##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063926_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2289427
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.353629095839263	32.0	32.0	32.0	32.0	32.0
2	31.06243483631494	32.0	32.0	32.0	32.0	32.0
3	31.0776299047753	32.0	32.0	32.0	32.0	32.0
4	31.09395014560412	32.0	32.0	32.0	32.0	32.0
5	31.083399470697252	32.0	32.0	32.0	32.0	32.0
6	34.74804437966356	36.0	36.0	36.0	32.0	36.0
7	34.68345529252516	36.0	36.0	36.0	32.0	36.0
8	34.665703689176375	36.0	36.0	36.0	32.0	36.0
9	34.73711326021751	36.0	36.0	36.0	32.0	36.0
10	34.54607026124877	36.0	36.0	36.0	32.0	36.0
11	34.73519706022511	36.0	36.0	36.0	32.0	36.0
12	34.60682913235495	36.0	36.0	36.0	32.0	36.0
13	34.64621278599405	36.0	36.0	36.0	32.0	36.0
14	34.58144679869679	36.0	36.0	36.0	32.0	36.0
15	34.55533284092483	36.0	36.0	36.0	32.0	36.0
16	34.55278547863723	36.0	36.0	36.0	32.0	36.0
17	34.522881052769975	36.0	36.0	36.0	32.0	36.0
18	34.50420913180459	36.0	36.0	36.0	32.0	36.0
19	34.499705821587675	36.0	36.0	36.0	32.0	36.0
20	34.47764833733506	36.0	36.0	36.0	32.0	36.0
21	34.46620529940461	36.0	36.0	36.0	32.0	36.0
22	34.44601553139716	36.0	36.0	36.0	32.0	36.0
23	34.40522366513542	36.0	36.0	36.0	32.0	36.0
24	34.404751057797434	36.0	36.0	36.0	32.0	36.0
25	34.02728324598251	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	14.0
5	20.0
6	80.0
7	32.0
8	126.0
9	137.0
10	211.0
11	54.0
12	152.0
13	129.0
14	171.0
15	339.0
16	645.0
17	764.0
18	847.0
19	1022.0
20	1586.0
21	2255.0
22	3364.0
23	5499.0
24	8435.0
25	12802.0
26	18934.0
27	25997.0
28	36329.0
29	50596.0
30	67139.0
31	93446.0
32	131886.0
33	191559.0
34	441115.0
35	1193733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51023689827772	19.4673011336947	12.24451450382013	24.77794746420745
2	14.081380461717687	21.819220633541562	42.06212234981464	22.03727655492611
3	17.915288388920896	26.744929109968545	30.034013412300474	25.30576908881008
4	11.011238114540708	17.918607536106286	39.7935747757444	31.276579573608604
5	11.984934797394255	39.449400218150146	35.729009819377445	12.836655165078154
6	29.069074080303135	39.51368869943486	19.69704051851538	11.72019670174663
7	27.498757054643285	33.195815664868725	22.380574577158292	16.924852703329694
8	25.16469366345035	36.939094476508565	20.985011642270113	16.911200217770965
9	27.358368122486954	15.612052393273808	20.613999245731236	36.415580238508
10	13.724858627924958	30.730371035321273	35.02640387638537	20.5183664603684
11	33.31325938311498	23.95418069876576	24.70822120036395	18.024338717755306
12	22.890999134174915	26.80884671312081	31.89212719805279	18.408026954651483
13	28.23247009418545	23.38315851032351	26.397495031541567	21.986876363949467
14	21.01622018541101	22.81796764704597	28.9259098995144	27.239902268028622
15	22.40199958305599	31.546578196450557	24.41985125500584	21.63157096548761
16	21.222254808845943	29.966969984514314	27.85483227334534	20.955942933294406
17	20.767230049697925	29.62990319590319	28.03949086176144	21.563375892637442
18	20.425428864677915	29.314874361139815	30.011435225740797	20.24826154844147
19	22.666790805508093	28.005766336605966	28.614090370453905	20.713352487432036
20	23.70040413268841	27.833136257630763	28.64170762999893	19.824751979681896
21	23.944982561171408	27.511516970456494	27.18402805249439	21.359472415877708
22	22.981541311626042	28.862382297284245	27.726237722657626	20.42983866843209
23	22.12006506544358	28.697515194249902	27.87597872477151	21.306441015535007
24	21.892960366765223	28.287921891037016	28.807182485631706	21.01193525656605
25	21.93002740346388	28.486444924020848	28.373797410199998	21.209730262315276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	79.0
2	306.0
3	533.0
4	533.0
5	533.0
6	1294.0
7	2055.0
8	2055.0
9	2055.0
10	3463.5
11	4872.0
12	4872.0
