FastQCFastQC Report
Thu 2 Feb 2017
SRR4063927_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063927_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4754
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG1392.923853596970972No Hit
GCGCAAGACGGACCAGAGCGAAAGC240.5048380311316786No Hit
ACCATACTCCCCCCGGAACCCAAAG190.39966344131257886No Hit
GAATAGGACCGCGGTTCTATTTTGT170.357593605384939No Hit
GCGTTATTCCCATGACCCGCCGGGC160.33655868742111905No Hit
GATTAAGAGGGACGGCCGGGGGCAT150.3155237694572991No Hit
GTATCAACGCAGAGTACATGGGGAA140.2944888514934792No Hit
CACCAGGAGTGGAGCCTGCGGCTTA140.2944888514934792No Hit
ATTATATACACTGCTGATTTATGTG140.2944888514934792No Hit
GTATTGCGCCGCTAGAGGTGAAATT140.2944888514934792No Hit
CCTCTGAGCTGTGGAGCTGCTTTTA130.27345393352965924No Hit
TCGTAGTTCCGACCATAAACGATGC130.27345393352965924No Hit
CCCAAAGACTTTGGTTTCCCGGAAG130.27345393352965924No Hit
ATCCAAAGGTGAAACCTAAAAGTCA130.27345393352965924No Hit
GTCTAAAGATGTCTCCCTCTATCAC130.27345393352965924No Hit
GTACATGGGGAATGCACACCCATCA130.27345393352965924No Hit
GTATCTGATCGTCTTCGAACCTCCG120.2524190155658393No Hit
TATCAACGCAGAGTACATGGGGAAT120.2524190155658393No Hit
GGATTGACAGATTGATAGCTCTTTC120.2524190155658393No Hit
GAGTATGGTTGCAAAGCTGAAACTT110.23138409760201936No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC110.23138409760201936No Hit
ACTCTCTACTGTGCATTTTGAGACA110.23138409760201936No Hit
GGTTAATTCCGATAACGAACGAGAC110.23138409760201936No Hit
GACCAGAGCGAAAGCATTTGCCAAG100.2103491796381994No Hit
ATCAGATACCGTCGTAGTTCCGACC100.2103491796381994No Hit
GTATCAACGCAGAGTACATGGGCAG100.2103491796381994No Hit
GGCCATAGGTCAGTCTAAAGATGTC100.2103491796381994No Hit
GGGTAGGCACACGCTGAGCCAGTCA100.2103491796381994No Hit
ATCTCGGGTGGCTGAACGCCACTTG90.18931426167437948No Hit
TCTAAGAAGTTGGGGGACGCCGACC90.18931426167437948No Hit
CTGTAAGGGTGATGTCGTCATCGTC90.18931426167437948No Hit
CTTTGGGACTTAGCAAGCTCTCAGT90.18931426167437948No Hit
GCCATGCACCACCACCCACGGAATC90.18931426167437948No Hit
TATCAACGCAGAGTACATGGGCAGC90.18931426167437948No Hit
ACACTGACTGGCTCAGCGTGTGCCT90.18931426167437948No Hit
GGGTTCAACGGGTTACCCGCGCCTG90.18931426167437948No Hit
GAATAATGGAATAGGACCGCGGTTC90.18931426167437948No Hit
GCACAGAACTTGCCGGGTTGCTTTT90.18931426167437948No Hit
CACTACACATTCTCTTTTCACACAA90.18931426167437948No Hit
GAATTAACCAGACAAATCGCTCCAC90.18931426167437948No Hit
CATTAGATGGGATTCCTGAAGTGAT90.18931426167437948No Hit
CCCATGTCCCCCTGGTATTCAGGAG80.16827934371055953No Hit
ATTCAGAGCAGAAGGGGGTCCTGCA80.16827934371055953No Hit
GGATAGCAGTTGGTTTAGTGAGAAA80.16827934371055953No Hit
CCTTAGTCCTGGAGGAGTAGAGACA80.16827934371055953No Hit
GTCCCAGAGTTCCCTGGGCTTGCAA80.16827934371055953No Hit
GCTCTGGGGAGGAGGCTCCTGGGGA80.16827934371055953No Hit
CTCTGGGACTCTGCCTGTCTCTACT80.16827934371055953No Hit
GAACTACGACGGTATCTGATCGTCT80.16827934371055953No Hit
GGTCTGAAGGACTCCTTTCACCGAA80.16827934371055953No Hit
GGACAAGAAGACCTAGTGAATGATT70.1472444257467396No Hit
GTGCATGGCCGTTCTTAGTTGGTGG70.1472444257467396No Hit
GTATCAACGCAGAGTACATGGGGGC70.1472444257467396No Hit
GTCTTTGGGTTCCGGGGGGAGTATG70.1472444257467396No Hit
CACATGGATAATGGATCATGGAAAT70.1472444257467396No Hit
GTTCGAAGACGATCAGATACCGTCG70.1472444257467396No Hit
CTGGCATGCTAACTAGTTACGCGAC70.1472444257467396No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT70.1472444257467396No Hit
AGTTTCAGCTTTGCAACCATACTCC70.1472444257467396No Hit
GTACATGGGCATACCCATGTCCCCC70.1472444257467396No Hit
GTAGGCAGTAACAGCCCTGCATTTG70.1472444257467396No Hit
GCTAACTAGTTACGCGACCCCCGAG70.1472444257467396No Hit
CTCCTGGGGAGTCTGCATTTGTCTC70.1472444257467396No Hit
TCACAGACCTGTTATTGCTCAATCT70.1472444257467396No Hit
TCTTCGAACCTCCGACTTTCGTTCT70.1472444257467396No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA70.1472444257467396No Hit
CTCCACTCCTGGTGGTGCCCTTCCG70.1472444257467396No Hit
CGAATGGGGTTCAACGGGTTACCCG70.1472444257467396No Hit
ACGCAGAGTACATGGGGAATAATTG70.1472444257467396No Hit
GTACTAGGGATCCAATCTCCTGTCT70.1472444257467396No Hit
TCTAAGGGCATCACAGACCTGTTAT70.1472444257467396No Hit
TTCTTAGTTGGTGGAGCGATTTGTC70.1472444257467396No Hit
AGACTCTGGCATGCTAACTAGTTAC60.12620950778291964No Hit
CCCATGACCCGCCGGGCAGCTTCCG60.12620950778291964No Hit
CATCTAAGGGCATCACAGACCTGTT60.12620950778291964No Hit
ATCCACCTCTGAGCTGTGGAGCTGC60.12620950778291964No Hit
CCTTTGGATTATCAGTTAAATCAAA60.12620950778291964No Hit
CTCTTAATCATGGCCTCAGTTCCGA60.12620950778291964No Hit
TGCCTACCCTACGCCGGCAGGCGCG60.12620950778291964No Hit
CTCCTGTACTGGATGAAAAGCAAAT60.12620950778291964No Hit
ACCCGAGATTGAGCAATAACAGGTC60.12620950778291964No Hit
ACCATAAACGATGCCGACTGGCGAT60.12620950778291964No Hit
GTGGTGGTGCATGGCCGTTCTTAGT60.12620950778291964No Hit
GCCTGAGAGGCTCTTCCCAAACTCC60.12620950778291964No Hit
ATATTGAGGAGTGCCTCGGCAGCCT60.12620950778291964No Hit
GTACATGGGGAATAATTGCAATCCC60.12620950778291964No Hit
CTCTTCTAGAGCTCAAGGGGGTTTT60.12620950778291964No Hit
AAATATAGCAGGATCACCAAGCTGC60.12620950778291964No Hit
CCCTTACAGTTGAGGCTTCTTGTCA60.12620950778291964No Hit
GTAGGGTCTAGAGGAAGAATGCTGG60.12620950778291964No Hit
CCTTAGATCCACATGAGAAAAGGCC60.12620950778291964No Hit
AGTCAGTGTAGCGCGCGTGCAGCCC60.12620950778291964No Hit
ACCTAAAAGTCAGACGGGATGGCTG60.12620950778291964No Hit
CTTCTTAGAGGGACAAGTGGCGTTC60.12620950778291964No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG60.12620950778291964No Hit
GTATTCAGGAGAGAGACGACAGCCA60.12620950778291964No Hit
CATGGATAATGGATCATGGAAATGG60.12620950778291964No Hit
GCGTAGGGTAGGCACACGCTGAGCC60.12620950778291964No Hit
GTCTTCTTGTCCCTTTAAACTTTTG60.12620950778291964No Hit
TTACAGTTTTCTAAGAGGCACCCGG60.12620950778291964No Hit
