##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767383
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.088584188078183	32.0	32.0	32.0	32.0	32.0
2	31.340044019739818	32.0	32.0	32.0	32.0	32.0
3	31.424748789066214	32.0	32.0	32.0	32.0	32.0
4	31.522665995988966	32.0	32.0	32.0	32.0	32.0
5	31.425757411879076	32.0	32.0	32.0	32.0	32.0
6	35.020816202600265	36.0	36.0	36.0	36.0	36.0
7	35.05634995823468	36.0	36.0	36.0	36.0	36.0
8	35.006586020279315	36.0	36.0	36.0	36.0	36.0
9	35.11591604192431	36.0	36.0	36.0	36.0	36.0
10	34.95487911512244	36.0	36.0	36.0	36.0	36.0
11	35.11809878509167	36.0	36.0	36.0	36.0	36.0
12	35.00789957557048	36.0	36.0	36.0	36.0	36.0
13	35.065993122078545	36.0	36.0	36.0	36.0	36.0
14	34.995618876102284	36.0	36.0	36.0	36.0	36.0
15	34.970847673195784	36.0	36.0	36.0	36.0	36.0
16	34.958480967131145	36.0	36.0	36.0	36.0	36.0
17	34.9320495762872	36.0	36.0	36.0	32.0	36.0
18	34.925162532920325	36.0	36.0	36.0	36.0	36.0
19	34.920523389233274	36.0	36.0	36.0	32.0	36.0
20	34.903991878892285	36.0	36.0	36.0	32.0	36.0
21	34.876959745003475	36.0	36.0	36.0	32.0	36.0
22	34.87509496561691	36.0	36.0	36.0	32.0	36.0
23	34.8259578333114	36.0	36.0	36.0	32.0	36.0
24	34.802143128007785	36.0	36.0	36.0	32.0	36.0
25	34.769907595034034	36.0	36.0	36.0	32.0	36.0
26	34.70534661309932	36.0	36.0	36.0	32.0	36.0
27	34.709747283950776	36.0	36.0	36.0	32.0	36.0
28	34.677238093624695	36.0	36.0	36.0	32.0	36.0
29	34.64060710232049	36.0	36.0	36.0	32.0	36.0
30	34.610749782051464	36.0	36.0	36.0	32.0	36.0
31	34.604858330194965	36.0	36.0	36.0	32.0	36.0
32	34.537134651145514	36.0	36.0	36.0	32.0	36.0
33	34.47909062358692	36.0	36.0	36.0	32.0	36.0
34	34.44552459462876	36.0	36.0	36.0	32.0	36.0
35	34.382191161388775	36.0	36.0	36.0	32.0	36.0
36	34.36330098529678	36.0	36.0	36.0	32.0	36.0
37	34.34722687367325	36.0	36.0	36.0	32.0	36.0
38	34.271339083612745	36.0	36.0	36.0	32.0	36.0
39	34.26207904006213	36.0	36.0	36.0	32.0	36.0
40	34.22441075707958	36.0	36.0	36.0	32.0	36.0
41	34.17783557884394	36.0	36.0	36.0	32.0	36.0
42	34.087731940895225	36.0	36.0	36.0	32.0	36.0
43	34.062344357380866	36.0	36.0	36.0	32.0	36.0
44	33.9625662804623	36.0	36.0	36.0	32.0	36.0
45	33.90496922657917	36.0	36.0	36.0	32.0	36.0
46	33.8370435623411	36.0	36.0	36.0	32.0	36.0
47	33.769555749866754	36.0	36.0	36.0	27.0	36.0
48	33.69141484760544	36.0	36.0	36.0	27.0	36.0
49	33.630776287720735	36.0	36.0	36.0	27.0	36.0
50	32.9840405638384	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	6.0
21	28.0
22	96.0
23	280.0
24	756.0
25	1753.0
26	3577.0
27	6434.0
28	10795.0
29	16187.0
30	23417.0
31	32322.0
32	46009.0
33	72952.0
34	144213.0
35	408555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.356867130096624	18.177375339166606	11.936579428193118	25.529178102543653
2	15.732231749589513	21.05202116291798	37.135708514686335	26.08003857280617
3	18.54869816779171	25.64296697855039	28.51181943756679	27.296515416091115
4	11.976418555010993	15.97533435064368	35.39718758429623	36.65105951004909
5	13.914303548553983	37.574457604612036	32.50163217063709	16.009606676196892
6	33.45500226093098	35.67175712779668	17.086904453187003	13.786336158085335
7	29.642081779348484	30.882663393199216	20.891290126690485	18.58396470076181
8	28.760996790390198	32.033287159084836	19.493134458282242	19.712581592242724
