FastQCFastQC Report
Thu 2 Feb 2017
SRR4063926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289427
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT26670.11649203053864568No Hit
GTCCTAAAGTGTGTATTTCTCATTT25970.1134344969287075No Hit
GTATCAACGCAGAGTACTTTTTTTT24230.10583434195543251No Hit
CTGTAGGACGTGGAATATGGCAAGA23110.1009422881795314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTGC350.002170287516.2860898
GGTATCA14900.015.6835541
GTATCAA46950.013.0705111
TCCAACG1251.4078978E-912.16187518
CCAACGT752.0712423E-411.40200719
GGCGAGG5350.011.36648719
CCAACGA600.005869403511.08528419
AATCCCG1900.011.00193619
GTCCTAA8500.010.9522361
GTCCTAG3950.010.8220871
TAGGACC5450.010.6311814
GGCGTGC909.516446E-510.5557998
GCCGGTT1002.3997016E-510.45001111
GTATTAG3300.010.3629681
AGGACGT14050.010.3438515
GGACGTG13950.010.14636
TGGCGAG12550.010.1449918
TGTCGAG750.002645003810.13489518
TGTAGGA17100.010.0524912
GACGTGG7350.09.8229267
Produced by FastQC (version 0.11.5)