##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2289427
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.354247591209504	32.0	32.0	32.0	32.0	32.0
2	31.484516868194532	32.0	32.0	32.0	32.0	32.0
3	31.539648130296356	32.0	32.0	32.0	32.0	32.0
4	31.60796959239146	32.0	32.0	32.0	32.0	32.0
5	31.596631820975293	32.0	32.0	32.0	32.0	32.0
6	35.18927618133271	36.0	36.0	36.0	36.0	36.0
7	35.20259130341348	36.0	36.0	36.0	36.0	36.0
8	35.153159720751084	36.0	36.0	36.0	36.0	36.0
9	35.24378720090223	36.0	36.0	36.0	36.0	36.0
10	35.144167077613744	36.0	36.0	36.0	36.0	36.0
11	35.25426100067833	36.0	36.0	36.0	36.0	36.0
12	35.184592913423316	36.0	36.0	36.0	36.0	36.0
13	35.215351264748776	36.0	36.0	36.0	36.0	36.0
14	35.17075407951422	36.0	36.0	36.0	36.0	36.0
15	35.160753760657144	36.0	36.0	36.0	36.0	36.0
16	35.17486646221958	36.0	36.0	36.0	36.0	36.0
17	35.15966003720582	36.0	36.0	36.0	36.0	36.0
18	35.15029961645425	36.0	36.0	36.0	36.0	36.0
19	35.14305457217024	36.0	36.0	36.0	36.0	36.0
20	35.13775674000525	36.0	36.0	36.0	36.0	36.0
21	35.13485426702839	36.0	36.0	36.0	36.0	36.0
22	35.120491284500446	36.0	36.0	36.0	36.0	36.0
23	35.083022520482196	36.0	36.0	36.0	36.0	36.0
24	35.07188261516965	36.0	36.0	36.0	36.0	36.0
25	35.042104858551944	36.0	36.0	36.0	36.0	36.0
26	34.996807934911224	36.0	36.0	36.0	36.0	36.0
27	34.988595836425446	36.0	36.0	36.0	36.0	36.0
28	34.97133300166374	36.0	36.0	36.0	36.0	36.0
29	34.95270912765508	36.0	36.0	36.0	36.0	36.0
30	34.94281232815023	36.0	36.0	36.0	36.0	36.0
31	34.9230571667059	36.0	36.0	36.0	36.0	36.0
32	34.9058502411302	36.0	36.0	36.0	36.0	36.0
33	34.896527821153505	36.0	36.0	36.0	36.0	36.0
34	34.891143941256914	36.0	36.0	36.0	36.0	36.0
35	34.86744063034113	36.0	36.0	36.0	36.0	36.0
36	34.8476326172444	36.0	36.0	36.0	36.0	36.0
37	34.82537595651663	36.0	36.0	36.0	36.0	36.0
38	34.81389972250699	36.0	36.0	36.0	36.0	36.0
39	34.78918000006115	36.0	36.0	36.0	36.0	36.0
40	34.78880654417022	36.0	36.0	36.0	36.0	36.0
41	34.76942833294095	36.0	36.0	36.0	32.0	36.0
42	34.75460104209481	36.0	36.0	36.0	32.0	36.0
43	34.73035829489213	36.0	36.0	36.0	32.0	36.0
44	34.70471912841073	36.0	36.0	36.0	32.0	36.0
45	34.68192434176761	36.0	36.0	36.0	32.0	36.0
46	34.659024288610205	36.0	36.0	36.0	32.0	36.0
47	34.6367527770049	36.0	36.0	36.0	32.0	36.0
48	34.61364088044738	36.0	36.0	36.0	32.0	36.0
49	34.593174624043485	36.0	36.0	36.0	32.0	36.0
50	34.07279856488108	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	2.0
20	17.0
21	52.0
22	180.0
23	550.0
24	1306.0
25	3176.0
26	6437.0
27	12198.0
28	20977.0
29	33109.0
30	48426.0
31	69300.0
32	99406.0
33	157343.0
34	335032.0
35	1501913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68271656441182	20.357240364054928	12.71195584512595	25.248087226407307
2	13.65942828189734	22.553883852425184	41.331519187215946	22.45516867846153
3	17.20290099422474	27.184678054111473	30.321364955870955	25.291055995792828
4	10.809822719833392	18.6689944689217	39.521067935339275	31.00011487590563
5	11.612075860029606	39.9555434613115	35.4921558975237	12.940224781135193
6	29.306973247821173	39.80700797275818	19.29597046589509	11.59004831352556
7	27.46644023406771	33.24086461960066	22.371007891508036	16.921687254823595
8	25.21648429934652	37.2257337753071	20.718983396282127	16.83879852906426
9	27.216626605066587	15.536271436377621	20.502745053160425	36.744356905395364
10	13.588423577757084	31.016260943858075	34.87222920120974	20.5230862771751
11	33.349581947614816	23.968758981508902	24.680334721454198	18.00132434942208
12	22.860781952483226	26.9314145966036	31.81124676392261	18.396556686990564
13	28.282172654133948	23.25963638841436	26.439130242974095	22.01906071447759
14	21.11812501146578	22.850308942606475	28.80801355101855	27.2235524949092
15	22.43225877186277	31.64116947395687	24.311711207218508	21.61486054696185
16	21.35870296374078	30.023927624831614	27.653004886861382	20.96436452456622
17	20.759648593294305	29.787234971894716	27.863303787366885	21.5898126474441
18	20.48340543298227	29.411721026773545	29.891579716120738	20.21329382412345
19	22.751903752294243	28.061182147839673	28.567903046440463	20.619011053425616
20	23.810470194761717	27.987348722139693	28.483720499534158	19.718460583564436
21	23.97368424500978	27.529770549574195	27.14779724358977	21.348747961826255
22	23.125766571038202	28.87216652631064	27.603589736421863	20.39847716622929
23	22.18620613442159	28.86513506324121	27.702302209871632	21.246356592465567
24	22.01048476075579	28.34196289187907	28.62247086377411	21.025081483591027
25	21.979638137520322	28.542556100390588	28.226356678538888	21.251449083550206
26	21.099055567373764	29.064902060052127	28.63062292541293	21.205419447161177
27	21.572183636919583	29.007207443323285	28.135543615952475	21.285065303804657
28	20.967697550070305	29.114007541941294	28.146082851278386	21.772212056710018
29	21.633378687976798	28.742249488395256	27.929054563164897	21.695317260463053
30	21.34578407245401	28.720173744246164	28.441709451886176	21.492332731413654
31	22.14025928187709	28.297046276372225	28.168925489003804	21.393768952746886
32	21.272193342357653	28.9661037604685	28.376814746770833	21.384888150403015
33	21.328142881853747	28.87195915664482	28.56470795847872	21.235190003022716
34	21.416397085995285	28.981487386420124	28.82624735842974	20.775868169154847
35	21.526955432386355	28.984739356490675	28.45611838803365	21.03218682308932
36	20.99789275411446	28.76740094109671	29.118171763254598	21.116534541534236
37	21.311469681218167	28.44929106205282	28.069399914385805	22.16983934234321
38	21.025849581783334	29.015779881917346	28.391818434382703	21.566552101916614
39	21.828183893807086	28.241290186747904	28.4294227645224	21.501103154922614
40	22.043550883912065	28.037170083621334	28.885308085026086	21.033970947440515
41	21.979474478508045	27.790175792639378	28.953303237388862	21.277046491463718