CACGAATGGGGTTCAACGGGTTACC50.1051745898190997No Hit
ACACGGGAAACCTCACCCGGCCCGG50.1051745898190997No Hit
AGAAGAGATAGGGCCCATTTCCATG50.1051745898190997No Hit
CCCAAGAGAGTGTGAAAGCAATTGG50.1051745898190997No Hit
CTCTCAGGCAGGTCTGTGAAGTTAC50.1051745898190997No Hit
GAATAACGCCGCCGCATCGCCAGTC50.1051745898190997No Hit
CTTTTACACTTTACAGTTTTCTAAG50.1051745898190997No Hit
CCATTATGACAAGAAGCCTCAACTG50.1051745898190997No Hit
GATAATGGATCATGGAAATGGGCCC50.1051745898190997No Hit
CCCGGAAGCTGCCCGGCGGGTCATG50.1051745898190997No Hit
GTTCTATTTTGTTGGTTTTCGGAAC50.1051745898190997No Hit
CCCCCCGGAACCCAAAGACTTTGGT50.1051745898190997No Hit
GCCCCGGACATCTAAGGGCATCACA50.1051745898190997No Hit
ATTTAAAGGGTGCCAGAAAAGTCTT50.1051745898190997No Hit
GTGTGGAATCCCCATTCCTCAAAGC50.1051745898190997No Hit
CTTCTAGAGCTCAAGGGGGTTTTGC50.1051745898190997No Hit
GTTCCCTGGGCTTGCAAGCCAAGTT50.1051745898190997No Hit
GGCACACGCTGAGCCAGTCAGTGTA50.1051745898190997No Hit
GGGCTGTTACTGCCTACTGTGCAAG50.1051745898190997No Hit
GTGCATCAGCGGCTCCTGAATCTGC50.1051745898190997No Hit
GCCAAGAATGTTTTCATTAATCAAG50.1051745898190997No Hit
CCTCTAGACCCTACCGTGCACATTA50.1051745898190997No Hit
GCTTAGGCCTTTTCTCATGTGGATC50.1051745898190997No Hit
TGATTAAGAGGGACGGCCGGGGGCA50.1051745898190997No Hit
GTGCACATGCACCTAGAACCTTAGT50.1051745898190997No Hit
GAAAATAATGTGCACGGTAGGGTCT50.1051745898190997No Hit
CCACCACCCACGGAATCGAGAAAGA50.1051745898190997No Hit
TCCCTTGAGTCCTCAACATGCAGGA50.1051745898190997No Hit
ACCCAAGAGAGTGTGAAAGCAATTG50.1051745898190997No Hit
ACTAAGAACGGCCATGCACCACCAC50.1051745898190997No Hit
GCTCTGGTCCGTCTTGCGCCGGTCC50.1051745898190997No Hit
GTACAGGAGGGCTGGGAGAATCTAT50.1051745898190997No Hit
CTAAGAAGTTGGGGGACGCCGACCG50.1051745898190997No Hit
GGAATGGAGGGATGGAGGTCAGATG50.1051745898190997No Hit
CTAGTTAGCATGCCAGAGTCTCGTT50.1051745898190997No Hit
CCACTCCTGGTGGTGCCCTTCCGTC50.1051745898190997No Hit
GAGCGAAAGCATTTGCCAAGAATGT50.1051745898190997No Hit
GTGTGCACATGCACCTAGAACCTTA50.1051745898190997No Hit
CCAGAGTCTCGTTCGTTATCGGAAT50.1051745898190997No Hit
CCTCAAAGCTGGCAGTCATCACTGC50.1051745898190997No Hit
GAATAGAGGCCATAGGTCAGTCTAA50.1051745898190997No Hit
ACGCCGGCAGGCGCGGGTAACCCGT50.1051745898190997No Hit
GTATGGTTGCAAAGCTGAAACTTAA50.1051745898190997No Hit
CAACCATACTCCCCCCGGAACCCAA50.1051745898190997No Hit
GTACATGGGAGAAAGGTAAATAAAG50.1051745898190997No Hit
GCTTTTAAAAGACAAATCCCTTGAG50.1051745898190997No Hit
CTGCTGAGATCAGAGCAGAAAGATC50.1051745898190997No Hit
GTATATAATAGTACGTGCAGTGCTT50.1051745898190997No Hit
GCTTTGAGGAATGGGGATTCCACAC50.1051745898190997No Hit
GAGTACATGGGCAGCTAGGAATAAT50.1051745898190997No Hit
CTCTTGGGTATCCATCCATCCATCT50.1051745898190997No Hit
ACTTAAAGGAATTGACGGAAGGGCA50.1051745898190997No Hit
GGTGAGGGGTGGGGTGCTTTGAGAT50.1051745898190997No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers