FastQCFastQC Report
Thu 2 Feb 2017
SRR4063923_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063923_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4896322
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81680.16681909400566383No Hit
TATCAACGCAGAGTACTTTTTTTTT50920.10399642833947603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.013.7200751
GTATCAA53100.013.1476591
ACGGTAT6050.011.4639449
CAAGACG9450.011.3573144
CGAACGA4750.011.00165216
TAACGAA4900.010.85721913
ATAACGA5250.010.85721912
TACGGGT1053.4796467E-610.8547784
CGGTCCA8600.010.82609910
GTCCTAT8850.010.7327831
CGCATCG5100.010.61772213
CGAGCCG8700.010.59354415
AAGACGG10050.010.585845
AGGCCCG9000.010.55606110
TCGGCGT5400.010.5556313
TAAGGCG1355.7070793E-810.5543355
ACCGTCG6850.010.5410818
GATAACG5250.010.49520411
GTATAAT3550.010.4349871
AATTCCG5750.010.4074935
Produced by FastQC (version 0.11.5)