FastQCFastQC Report
Thu 2 Feb 2017
SRR4063927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4754
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT260.5469078670593185No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT210.44173327724021877No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA160.33655868742111905No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG160.33655868742111905No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC150.3155237694572991No Hit
GTACATGGGGAATGCACACCCATCAAAATAAAATCATGGGCTCAGCAGGA140.2944888514934792No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA140.2944888514934792No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT130.27345393352965924No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT130.27345393352965924No Hit
ACTCTCTACTGTGCATTTTGAGACAAGGTTTCTCATGGAACTTGGCTTGC130.27345393352965924No Hit
GTATCAACGCAGAGTACATGGGCAGCTAGGAATAATGGAATAGGACCGCG120.2524190155658393No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG120.2524190155658393No Hit
GTATTAGGGCAGCCTTTTACACTTTACAGTTTTCTAAGAGGCACCCGGAT120.2524190155658393No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC120.2524190155658393No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT120.2524190155658393No Hit
CCCATGTCCCCCTGGTATTCAGGAGAGAGACGACAGCCATCCCGTCTGAC120.2524190155658393No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG120.2524190155658393No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA110.23138409760201936No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAA110.23138409760201936No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA110.23138409760201936No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG100.2103491796381994No Hit
CTCTTCTAGAGCTCAAGGGGGTTTTGCTTTTCCCCACTGAGAGCTTGCTA100.2103491796381994No Hit
GTCCCAAAGTGCATCAGCGGCTCCTGAATCTGCGAGCCTTGGGAGAACAA100.2103491796381994No Hit
ATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGTCAAATTAAG100.2103491796381994No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA100.2103491796381994No Hit
GTACATGGGCATACCCATGTCCCCCTGGTATTCAGGAGAGAGACGACAGC100.2103491796381994No Hit
CCCCAGAGCAGCGCAAGAGAAAGAAAGGAAGGAAAACAAAACCACCACGG100.2103491796381994No Hit
GGAGAGAGACGACAGCCATCCCGTCTGACTTTTAGGTTTCACCTTTGGAT90.18931426167437948No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACAACACGGGAAACCTCACC90.18931426167437948No Hit
GTCCCAGAGTTCCCTGGGCTTGCAAGCCAAGTTCCATGAGAAACCTTGTC90.18931426167437948No Hit
GCACAGAACTTGCCGGGTTGCTTTTTAGTTTCAAGCCCAGAGGACGAAAC90.18931426167437948No Hit
CCTTTGGATTATCAGTTAAATCAAATGCAGGGCTGTTACTGCCTACTGTG90.18931426167437948No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC90.18931426167437948No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC90.18931426167437948No Hit
GCTCAGAGGTGGATAGCAGTTGGTTTAGTGAGAAAGGTGTCAGAGGCAGC90.18931426167437948No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG90.18931426167437948No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA80.16827934371055953No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC80.16827934371055953No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACAACACGGGAAACCTCACCCGGCC80.16827934371055953No Hit
GTCTAAAGATGTCTCCCTCTATCACTCTCTACTGTGCATTTTGAGACAAG80.16827934371055953No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT80.16827934371055953No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC80.16827934371055953No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT80.16827934371055953Illumina PCR Primer Index 10 (95% over 21bp)
ACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG80.16827934371055953No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG80.16827934371055953No Hit
ATGCAGGGCTGTTACTGCCTACTGTGCAAGCGTGAAGACAGGAGATTGGA80.16827934371055953No Hit
CTTTGGGACTTAGCAAGCTCTCAGTGGGGAAAAGCAAAACCCCCTTGAGC80.16827934371055953No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC80.16827934371055953No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGG80.16827934371055953No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG80.16827934371055953No Hit
CTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGT70.1472444257467396No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA70.1472444257467396No Hit
CCCCTCACCCCTGCTGCCTCTGACACCTTTCTCACTAAACCAACTGCTAT70.1472444257467396No Hit
GCTCTGGGGAGGAGGCTCCTGGGGAGTCTGCATTTGTCTCTTGCACTTGC70.1472444257467396No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGTCAAAT70.1472444257467396No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT70.1472444257467396No Hit
GGTAAATAAAGCGTGCATAATGAGGTTAATTTAAAGGGTGCCAGAAAAGT70.1472444257467396No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC70.1472444257467396No Hit
ACCCAAGAGAGTGTGAAAGCAATTGGAACAGGAATGGAGGGATGGAGGTC70.1472444257467396No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA70.1472444257467396No Hit
CCTTAGATCCACATGAGAAAAGGCCTAAGCAGCTTCCAGTCTGGAGCAGC70.1472444257467396No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG70.1472444257467396No Hit
CAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCC70.1472444257467396No Hit
AGCTAGACCAGCATAACTATTGAGAATATCAGCACCAGGAACATGTTCCA60.12620950778291964No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC60.12620950778291964No Hit
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGTCAAATTAAGCCGCAGGCTC60.12620950778291964No Hit
GGTCTGAAGGACTCCTTTCACCGAAGAGCGTCTTGTCTGCAGTAACTTCA60.12620950778291964No Hit
GAATGTGTAGTGTGCACATGCACCTAGAACCTTAGTCCTGGAGGAGTAGA60.12620950778291964No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGC60.12620950778291964No Hit
GGCCATAGGTCAGTCTAAAGATGTCTCCCTCTATCACTCTCTACTGTGCA60.12620950778291964No Hit
CCTCTGAAGATGACATCGTTGCCCCAATCACCCATGTGTCCGTCACATTA60.12620950778291964No Hit
ATCCAAAGGTGAAACCTAAAAGTCAGACGGGATGGCTGTCGTCTCTCTCC60.12620950778291964No Hit
ACCTAGTGTTTGAATTTGCAAGTAACGGCATGGAGGACGAGCAGCAGCTT60.12620950778291964No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA60.12620950778291964No Hit
CCCTAATACTGGCAGCAACCATCTACAAAAATGTTAAAAATAAAAAAAAT60.12620950778291964No Hit
GGTTTCCCGTGTTGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCC60.12620950778291964No Hit
CCACCAGGAGTGGAGCCTGCGGCTTAATTTGACAACACGGGAAACCTCAC60.12620950778291964No Hit
GTACATGGGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTT60.12620950778291964No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA60.12620950778291964No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACT60.12620950778291964No Hit
CCTCTGAGCTGTGGAGCTGCTTTTAAAAGACAAATCCCTTGAGTCCTCAA60.12620950778291964No Hit
GGTATCAACGCAGAGTACATGGGCAGCTAGGAATAATGGAATAGGACCGC60.12620950778291964No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG60.12620950778291964No Hit
CTCTCTACTGTGCATTTTGAGACAAGGTTTCTCATGGAACTTGGCTTGCA60.12620950778291964No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT60.12620950778291964No Hit
ACCCCCTTCTGCTCTGAATTCTTGCTCCTTGTTCTCCCAAGGCTCGCAGA60.12620950778291964No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT60.12620950778291964No Hit
CACTACACATTCTCTTTTCACACAAGTACTAGGGATCCAATCTCCTGTCT60.12620950778291964No Hit
TGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGTCAAA60.12620950778291964No Hit
TCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAG50.1051745898190997No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC50.1051745898190997No Hit
GACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG50.1051745898190997No Hit
GCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTG50.1051745898190997No Hit
GTGTGGAATCCCCATTCCTCAAAGCTGGCAGTCATCACTGCTGAGATCAG50.1051745898190997No Hit
GTCCTGGAGGAGTAGAGACAGGCAGAGTCCCAGAGTTCCCTGGGCTTGCA50.1051745898190997No Hit
