##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063925_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767383
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168045161281917	32.0	32.0	32.0	32.0	32.0
2	30.998196467735145	32.0	32.0	32.0	32.0	32.0
3	31.025362824039625	32.0	32.0	32.0	32.0	32.0
4	31.005746804398846	32.0	32.0	32.0	32.0	32.0
5	31.0333471030763	32.0	32.0	32.0	32.0	32.0
6	34.62265127061715	36.0	36.0	36.0	32.0	36.0
7	34.52042721822089	36.0	36.0	36.0	32.0	36.0
8	34.46306081839186	36.0	36.0	36.0	32.0	36.0
9	34.49758464808316	36.0	36.0	36.0	32.0	36.0
10	34.39899111656109	36.0	36.0	36.0	32.0	36.0
11	34.565029457259286	36.0	36.0	36.0	32.0	36.0
12	34.44188495184282	36.0	36.0	36.0	32.0	36.0
13	34.50406250855179	36.0	36.0	36.0	32.0	36.0
14	34.42817211223079	36.0	36.0	36.0	32.0	36.0
15	34.409319726916024	36.0	36.0	36.0	32.0	36.0
16	34.40400165236915	36.0	36.0	36.0	32.0	36.0
17	34.325734346473666	36.0	36.0	36.0	32.0	36.0
18	34.3311814309152	36.0	36.0	36.0	32.0	36.0
19	34.31936725207621	36.0	36.0	36.0	32.0	36.0
20	34.26059477470832	36.0	36.0	36.0	32.0	36.0
21	34.22483818380131	36.0	36.0	36.0	32.0	36.0
22	34.22486424640629	36.0	36.0	36.0	32.0	36.0
23	34.20603792369651	36.0	36.0	36.0	32.0	36.0
24	34.18924578730569	36.0	36.0	36.0	32.0	36.0
25	33.77079111734297	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	8.0
5	9.0
6	29.0
7	12.0
8	30.0
9	42.0
10	79.0
11	31.0
12	44.0
13	48.0
14	122.0
15	212.0
16	316.0
17	467.0
18	645.0
19	758.0
20	1017.0
21	1341.0
22	2110.0
23	2754.0
24	3826.0
25	5068.0
26	7058.0
27	9038.0
28	12652.0
29	17145.0
30	22964.0
31	32498.0
32	47679.0
33	70093.0
34	162508.0
35	366776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.4707669083149	17.109529092300413	11.413088113175325	25.006615886209367
2	16.537192915238023	20.295515529682525	37.69543238498861	25.47185917009085
3	19.112417167840597	25.144097007291183	28.302958826634	27.440526998234215
4	12.341808031089094	15.400805115834771	35.403593959937076	36.85379289313905
5	14.254473705130996	37.00061382714054	32.902828948140076	15.842083519588385
6	33.33129152580367	35.343948994779055	17.294544203395308	14.030215276021968
7	29.43783261016622	30.739663750102643	21.069383637437163	18.753120002293976
8	28.524089239296785	31.744895751043533	19.86283512317724	19.868179886482444
9	27.860147117493213	13.734182001183823	19.015366242245825	39.39030463907714
10	16.3906339234723	26.685338319394784	30.93900680367905	25.985020953453862
11	36.84038281005528	21.290549702722437	22.650203400438095	19.218864086784187
12	25.333980103978814	23.933252226208033	28.80215729305621	21.93061037675694
13	29.86187085257227	19.44044199240831	25.835744584302073	24.861942570717353
14	23.97703370745492	19.85556742208191	25.975956591985792	30.191442278477382
15	25.038857130935494	27.002054994575648	23.77247976299758	24.186608111491278
16	24.803422726549602	25.752209619833245	25.02692750047596	24.417440153141193
17	23.141249155676334	25.434810389027835	26.211196970605798	25.21274348469003
18	23.982910708976053	24.43374769821439	28.134340815549375	23.449000777260185
19	24.974341519268965	24.34966999346638	27.02389009954317	23.652098387721487
20	25.5903211587066	23.48164937805333	26.55812703935648	24.369902423883588
21	26.98424497556739	23.359585186808086	25.803759244551728	23.852410593072797
22	25.588941053729787	23.708789775691184	26.366849243609806	24.335419926969223
23	23.956856582044878	23.85591037437479	27.125576299812842	25.061656743767486
24	24.18882402958836	24.821723866309853	26.85279373177462	24.13665837232716
25	24.342194493421946	24.28676877171384	26.865434172500496	24.50560256236372
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	349.0
1	349.0
2	711.0
3	1073.0
4	1073.0
5	1073.0
6	3068.5
7	5064.0
8	5064.0
9	5064.0
10	4530.5
11	3997.0
12	3997.0
13	3997.0
14	3029.0
15	2061.0
16	2061.0
17	2061.0
18	3625.0
19	5189.0
20	5189.0
21	5189.0
22	8673.5
23	12158.0
24	12158.0
25	12158.0
26	18766.5
27	25375.0
28	25375.0
29	25375.0
30	31163.0
31	36951.0
32	36951.0
33	36951.0
34	42283.5
35	47616.0
36	47616.0
37	47616.0
38	53179.0
39	58742.0
40	58742.0
41	58742.0
42	67934.0
43	77126.0
44	77126.0
45	77126.0
46	88727.0
47	100328.0
48	100328.0
49	100328.0
50	101966.0
51	103604.0
52	103604.0
53	103604.0
54	93382.0
55	83160.0
56	83160.0
57	83160.0
58	77203.0
59	71246.0
60	71246.0
61	71246.0
62	63493.0
63	55740.0
64	55740.0
65	55740.0
66	45919.5
67	36099.0
68	36099.0
69	36099.0
70	27633.5