42	21.408144821678874	28.410561632161706	28.907551866639935	21.273741679519485
43	20.91687465547531	28.44727800379665	29.0041958848355	21.631651455892538
44	20.673984744467873	28.467820593963744	29.030282620023858	21.82791204154453
45	20.875420581281222	28.465648724922914	28.864881533997472	21.794049159798394
46	20.898461301920197	28.050282104111858	28.915660545565807	22.135596048402135
47	21.393811504177773	28.045839544994116	28.810327001625335	21.750021949202775
48	20.52385247566486	28.21469521290652	29.155166125400488	22.106286186028125
49	21.239328073772462	27.906612073461467	28.897344215866216	21.956715636899858
50	20.598708130878187	28.684596286531473	28.788684875836246	21.928010706754094
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	23.0
4	44.0
5	74.5
6	105.0
7	143.0
8	181.0
9	271.0
10	361.0
11	578.5
12	796.0
13	1371.0
14	1946.0
15	2762.0
16	3578.0
17	4230.5
18	4883.0
19	5930.0
20	6977.0
21	8996.0
22	11015.0
23	14103.0
24	17191.0
25	22167.0
26	27143.0
27	34547.5
28	41952.0
29	51835.0
30	61718.0
31	74032.0
32	86346.0
33	101141.0
34	115936.0
35	132721.0
36	149506.0
37	160285.5
38	171065.0
39	176314.5
40	181564.0
41	186600.0
42	191636.0
43	193819.0
44	196002.0
45	195634.5
46	195267.0
47	191201.0
48	187135.0
49	180061.5
50	172988.0
51	159749.5
52	146511.0
53	131073.5
54	115636.0
55	99014.5
56	82393.0
57	68144.5
58	53896.0
59	42946.0
60	31996.0
61	24496.0
62	16996.0
63	12567.5
64	8139.0
65	6182.0
66	4225.0
67	3113.5
68	2002.0
69	1532.5
70	1063.0
71	728.0
72	393.0
73	311.0
74	229.0
75	201.5
76	174.0
77	154.5
78	135.0
79	114.5
80	94.0
81	79.0
82	64.0
83	59.5
84	55.0
85	41.5
86	28.0
87	22.5
88	17.0
89	15.5
90	14.0
91	12.5
92	11.0
93	10.0
94	9.0
95	6.0
96	3.0
97	4.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009259958932955713
2	5.241486188465498E-4
3	2.183952578527291E-4
4	0.0
5	0.0
6	2.620743094232749E-4
7	1.7471620628218326E-4
8	0.0
9	0.0015724458565396495
10	6.551857735581872E-4
11	4.3679051570545814E-5
12	1.7471620628218326E-4
13	2.620743094232749E-4
14	2.183952578527291E-4
15	0.003931114641349124
16	0.0010046181861225539
17	0.0
18	1.3103715471163746E-4
19	4.3679051570545814E-5
20	6.115067219876415E-4
21	0.0
22	4.8046956727600397E-4
23	7.862229282698248E-4
24	3.931114641349124E-4
25	0.002140273526956745
26	0.005066769982183315
27	0.005547239549459319
28	0.004193188950772399
29	0.002708101197373841
30	0.003712719383496395
31	0.007905908334268793
32	0.0025333849910916576
33	0.004149509899201853
34	0.0030138545583676614
35	0.0032322498162203903
36	0.007993266437409885
37	0.003800077486637486
38	0.007993266437409885
39	0.0036690403319258485
40	0.002577064042662203
41	0.0035816822287847573
42	0.0020092363722451077
43	0.0017908411143923786
44	0.001223013443975283
45	0.0015724458565396495
46	0.0010482972376930997
47	0.002183952578527291
48	0.0018345201659629243
49	3.931114641349124E-4
50	0.0013540505986869204
