FastQCFastQC Report
Thu 2 Feb 2017
SRR4063928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712280
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41550.023.8256651
GGTATCA20100.019.8100741
TAGGACG8350.018.178844
TAATACT5100.018.116854
TACGAAT7450.018.01296241
ATACGAA7100.017.9713740
CGCAATA7250.017.90464236
CTAGCGG7450.017.71832329
CGAGCCG9700.017.68994315
AACGCAG55850.017.4495476
CGTCTTA2850.016.9817115
GATATAC4350.016.6889211
GTCCTAC11300.016.5479451
TCAACGC59800.016.3705184
TAGCGGC7800.016.36006430
TCTAGCG7800.016.36006428
TATCAAC60700.016.344642
CAATACG7950.016.3275338
GTTACGT953.346536E-416.21160531
ATCAACG60400.016.1711773
Produced by FastQC (version 0.11.5)