FastQCFastQC Report
Thu 2 Feb 2017
SRR4063926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289427
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCG301.3017915E-436.6652647
GTATCAA44200.029.2173541
GGTATCA15700.028.5861421
CGGCCGC752.0659045E-623.46628439
ACGGCCG752.0659045E-623.46628438
CGCGGTA752.0662974E-623.4657743
GGCCGCG803.6183992E-621.9996440
CCGCGGT803.619085E-621.99915742
GTAGGAC21050.020.9018153
TAGGACC9000.020.7769834
TAGGACG18150.020.6052724
AGGACGT18450.020.0317545
TATCAAC64400.020.0178682
GGACGTG18250.019.889656
ATCAACG65400.019.644513
TCAACGC66200.019.4071124
TACCGCG808.995687E-519.2492628
GACGTGG9450.018.8564227
CAACGCA68450.018.8334665
GCCGCGG951.5983525E-518.5260141