13	4872.0
14	8154.0
15	11436.0
16	11436.0
17	11436.0
18	19703.5
19	27971.0
20	27971.0
21	27971.0
22	43537.5
23	59104.0
24	59104.0
25	59104.0
26	85826.0
27	112548.0
28	112548.0
29	112548.0
30	145643.0
31	178738.0
32	178738.0
33	178738.0
34	210298.5
35	241859.0
36	241859.0
37	241859.0
38	266514.0
39	291169.0
40	291169.0
41	291169.0
42	307822.0
43	324475.0
44	324475.0
45	324475.0
46	330266.0
47	336057.0
48	336057.0
49	336057.0
50	316659.5
51	297262.0
52	297262.0
53	297262.0
54	249125.0
55	200988.0
56	200988.0
57	200988.0
58	156939.0
59	112890.0
60	112890.0
61	112890.0
62	83539.5
63	54189.0
64	54189.0
65	54189.0
66	37828.0
67	21467.0
68	21467.0
69	21467.0
70	14248.0
71	7029.0
72	7029.0
73	7029.0
74	4649.5
75	2270.0
76	2270.0
77	2270.0
78	1495.0
79	720.0
80	720.0
81	720.0
82	548.5
83	377.0
84	377.0
85	377.0
86	286.5
87	196.0
88	196.0
89	196.0
90	164.0
91	132.0
92	132.0
93	132.0
94	127.5
95	123.0
96	123.0
97	123.0
98	505.5
99	888.0
100	888.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03896171400092687
2	0.0046299794664778566
3	0.0015724458565396495
4	0.0032759288677909363
5	0.007774871179557156
6	0.012055418233470646
7	0.02223263724940782
8	0.03424437643130792
9	0.04883317965587023
10	0.057481631866838294
11	0.053332121967636446
12	0.0627231180553038
13	0.06525650304639545
14	0.06713470226392892
15	0.058966719620236856
16	0.06586800976838308
17	0.061325388405046324
18	0.0768314517125901
19	0.07276929991652933
20	0.07914644144582902
21	0.07495325249505662
22	0.07766135369243046
23	0.08229133315890833
24	0.07648201930002572
25	0.07687513076416064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2289427.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.565636365249475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51462574735824	47.3656960414923
2	16.104060930603097	20.473297622370755
3	5.155656477938832	9.831677547024078
4	1.9749454083983393	5.021546466858719
5	0.9059013554330556	2.879209807112212
6	0.4350031447888966	1.6590751031634585
7	0.24072518545024726	1.0711294721601363
8	0.14401133280574926	0.7323337610884145
9	0.09527116821256305	0.5450375191223069
>10	0.3779861162873248	4.251595153597408
>50	0.029095366832970555	1.2825973217252455
>100	0.01870491495279897	2.3355701571319196
>500	0.002698296320291815	1.1887140927418969
>1k	0.0013145546175762	1.3625199344111047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	2667	0.11649203053864568	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2597	0.1134344969287075	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2423	0.10583434195543251	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2311	0.1009422881795314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.367905157054582E-5	0.0
4	0.0	0.0	0.0	4.367905157054582E-5	0.0
5	0.0	0.0	0.0	4.367905157054582E-5	0.0
6	0.0	0.0	0.0	8.735810314109164E-5	0.0
7	0.0	0.0	0.0	8.735810314109164E-5	0.0
8	0.0	0.0	0.0	8.735810314109164E-5	0.0
9	0.0	0.0	0.0	8.735810314109164E-5	0.0
10	0.0	0.0	0.0	8.735810314109164E-5	0.0
11	0.0	0.0	0.0	1.7471620628218328E-4	0.0
12	0.0	0.0	0.0	1.7471620628218328E-4	0.0
13	0.0	0.0	0.0	1.7471620628218328E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTGC	35	0.0021702875	16.286089	8
GGTATCA	1490	0.0	15.683554	1
GTATCAA	4695	0.0	13.070511	1
TCCAACG	125	1.4078978E-9	12.161875	18
CCAACGT	75	2.0712423E-4	11.402007	19
GGCGAGG	535	0.0	11.366487	19
CCAACGA	60	0.0058694035	11.085284	19
AATCCCG	190	0.0	11.001936	19
GTCCTAA	850	0.0	10.952236	1
GTCCTAG	395	0.0	10.822087	1
TAGGACC	545	0.0	10.631181	4
GGCGTGC	90	9.516446E-5	10.555799	8
GCCGGTT	100	2.3997016E-5	10.450011	11
GTATTAG	330	0.0	10.362968	1
AGGACGT	1405	0.0	10.343851	5
GGACGTG	1395	0.0	10.1463	6
TGGCGAG	1255	0.0	10.14499	18
TGTCGAG	75	0.0026450038	10.134895	18
TGTAGGA	1710	0.0	10.052491	2
GACGTGG	735	0.0	9.822926	7
>>END_MODULE