9	28.20515159491982	13.555841089221163	18.635763004740845	39.60324431111818
10	16.481795199249394	26.911829862649533	30.784487476869348	25.821887461231725
11	37.236164991927104	21.239459305197013	22.42582908404278	19.098546618833097
12	25.34500418305355	24.166842589479558	28.58354769853867	21.904605528928226
13	29.99791498345018	19.355338945502883	25.770804555761163	24.875941515285778
14	24.034183757242154	19.810342176386726	25.785593094443183	30.36988097192793
15	25.14869394329561	27.150653940584974	23.4296564270709	24.270995689048515
16	25.03743280664603	25.697866101971005	24.861638703371884	24.403062388011076
17	23.305832941308317	25.781128849609647	25.796636099574787	25.116402109507245
18	24.200396153144467	24.766869087023377	27.62686022570304	23.405874534129115
19	25.381491877578572	24.634145705789294	26.468564548034745	23.51579786859739
20	26.002926845795884	23.588604848451677	26.104050280239623	24.304418025512817
21	27.333678228472614	23.5427420206077	25.21192155677152	23.911658194148163
22	25.85462539566292	23.99010663514829	25.80289112477081	24.35237684441798
23	24.244078840201986	23.969115491122334	26.610327414888417	25.17647825378726
24	24.54758339751283	25.103892600657563	26.196312382799114	24.152211619030492
25	24.684277536042075	24.472775086757707	26.35101587636685	24.49193150083337
26	24.042020267311663	25.67714089117788	26.85523050939344	23.42560833211702
27	24.58919400051868	24.94536270432785	26.204920158523095	24.26052313663038
28	23.600090180138736	25.169902248378516	26.838495451246036	24.391512120236712
29	23.54042730643175	24.92881484039538	26.770050758113804	24.760707095059065
30	23.63820468077842	25.5748673032289	26.787610884718987	23.999317131273692
31	24.265341203056316	25.458831870032284	25.533115521503813	24.74271140540759
32	23.883792726424474	25.603731268399287	25.478886509859215	25.033589495317017
33	23.730432606848524	25.075845639787946	26.189290908427466	25.004430844936067
34	24.522063902043627	25.26017707418402	26.245500819691358	23.972258204080994
35	25.036456113876	25.17902265035622	26.29466444085639	23.48985679491139
36	23.727991867905594	26.2184457795212	25.710189879191482	24.343372473381727
37	24.469088824020016	26.133757297748122	25.58825062552127	23.808903252710593
38	23.847261590590687	25.829602841038675	25.768481412699963	24.554654155670676
39	24.36149722027466	25.626773980832535	25.557052787414218	24.454676011478586
40	24.79419725526838	26.157831841858005	25.81496844379634	23.23300245907728
41	23.42255229923778	26.281824899037353	26.51965236791453	23.775970433810333
42	25.11962907168883	26.74387389688443	25.350156117241703	22.786340914185036
43	23.85538212136455	25.826259425000064	25.81479174431242	24.503566709322964
44	23.58584614662867	26.583108212889005	25.253627012889527	24.577418627592795
45	23.87541358743033	26.588051933158795	25.377685630548978	24.158848848861894
46	23.624595047525716	26.262552549343603	25.24218959724984	24.870662805880837
47	23.96430889202228	26.104889217865885	25.638095188450823	24.292706701661015
48	24.745852386531123	26.82490431591581	24.29066068605847	24.1385826114946
49	23.848679923896896	27.129453465036878	24.645938127133885	24.37592848393234
50	23.80732085867425	27.166200354718523	24.48039991190679	24.546078874700438
