FastQCFastQC Report
Thu 2 Feb 2017
SRR4063925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767383
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19180.2499403817911004No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA11800.15376936940224112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10600.1381318064121827No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10350.13487398078925386No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9190.11975766989886405No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8810.11480577495201222No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8460.11024481907991185No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8260.10763855858156879No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8160.10633542833239724No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8090.10542323715797718No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8000.1042504199337228No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7690.10021071616129104No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7680.10008040313637387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG803.6137626E-621.9992492
CCGTCGT1850.021.4032829
GTCCTAA1551.8189894E-1221.2895971
TCTAAGC856.1138853E-620.7038273
TGTACGT755.4734766E-520.5312965
ACCGTCG1850.020.214218
TTAACGG550.004477959620.0032335
TGACTCG1001.1234097E-619.7980356
CGTCGTA2050.019.31515710
GTCGTAG1950.019.17758411
ATACCGT1950.019.1775846
TACCGTC1950.019.1775847
TACTAGA708.121488E-418.8565032
CGGACCG1051.7915318E-618.856521
CGGTGAC1051.7926486E-618.8552723
CGCAATA2150.018.42157736
CGTAAAC600.007402798618.33629432
GTATTAG2200.017.9993861
GTGTAGG1601.1004886E-917.874391
GTCCTAT2350.017.7866271
Produced by FastQC (version 0.11.5)