0.0949511204074626	3.156259894219719
>100	0.07314604272574705	7.428270426332947
>500	0.012072997918052183	4.012145591889622
>1k	0.005339979848362695	4.006302212584481
>5k	7.739101229511153E-5	0.21025070461235676
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5685	0.2096022534546581	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3672	0.13538425236332532	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.686934977214742E-5
2	0.0	0.0	0.0	0.0	3.686934977214742E-5
3	0.0	0.0	0.0	0.0	3.686934977214742E-5
4	0.0	0.0	0.0	0.0	3.686934977214742E-5
5	0.0	0.0	0.0	0.0	3.686934977214742E-5
6	0.0	0.0	0.0	3.686934977214742E-5	7.373869954429484E-5
7	0.0	0.0	0.0	3.686934977214742E-5	7.373869954429484E-5
8	0.0	0.0	0.0	3.686934977214742E-5	7.373869954429484E-5
9	0.0	0.0	0.0	7.373869954429484E-5	7.373869954429484E-5
10	0.0	0.0	0.0	7.373869954429484E-5	7.373869954429484E-5
11	0.0	0.0	0.0	1.1060804931644226E-4	7.373869954429484E-5
12	0.0	0.0	0.0	1.1060804931644226E-4	2.5808544840503193E-4
13	0.0	0.0	0.0	1.4747739908858967E-4	3.3182414794932677E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	180	0.0	13.721942	7
AATCGGT	70	1.08962005E-4	12.216516	19
GTATCAA	3750	0.0	12.135539	1
ACTCTAA	410	0.0	12.049424	10
AGAACCG	240	0.0	11.8727865	5
CGCGTAA	145	9.822543E-11	11.793734	10
TAGGACA	420	0.0	11.759278	4
AGTATCG	65	8.0244493E-4	11.692716	8
GCGTAAC	155	2.5465852E-11	11.645783	11
CGAGCCG	495	0.0	11.5161915	15
CAAGACG	660	0.0	11.368674	4
AAGACGG	675	0.0	11.25716	5
GTTCGAC	60	0.0058752345	11.083925	11
GTAGGAC	900	0.0	10.974518	3
CGAACGA	200	0.0	10.925785	16
GGTATCA	1435	0.0	10.8579235	1
TAGGACG	595	0.0	10.854719	4
CGTCGTA	470	0.0	10.713339	10
GCGTTAT	160	5.4387783E-10	10.688268	1
CGGACCA	670	0.0	10.634503	9
>>END_MODULE