CTCCCAAGGCTCGCAGATTCAGGAGCCGCTGATGCACTTTGGGACTTAGC50.1051745898190997No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG50.1051745898190997No Hit
CCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC50.1051745898190997No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAA50.1051745898190997No Hit
GGCCTAAGCAGCTTCCAGTCTGGAGCAGCAGGTAGGGTGCAGTGGAGTTT50.1051745898190997No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG50.1051745898190997No Hit
TAGTAAAGCATTCCCCTTAGATCCACATGAGAAAAGGCCTAAGCAGCTTC50.1051745898190997No Hit
GTGCATCAGCGGCTCCTGAATCTGCGAGCCTTGGGAGAACAAGGAGCAAG50.1051745898190997No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC50.1051745898190997No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG50.1051745898190997No Hit
ACCAACTGCTATCCACCTCTGAGCTGTGGAGCTGCTTTTAAAAGACAAAT50.1051745898190997No Hit
TATCAACGCAGAGTACATGGGCAGCTAGGAATAATGGAATAGGACCGCGG50.1051745898190997No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCCCATGTACTCTG50.1051745898190997No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGG50.1051745898190997No Hit
GTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGTCAAATTAAGCCGCA50.1051745898190997No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA50.1051745898190997No Hit
GTATCAACGCAGAGTACATGGGGGCAGGAGCCATTTTAACAGCACAGAAC50.1051745898190997No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG50.1051745898190997No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT50.1051745898190997No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.1051745898190997Illumina PCR Primer Index 10 (95% over 23bp)
ATATTGAGGAGTGCCTCGGCAGCCTCAATGGTCTCAATTGTTTCATCTCC50.1051745898190997No Hit
ACGCAGAGTACATGGGAATAGAGGCCATAGGTCAGTCTAAAGATGTCTCC50.1051745898190997No Hit
ACGCAGAGTACATGGGGAATGCACACCCATCAAAATAAAATCATGGGCTC50.1051745898190997No Hit
CTACTGTGCAAGCGTGAAGACAGGAGATTGGATCCCTAGTACTTGTGTGA50.1051745898190997No Hit
ATTATATACACTGCTGATTTATGTGGTTGGATACCCTGGTTGAATTTTTT50.1051745898190997No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC50.1051745898190997No Hit
CTTCAGACCTGCTTGCTGCATTGACATTCTAAGAAAACAGGCACGTTTTC50.1051745898190997No Hit
GATCTCAGCAGTGATGACTGCCAGCTTTGAGGAATGGGGATTCCACACAG50.1051745898190997No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT50.1051745898190997No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC50.1051745898190997No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA50.1051745898190997No Hit
CTCTCAGGCAGGTCTGTGAAGTTACTGCAGACAAGACGCTCTTCGGTGAA50.1051745898190997No Hit
ATTTAAAGGGTGCCAGAAAAGTCTTTCTTAAAATAGGTCACTGCAGAAAA50.1051745898190997No Hit
GTATCAACGCAGAGTACATGGGAGAAAGGTAAATAAAGCGTGCATAATGA50.1051745898190997No Hit
CCCATGTGTCCGTCACATTAGATGGGATTCCTGAAGTGATGGAAACTCAG50.1051745898190997No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA50.1051745898190997No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA50.1051745898190997No Hit
GGAATGGAGGGATGGAGGTCAGATGGACGGACACACGAGGGAGTGAGAAA50.1051745898190997No Hit
GCCCTAATACTGGCAGCAACCATCTACAAAAATGTTAAAAATAAAAAAAA50.1051745898190997No Hit
CTATCACTCTCTACTGTGCATTTTGAGACAAGGTTTCTCATGGAACTTGG50.1051745898190997No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACC50.1051745898190997No Hit
AATGTGTAGTGTGCACATGCACCTAGAACCTTAGTCCTGGAGGAGTAGAG50.1051745898190997No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC50.1051745898190997No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC50.1051745898190997No Hit
GCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAA50.1051745898190997No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAA50.1051745898190997No Hit
CTTCAGGAATCCCATCTAATGTGACGGACACATGGGTGATTGGGGCAACG50.1051745898190997No Hit
GATCTAAGGGGAATGCTTTACTATGGCTGCTGTTGTCCAACAGAACGACC50.1051745898190997No Hit
GACCTAGTGTTTGAATTTGCAAGTAACGGCATGGAGGACGAGCAGCAGCT50.1051745898190997No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers