##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063928_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2712280
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310242305366703	32.0	32.0	32.0	32.0	32.0
2	31.310436238146504	32.0	32.0	32.0	32.0	32.0
3	31.41954223015323	32.0	32.0	32.0	32.0	32.0
4	31.518646304953766	32.0	32.0	32.0	32.0	32.0
5	31.43531715014674	32.0	32.0	32.0	32.0	32.0
6	35.03954311501762	36.0	36.0	36.0	36.0	36.0
7	35.05693770554662	36.0	36.0	36.0	36.0	36.0
8	34.99721894494669	36.0	36.0	36.0	36.0	36.0
9	35.13749502263778	36.0	36.0	36.0	36.0	36.0
10	34.95946362469952	36.0	36.0	36.0	36.0	36.0
11	35.13558039730411	36.0	36.0	36.0	36.0	36.0
12	35.02825961921336	36.0	36.0	36.0	36.0	36.0
13	35.078470143200555	36.0	36.0	36.0	36.0	36.0
14	35.02340982494433	36.0	36.0	36.0	36.0	36.0
15	34.980640273128145	36.0	36.0	36.0	36.0	36.0
16	34.99906757414426	36.0	36.0	36.0	36.0	36.0
17	34.95233641069506	36.0	36.0	36.0	36.0	36.0
18	34.95532983320306	36.0	36.0	36.0	36.0	36.0
19	34.95528264043536	36.0	36.0	36.0	36.0	36.0
20	34.9471518427301	36.0	36.0	36.0	36.0	36.0
21	34.94701579482944	36.0	36.0	36.0	36.0	36.0
22	34.92124891235418	36.0	36.0	36.0	32.0	36.0
23	34.87492589260696	36.0	36.0	36.0	32.0	36.0
24	34.844718834338636	36.0	36.0	36.0	32.0	36.0
25	34.813992655625526	36.0	36.0	36.0	32.0	36.0
26	34.75193121654107	36.0	36.0	36.0	32.0	36.0
27	34.74813736044951	36.0	36.0	36.0	32.0	36.0
28	34.721575574793164	36.0	36.0	36.0	32.0	36.0
29	34.69792351822083	36.0	36.0	36.0	32.0	36.0
30	34.67750011060805	36.0	36.0	36.0	32.0	36.0
31	34.66959384724291	36.0	36.0	36.0	32.0	36.0
32	34.62696771719734	36.0	36.0	36.0	32.0	36.0
33	34.60182540150722	36.0	36.0	36.0	32.0	36.0
34	34.60282456088604	36.0	36.0	36.0	32.0	36.0
35	34.56472045659003	36.0	36.0	36.0	32.0	36.0
36	34.536538631704694	36.0	36.0	36.0	32.0	36.0
37	34.52426740602003	36.0	36.0	36.0	32.0	36.0
38	34.48771218310794	36.0	36.0	36.0	32.0	36.0
39	34.4785597357205	36.0	36.0	36.0	32.0	36.0
40	34.46298722845724	36.0	36.0	36.0	32.0	36.0
41	34.44706667453213	36.0	36.0	36.0	32.0	36.0
42	34.38568989927294	36.0	36.0	36.0	32.0	36.0
43	34.38003819664636	36.0	36.0	36.0	32.0	36.0
44	34.31701446753285	36.0	36.0	36.0	32.0	36.0
45	34.28455948500892	36.0	36.0	36.0	32.0	36.0
46	34.25894302948073	36.0	36.0	36.0	32.0	36.0
47	34.23874526228855	36.0	36.0	36.0	32.0	36.0
48	34.188778813396844	36.0	36.0	36.0	32.0	36.0
49	34.16881442918873	36.0	36.0	36.0	32.0	36.0
50	33.53268320379902	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	2.0
20	23.0
21	84.0
22	232.0
23	753.0
24	1955.0
25	4458.0
26	9507.0
27	17749.0
28	30002.0
29	47390.0
30	69794.0
31	102232.0
32	150989.0
33	250107.0
34	519778.0
35	1507220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.409529020829986	17.974802843013336	12.014482023206046	26.601186112950636
2	15.918080366689427	20.315027865977846	37.343544222031156	26.423347545301574
3	18.33910340784656	24.31074339944032	28.526216047812348	28.823937144900764
4	12.368376421313434	16.135797189080773	35.91000929107614	35.585817098529645
5	14.454149276623356	36.85633489167785	33.13260430339051	15.556911528308287
6	34.29250246471785	35.60969782596032	16.729406056458814	13.368393652863016
7	30.522767401707124	30.29937576434855	20.534480807085707	18.64337602685861
8	28.059576466875274	33.2470958924211	19.035467958864114	19.657859681839515
9	27.773515228852176	14.071106198426179	18.039209469028147	40.1161691036935
10	15.844714059598248	26.611930046555255	31.40254569895744	26.140810194889063
11	37.40421793201801	20.84650645933288	22.635556766510206	19.113718842138912
12	25.41779533254949	23.141549541657493	28.57201321548384	22.868641910309176
13	29.77170414449889	19.42384054684821	24.980809432689224	25.823645875963674
14	23.913597320627648	19.462429537985983	24.932814753065415	31.691158388320957
15	25.38216940963399	27.167248984937398	21.871040960891513	25.579540644537097
16	25.865155208259978	25.505455046881586	23.6694209513177	24.959968793540735
17	24.291223619980236	25.688756323093486	24.987538159777014	25.03248189714926
18	25.040408926538344	24.70629789342662	25.9879171410537	24.26537603898134
19	25.93593800637766	24.524246952470598	25.07769296595225	24.462122075199492
20	26.242155072039502	24.086058057813	24.660043904313078	25.011742965834422
21	27.174369902812394	23.842707980002064	24.102894981344107	24.88002713584143
22	26.28983923465016	24.223087915831577	24.530984309856372	24.95608853966189
23	24.739027311939836	24.03393188772303	25.167710876955983	26.059329923381153
24	25.18667990530417	24.60823305114585	25.007567481472115	25.197519562077865
25	25.327543761911308	24.223988130813247	25.10352171070848	25.344946396566964
26	24.86102349096602	25.024731310853497	25.448749312814307	24.66549588536618
27	25.466647395092828	24.602155073241814	24.704879444506716	25.226318087158646
28	24.79559702628931	24.591258576628945	25.253017236770447	25.36012716031129
29	24.886697981727146	24.51519419516116	25.119274985067584	25.478832838044113
30	24.74184117835533	24.733471548975054	25.368531004683675	25.156156267985946
31	25.475792261960066	24.448915751142653	24.50577189403962	25.569520092857662
32	25.179595900007374	24.74869109947644	24.366012830912176	25.70570016960401
33	24.728178811866076	24.404673897533673	25.13040300954697	25.73674428105328
34	25.48668690104328	24.436733652481955	25.232024293050404	24.84455515342436
35	25.87112002884745	24.38900109800243	25.117487735039994	24.62239113811012
36	24.87693919856169	24.846593418911137	24.874874358148045	25.401593024379128
37	25.743364965780476	24.60012078781975	24.549940011665832	25.106574234733948
38	25.08168100070979	24.571370629499555	24.951079892702083	25.395868477088573
39	25.70993707269105	24.341331705121018	24.50219878717355	25.44653243501438
40	25.926121200814382	24.490510746523338	24.92602017678468	24.6573478758776
41	24.933107833822053	24.791672888550664	25.374446805380575	24.90077247224671
42	25.988436851204376	25.01857322940436	24.815567371140503	24.177422548250764
43	25.219577841275694	24.2541432390082	25.12290533689741	25.403373582818695
44	24.869250573970163	24.795584771162236	24.662595506533506	25.672569148334095
45	25.084321197766574	25.00390819718551	24.736786606534505	25.17498399851341
46	25.062355862557173	24.595032546718507	24.751839336640543	25.590772254083777
47	25.33625442071327	24.546757062080236	25.105890646522866	25.011097870683628
48	25.74201719246304	25.031496164602256	24.24329012788346	24.98319651505124
49	25.150639334609753	24.991178985301257	24.46206558115647	25.39611609893252
50	25.1339759261806	25.31050891126563	24.363174216450528	25.192340946103243
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	30.0
2	30.0
3	107.5
4	185.0
5	227.0
6	269.0
7	287.5
8	306.0
9	399.5
10	493.0
11	746.0
12	999.0
13	1862.0
14	2725.0
15	3734.5
16	4744.0
17	5116.0
18	5488.0
19	5506.0
20	5524.0
21	6291.0
22	7058.0
23	8286.0
24	9514.0
25	11378.5
26	13243.0
27	17336.0
28	21429.0
29	25782.5
30	30136.0
31	35239.0
32	40342.0
33	47795.5
34	55249.0
35	65157.0
36	75065.0
37	86800.0
38	98535.0
39	106935.0
40	115335.0
41	125646.0
42	135957.0
43	140814.0
44	145671.0
45	159401.0
46	173131.0
47	185601.5
48	198072.0
49	205882.0
50	213692.0
51	210022.5
52	206353.0
53	204069.5
54	201786.0
55	201841.5
56	201897.0
57	196892.5
58	191888.0
59	174632.0
60	157376.0
61	138447.0
62	119518.0
63	103713.5
64	87909.0
65	74615.5
66	61322.0
67	52307.0
68	43292.0
69	38756.0
70	34220.0
71	26602.0
72	18984.0
73	16044.5
74	13105.0
75	10199.0
76	7293.0
77	6407.0
78	5521.0
79	4577.5
80	3634.0
81	2949.0
82	2264.0
83	1812.5
84	1361.0
85	1008.0
86	655.0
87	448.5
88	242.0
89	197.0
90	152.0
91	134.0
92	116.0
93	84.5
94	53.0
95	58.0
96	63.0
97	45.5
98	28.0
99	27.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00840621174804961
2	4.42432197265769E-4
3	3.686934977214742E-4
4	0.0
5	0.0
6	7.373869954429484E-5
7	1.1060804931644226E-4
8	3.686934977214742E-5
9	9.954724438479801E-4
10	7.00517645670801E-4
11	1.1060804931644226E-4
12	2.5808544840503193E-4
13	3.686934977214742E-4
14	1.1060804931644226E-4
15	0.00486675416992346
16	7.742563452150957E-4
17	0.0
18	2.9495479817717935E-4
19	3.686934977214742E-5
20	6.636482958986535E-4
21	0.0
22	1.4747739908858967E-4
23	8.479950447593907E-4
24	6.267789461265062E-4
25	0.0017328594392909285
26	0.004829884820151312
27	0.006194050761720766
28	0.004166236524252658
29	0.0022121609863288453
30	0.00342884952880971
31	0.006341528160809356
32	0.0029495479817717935
33	0.004092497824708364
34	0.002359638385417435
35	0.003023286681316088
36	0.007595086053062368
37	0.003170764080404678
38	0.007558216703290221
39	0.003797543026531184
40	0.0019909448876959603
41	0.002912678631999646
42	0.001585382040202339
43	0.0011060804931644227
44	0.00114294984293657
45	0.0011798191927087174
46	9.217337443036854E-4
47	0.001769728789063076
48	0.0016591207397466338
49	2.5808544840503193E-4
50	0.0014379046411137494
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2712280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.58990144309976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38178471971491	38.88938844295056
2	13.945531217678974	14.388971621834584
3	4.573380268856472	7.078207119963675
4	2.046886318504252	4.223946537474546
5	1.0716526369872306	2.764322696170459
6	0.6375977024080549	1.9736161576547042
7	0.4480647631120393	1.6180931878353164
8	0.3016960545484184	1.2451575775939965
9	0.23603703513357582	1.0959414641516385
>10	1.2031573948690886	11.05224272278909
>50	0.08174235871653046	2.9282307981746967
>100	0.059448577939676006	6.434709569996492
>500	0.008992369841803965	3.165981873171901
>1k	0.004028581689112302	3.141190230238369
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1060804931644226E-4
2	0.0	0.0	0.0	0.0	1.1060804931644226E-4
3	0.0	0.0	0.0	0.0	1.1060804931644226E-4
4	0.0	0.0	0.0	0.0	1.1060804931644226E-4
5	0.0	0.0	0.0	0.0	1.1060804931644226E-4
6	0.0	0.0	0.0	0.0	1.1060804931644226E-4
7	0.0	0.0	0.0	0.0	1.1060804931644226E-4
8	0.0	0.0	0.0	0.0	1.1060804931644226E-4
9	0.0	0.0	0.0	0.0	1.1060804931644226E-4
10	0.0	0.0	0.0	0.0	1.1060804931644226E-4
11	0.0	0.0	0.0	3.686934977214742E-5	1.1060804931644226E-4
12	0.0	0.0	0.0	3.686934977214742E-5	4.055628474936216E-4
13	0.0	0.0	0.0	7.373869954429484E-5	4.42432197265769E-4
14	0.0	0.0	0.0	7.373869954429484E-5	4.42432197265769E-4
15	0.0	0.0	0.0	1.1060804931644226E-4	6.267789461265061E-4
16	0.0	0.0	0.0	1.1060804931644226E-4	6.636482958986535E-4
17	0.0	0.0	0.0	1.4747739908858967E-4	6.636482958986535E-4
18	0.0	0.0	0.0	1.843467488607371E-4	8.111256949872432E-4
19	0.0	0.0	0.0	1.843467488607371E-4	8.111256949872432E-4
20	0.0	0.0	0.0	1.843467488607371E-4	8.479950447593906E-4
21	0.0	0.0	0.0	2.9495479817717935E-4	9.217337443036854E-4
22	0.0	0.0	0.0	2.9495479817717935E-4	9.586030940758329E-4
23	0.0	0.0	0.0	5.161708968100639E-4	9.586030940758329E-4
24	0.0	0.0	0.0	6.267789461265061E-4	9.954724438479804E-4
25	0.0	0.0	0.0	7.742563452150957E-4	9.954724438479804E-4
26	0.0	0.0	0.0	8.479950447593906E-4	0.0010323417936201277
27	3.686934977214742E-5	0.0	0.0	0.0012535578922530121	0.001069211143392275
28	3.686934977214742E-5	0.0	0.0	0.002322769035645287	0.001069211143392275
29	3.686934977214742E-5	0.0	0.0	0.004534930021974132	0.001069211143392275
30	3.686934977214742E-5	0.0	0.0	0.008885513295087528	0.001069211143392275
31	3.686934977214742E-5	0.0	0.0	0.023375167755541465	0.0011798191927087174
32	3.686934977214742E-5	0.0	0.0	0.039929505803235656	0.0012904272420251597
33	3.686934977214742E-5	0.0	0.0	0.05523028595867683	0.0012904272420251597
34	3.686934977214742E-5	0.0	0.0	0.07351748344566195	0.0013641659415694544
35	3.686934977214742E-5	0.0	0.0	0.09445927411624169	0.0013641659415694544
36	3.686934977214742E-5	0.0	0.0	0.12568761337325055	0.0013641659415694544
37	3.686934977214742E-5	0.0	0.0	0.1728435117318271	0.0013641659415694544
38	3.686934977214742E-5	0.0	0.0	0.2370699190349079	0.0014379046411137494
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4155	0.0	23.825665	1
GGTATCA	2010	0.0	19.810074	1
TAGGACG	835	0.0	18.17884	4
TAATACT	510	0.0	18.11685	4
TACGAAT	745	0.0	18.012962	41
ATACGAA	710	0.0	17.97137	40
CGCAATA	725	0.0	17.904642	36
CTAGCGG	745	0.0	17.718323	29
CGAGCCG	970	0.0	17.689943	15
AACGCAG	5585	0.0	17.449547	6
CGTCTTA	285	0.0	16.98171	15
GATATAC	435	0.0	16.688921	1
GTCCTAC	1130	0.0	16.547945	1
TCAACGC	5980	0.0	16.370518	4
TAGCGGC	780	0.0	16.360064	30
TCTAGCG	780	0.0	16.360064	28
TATCAAC	6070	0.0	16.34464	2
CAATACG	795	0.0	16.32753	38
GTTACGT	95	3.346536E-4	16.211605	31
ATCAACG	6040	0.0	16.171177	3
>>END_MODULE