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	76.0
2	46.0
3	156.0
4	266.0
5	313.5
6	361.0
7	364.5
8	368.0
9	474.5
10	581.0
11	899.0
12	1217.0
13	1968.0
14	2719.0
15	3768.0
16	4817.0
17	5242.5
18	5668.0
19	5786.5
20	5905.0
21	6245.5
22	6586.0
23	7184.5
24	7783.0
25	7086.0
26	6389.0
27	7114.5
28	7840.0
29	9097.0
30	10354.0
31	11848.5
32	13343.0
33	15890.0
34	18437.0
35	20802.0
36	23167.0
37	26129.0
38	29091.0
39	30249.0
40	31407.0
41	33915.5
42	36424.0
43	37287.5
44	38151.0
45	42385.0
46	46619.0
47	49890.5
48	53162.0
49	55320.5
50	57479.0
51	54916.5
52	52354.0
53	51846.0
54	51338.0
55	51865.0
56	52392.0
57	50903.0
58	49414.0
59	45445.0
60	41476.0
61	36524.0
62	31572.0
63	27764.5
64	23957.0
65	20678.5
66	17400.0
67	14840.5
68	12281.0
69	11201.0
70	10121.0
71	7897.0
72	5673.0
73	4941.0
74	4209.0
75	3308.5
76	2408.0
77	2080.5
78	1753.0
79	1442.0
80	1131.0
81	918.0
82	705.0
83	574.5
84	444.0
85	337.5
86	231.0
87	163.5
88	96.0
89	67.5
90	39.0
91	36.5
92	34.0
93	27.0
94	20.0
95	23.5
96	27.0
97	19.0
98	11.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007949094519946364
2	3.909390747514604E-4
3	3.909390747514604E-4
4	0.0
5	0.0
6	0.0
7	1.303130249171535E-4
8	0.0
9	0.0011728172242543814
10	3.909390747514604E-4
11	0.0
12	1.303130249171535E-4
13	3.909390747514604E-4
14	1.303130249171535E-4
15	0.004560955872100372
16	0.001042504199337228
17	0.0
18	3.909390747514604E-4
19	1.303130249171535E-4
20	5.21252099668614E-4
21	0.0
22	0.0
23	0.001042504199337228
24	5.21252099668614E-4
25	0.002085008398674456
26	0.0050822079717689865
27	0.006515651245857675
28	0.0039093907475146045
29	0.0023456344485087628
30	0.004170016797348912
31	0.007036903345526288
32	0.0039093907475146045
33	0.004560955872100372
34	0.0027365735232602233
35	0.002866886548177377
36	0.006906590320609135
37	0.0029971995730945305
38	0.007558155445194903
39	0.005342834021603293
40	0.002866886548177377
41	0.00260626049834307
42	0.0014334432740886886
43	0.0011728172242543814
44	0.002085008398674456
45	0.0015637562990058417
46	0.0011728172242543814
47	0.0027365735232602233
48	0.001824382348840149
49	3.909390747514604E-4
50	0.001824382348840149
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	767383.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.24555665237308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.32449591236144	42.16589262890182
2	14.24133746371373	15.735412313143202
3	4.574120570570988	7.58099511348794
4	1.9480471769721606	4.304838027076439
5	0.925754681828419	2.5571916360575773
6	0.4870681506073753	1.6145010664767805
7	0.3085097414648248	1.1930654679932768
8	0.19047758727501776	0.8418432271047468
9	0.14152855652003432	0.7036941498440528
>10	0.6784686567488797	7.0878515924882075
>50	0.08285516683126018	3.2309168629948473
>100	0.08902713576860638	9.609807295395962
>500	0.00735957655591358	2.6928970025278844
>1k	9.49622781408204E-4	0.6810936165072717
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1918	0.2499403817911004	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1180	0.15376936940224112	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1060	0.1381318064121827	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1035	0.13487398078925386	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	919	0.11975766989886405	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	881	0.11480577495201222	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	846	0.11024481907991185	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	826	0.10763855858156879	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	816	0.10633542833239724	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	809	0.10542323715797718	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	800	0.1042504199337228	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	769	0.10021071616129104	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	768	0.10008040313637387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.60626049834307E-4
2	0.0	0.0	0.0	0.0	2.60626049834307E-4
3	0.0	0.0	0.0	0.0	2.60626049834307E-4
4	0.0	0.0	0.0	0.0	2.60626049834307E-4
5	0.0	0.0	0.0	0.0	2.60626049834307E-4
6	0.0	0.0	0.0	0.0	3.909390747514605E-4
7	0.0	0.0	0.0	0.0	3.909390747514605E-4
8	0.0	0.0	0.0	0.0	3.909390747514605E-4
9	0.0	0.0	0.0	0.0	3.909390747514605E-4
10	0.0	0.0	0.0	0.0	3.909390747514605E-4
11	0.0	0.0	0.0	0.0	3.909390747514605E-4
12	0.0	0.0	0.0	0.0	0.001303130249171535
13	0.0	0.0	0.0	0.0	0.0014334432740886883
14	0.0	0.0	0.0	0.0	0.0014334432740886883
15	0.0	0.0	0.0	1.303130249171535E-4	0.0023456344485087628
16	0.0	0.0	0.0	5.21252099668614E-4	0.0023456344485087628
17	0.0	0.0	0.0	6.515651245857675E-4	0.0024759474734259166
18	0.0	0.0	0.0	6.515651245857675E-4	0.0024759474734259166
19	0.0	0.0	0.0	7.81878149502921E-4	0.0024759474734259166
20	0.0	0.0	0.0	9.121911744200744E-4	0.00260626049834307
21	0.0	0.0	0.0	9.121911744200744E-4	0.00260626049834307
22	0.0	0.0	0.0	0.0022153214235916094	0.0027365735232602233
23	0.0	0.0	0.0	0.00521252099668614	0.0027365735232602233
24	0.0	0.0	0.0	0.008600659644532131	0.0027365735232602233
25	0.0	0.0	0.0	0.010164415943537973	0.0027365735232602233
26	0.0	0.0	0.0	0.012770676441881042	0.0029971995730945305
27	0.0	0.0	0.0	0.015767876014975573	0.003257825622928837
28	0.0	0.0	0.0	0.023195718435253324	0.003257825622928837
29	0.0	0.0	0.0	0.036357333951885826	0.003388138647845991
30	0.0	0.0	0.0	0.06541713850841105	0.003388138647845991
31	0.0	0.0	0.0	0.12210330434737282	0.0036487646976802977
32	0.0	0.0	0.0	0.18048353951025758	0.0036487646976802977
33	0.0	0.0	0.0	0.2331300015767876	0.0037790777225974515
34	0.0	0.0	0.0	0.29294368001376103	0.004170016797348912
35	0.0	0.0	0.0	0.36213989624476955	0.004170016797348912
36	0.0	0.0	0.0	0.4553137090605343	0.004170016797348912
37	0.0	0.0	0.0	0.5831507865042619	0.004170016797348912
38	0.0	0.0	0.0	0.7517758407470585	0.004170016797348912
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGG	80	3.6137626E-6	21.999249	2
CCGTCGT	185	0.0	21.403282	9
GTCCTAA	155	1.8189894E-12	21.289597	1
TCTAAGC	85	6.1138853E-6	20.703827	3
TGTACGT	75	5.4734766E-5	20.531296	5
ACCGTCG	185	0.0	20.21421	8
TTAACGG	55	0.0044779596	20.00323	35
TGACTCG	100	1.1234097E-6	19.798035	6
CGTCGTA	205	0.0	19.315157	10
GTCGTAG	195	0.0	19.177584	11
ATACCGT	195	0.0	19.177584	6
TACCGTC	195	0.0	19.177584	7
TACTAGA	70	8.121488E-4	18.856503	2
CGGACCG	105	1.7915318E-6	18.8565	21
CGGTGAC	105	1.7926486E-6	18.855272	3
CGCAATA	215	0.0	18.421577	36
CGTAAAC	60	0.0074027986	18.336294	32
GTATTAG	220	0.0	17.999386	1
GTGTAGG	160	1.1004886E-9	17.87439	1
GTCCTAT	235	0.0	17.786627	1
>>END_MODULE