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2289427.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.07428743233177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.76718558523066	53.79405828070609
2	15.05203660975	21.09521479667204
3	4.658148578001129	9.792493270720758
4	1.7792706402337994	4.98724489054609
5	0.7690038030338586	2.694369676517543
6	0.35623484128815364	1.4977741597102299
7	0.19424428596507565	0.9528070948763332
8	0.10855161293976713	0.6085341541107574
9	0.06544889007963947	0.41276559020109704
>10	0.23200481923240854	2.732534308318117
>50	0.012896300363456466	0.6038932362634529
>100	0.004534128248959985	0.570912825319666
>500	3.1421830935842815E-4	0.15505515109853815
>1k	1.2568732374280264E-4	0.10234256493943387
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.367905157054582E-5	0.0
3	0.0	0.0	0.0	4.367905157054582E-5	0.0
4	0.0	0.0	0.0	8.735810314109164E-5	0.0
5	0.0	0.0	0.0	8.735810314109164E-5	0.0
6	0.0	0.0	0.0	8.735810314109164E-5	0.0
7	0.0	0.0	0.0	1.3103715471163746E-4	0.0
8	0.0	0.0	0.0	1.7471620628218328E-4	0.0
9	0.0	0.0	0.0	2.1839525785272908E-4	0.0
10	0.0	0.0	0.0	2.620743094232749E-4	0.0
11	0.0	0.0	0.0	3.0575336099382074E-4	0.0
12	0.0	0.0	0.0	3.4943241256436657E-4	0.0
13	0.0	0.0	0.0	5.241486188465498E-4	0.0
14	0.0	0.0	0.0	5.241486188465498E-4	0.0
15	0.0	0.0	0.0	5.678276704170957E-4	0.0
16	0.0	0.0	0.0	5.678276704170957E-4	0.0
17	0.0	0.0	0.0	6.551857735581873E-4	0.0
18	0.0	0.0	0.0	6.988648251287331E-4	0.0
19	0.0	0.0	0.0	7.42543876699279E-4	0.0
20	0.0	0.0	0.0	8.735810314109163E-4	0.0
21	0.0	0.0	0.0	9.172600829814621E-4	0.0
22	0.0	0.0	0.0	0.0013103715471163746	0.0
23	0.0	0.0	0.0	0.0017034830112512868	0.0
24	0.0	0.0	0.0	0.0024897059395211116	0.0
25	0.0	0.0	0.0	0.002708101197373841	0.0
26	0.0	0.0	0.0	0.0032759288677909363	0.0
27	0.0	0.0	0.0	0.0039747936929196695	0.0
28	0.0	0.0	0.0	0.007425438766992789	0.0
29	0.0	0.0	0.0	0.014239370811997937	0.0
30	0.0	0.0	0.0	0.03319607919361482	0.0
31	0.0	0.0	0.0	0.08425689047958289	0.0
32	0.0	0.0	0.0	0.14885820775242015	0.0
33	0.0	0.0	0.0	0.21664809578990726	0.0
34	0.0	0.0	0.0	0.29474623999804317	0.0
35	0.0	0.0	0.0	0.3813617992624355	0.0
36	0.0	0.0	0.0	0.49855269462621	0.0
37	0.0	0.0	0.0	0.6778988803748711	0.0
38	0.0	0.0	0.0	0.9236372245107619	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGGCG	30	1.3017915E-4	36.665264	7
GTATCAA	4420	0.0	29.217354	1
GGTATCA	1570	0.0	28.586142	1
CGGCCGC	75	2.0659045E-6	23.466284	39
ACGGCCG	75	2.0659045E-6	23.466284	38
CGCGGTA	75	2.0662974E-6	23.46577	43
GGCCGCG	80	3.6183992E-6	21.99964	40
CCGCGGT	80	3.619085E-6	21.999157	42
GTAGGAC	2105	0.0	20.901815	3
TAGGACC	900	0.0	20.776983	4
TAGGACG	1815	0.0	20.605272	4
AGGACGT	1845	0.0	20.031754	5
TATCAAC	6440	0.0	20.017868	2
GGACGTG	1825	0.0	19.88965	6
ATCAACG	6540	0.0	19.64451	3
TCAACGC	6620	0.0	19.407112	4
TACCGCG	80	8.995687E-5	19.249262	8
GACGTGG	945	0.0	18.856422	7
CAACGCA	6845	0.0	18.833466	5
GCCGCGG	95	1.5983525E-5	18.52601	41
>>END_MODULE