FastQCFastQC Report
Thu 2 Feb 2017
SRR4063925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767383
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58570.763243386939768No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43940.5725954314859725No Hit
TATCAACGCAGAGTACTTTTTTTTT43520.567122284439452No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28570.3723043121883075No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22940.2989380791599501No Hit
GGTATCAACGCAGAGTACTTTTTTT21020.27391797837585663No Hit
GTACATGGGGTGGTATCAACGCAAA17330.22583247218142702No Hit
GTATCAACGCAGAGTACATGGGGTG12190.1588515773740101No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11260.14673246605671483No Hit
GCGCAAGACGGACCAGAGCGAAAGC9190.11975766989886405No Hit
GTACATGGGTGGTATCAACGCAAAA8750.11402389680250931No Hit
GATTAAGAGGGACGGCCGGGGGCAT8510.11089638420449761No Hit
GAATAGGACCGCGGTTCTATTTTGT8420.10972356698024324No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8400.10946294093040893No Hit
GTATCTGATCGTCTTCGAACCTCCG8320.10842043673107171No Hit
TATCAACGCAGAGTACATGGGGTGG8180.10659605438223156No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8080.10529292413306002No Hit
ATCAGATACCGTCGTAGTTCCGACC7940.10346854178421988No Hit
ATCAACGCAGAGTACTTTTTTTTTT7930.10333822875930274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT701.3169483E-917.6342031
GTTATTC807.697963E-915.436973
CGTTATT755.8578735E-815.1945252
CACGCGC456.7552185E-414.778234512
ATCGCTC1006.548362E-1014.25229816
AAATCGC1006.566552E-1014.251368514
AAGCCCG400.00527707914.2495115
AATCGCT954.8748916E-914.00225815
CAAATCG954.8839865E-914.00043213
CGAACGA759.636769E-713.93558116
GTCCTAA1351.8189894E-1213.36381151
TCCTAAG655.463907E-513.1491082
CGCATCG1600.012.46913413
CAAGACG1600.012.4683224
GTTTTCG853.928866E-612.29610115
GCAAGAC1700.012.2936953
CGCAAGA1700.012.2896892
AACGAAC701.0885338E-412.2154614
TAACGAA701.0891968E-412.214663513
TAAATCC1405.2750693E-1112.2138674
Produced by FastQC (version 0.11.5)