71	19168.0
72	19168.0
73	19168.0
74	14879.0
75	10590.0
76	10590.0
77	10590.0
78	8712.5
79	6835.0
80	6835.0
81	6835.0
82	4606.0
83	2377.0
84	2377.0
85	2377.0
86	1859.0
87	1341.0
88	1341.0
89	1341.0
90	957.5
91	574.0
92	574.0
93	574.0
94	376.5
95	179.0
96	179.0
97	179.0
98	310.0
99	441.0
100	441.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037790777225974514
2	0.005994399146189061
3	0.0023456344485087628
4	0.00521252099668614
5	0.008600659644532131
6	0.01159785921762666
7	0.020328831887075946
8	0.03609670790205152
9	0.049128010393766866
10	0.05798929608813331
11	0.055122409539955924
12	0.06463526035890814
13	0.06411400825923952
14	0.06776277295691982
15	0.06137743473597929
16	0.06632932968283113
17	0.06476557338382528
18	0.0764937456263691
19	0.07558155445194903
20	0.07779687587554064
21	0.07558155445194903
22	0.07597249352670048
23	0.08313970989714393
24	0.07753624982570632
25	0.07701499772603772
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	767383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.41445252275112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0039029998454	36.56862351284139
2	15.325748835133579	15.14626976378633
3	5.130174286317191	7.605142611139783
4	2.1242528767587747	4.198751716996558
5	1.043196369279705	2.5774488730839162
6	0.5899240764816089	1.7490465161596955
7	0.3312783531430981	1.1458956917243361
8	0.24836108850050456	0.9818101772965576
9	0.17214187705304093	0.7655666949733276
>10	0.8017678272562871	7.534475022605871
>50	0.10536046301861661	3.699370172028258
>100	0.110654734903324	11.078078972699757
>500	0.010853694093275953	3.5570409085696335
>1k	0.002117793969419698	2.626313265716113
>5k	2.6472424617746224E-4	0.7661661003785064
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5857	0.763243386939768	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4394	0.5725954314859725	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4352	0.567122284439452	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2857	0.3723043121883075	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2294	0.2989380791599501	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2102	0.27391797837585663	No Hit
GTACATGGGGTGGTATCAACGCAAA	1733	0.22583247218142702	No Hit
GTATCAACGCAGAGTACATGGGGTG	1219	0.1588515773740101	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1126	0.14673246605671483	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	919	0.11975766989886405	No Hit
GTACATGGGTGGTATCAACGCAAAA	875	0.11402389680250931	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	851	0.11089638420449761	No Hit
GAATAGGACCGCGGTTCTATTTTGT	842	0.10972356698024324	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	840	0.10946294093040893	No Hit
GTATCTGATCGTCTTCGAACCTCCG	832	0.10842043673107171	No Hit
TATCAACGCAGAGTACATGGGGTGG	818	0.10659605438223156	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	808	0.10529292413306002	No Hit
ATCAGATACCGTCGTAGTTCCGACC	794	0.10346854178421988	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	793	0.10333822875930274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.60626049834307E-4
2	0.0	0.0	0.0	0.0	2.60626049834307E-4
3	0.0	0.0	0.0	0.0	2.60626049834307E-4
4	0.0	0.0	0.0	0.0	2.60626049834307E-4
5	0.0	0.0	0.0	0.0	2.60626049834307E-4
6	0.0	0.0	0.0	0.0	5.21252099668614E-4
7	0.0	0.0	0.0	0.0	5.21252099668614E-4
8	0.0	0.0	0.0	0.0	6.515651245857675E-4
9	0.0	0.0	0.0	1.303130249171535E-4	6.515651245857675E-4
10	0.0	0.0	0.0	1.303130249171535E-4	7.81878149502921E-4
11	0.0	0.0	0.0	1.303130249171535E-4	9.121911744200744E-4
12	0.0	0.0	0.0	1.303130249171535E-4	0.0018243823488401489
13	0.0	0.0	0.0	1.303130249171535E-4	0.0024759474734259166
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	70	1.3169483E-9	17.634203	1
GTTATTC	80	7.697963E-9	15.43697	3
CGTTATT	75	5.8578735E-8	15.194525	2
CACGCGC	45	6.7552185E-4	14.7782345	12
ATCGCTC	100	6.548362E-10	14.252298	16
AAATCGC	100	6.566552E-10	14.2513685	14
AAGCCCG	40	0.005277079	14.249511	5
AATCGCT	95	4.8748916E-9	14.002258	15
CAAATCG	95	4.8839865E-9	14.000432	13
CGAACGA	75	9.636769E-7	13.935581	16
GTCCTAA	135	1.8189894E-12	13.3638115	1
TCCTAAG	65	5.463907E-5	13.149108	2
CGCATCG	160	0.0	12.469134	13
CAAGACG	160	0.0	12.468322	4
GTTTTCG	85	3.928866E-6	12.296101	15
GCAAGAC	170	0.0	12.293695	3
CGCAAGA	170	0.0	12.289689	2
AACGAAC	70	1.0885338E-4	12.21546	14
TAACGAA	70	1.0891968E-4	12.2146635	13
TAAATCC	140	5.2750693E-11	12.213867	4
>>END_MODULE