##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063923_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4896322
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312939590165843	32.0	32.0	32.0	32.0	32.0
2	30.918587257945862	32.0	32.0	32.0	32.0	32.0
3	30.935839391281863	32.0	32.0	32.0	32.0	32.0
4	30.970611205717272	32.0	32.0	32.0	32.0	32.0
5	30.877948795034314	32.0	32.0	32.0	32.0	32.0
6	34.549539634035504	36.0	36.0	36.0	32.0	36.0
7	34.503302887350955	36.0	36.0	36.0	32.0	36.0
8	34.46280943124247	36.0	36.0	36.0	32.0	36.0
9	34.60807561267416	36.0	36.0	36.0	32.0	36.0
10	34.30431822090132	36.0	36.0	36.0	32.0	36.0
11	34.57109091273001	36.0	36.0	36.0	32.0	36.0
12	34.38990062336587	36.0	36.0	36.0	32.0	36.0
13	34.47076662850196	36.0	36.0	36.0	32.0	36.0
14	34.36989826241003	36.0	36.0	36.0	32.0	36.0
15	34.30391567384661	36.0	36.0	36.0	32.0	36.0
16	34.30825832124603	36.0	36.0	36.0	32.0	36.0
17	34.23221369019439	36.0	36.0	36.0	32.0	36.0
18	34.22511039919352	36.0	36.0	36.0	32.0	36.0
19	34.250736573289096	36.0	36.0	36.0	32.0	36.0
20	34.233896790284625	36.0	36.0	36.0	32.0	36.0
21	34.216398145383415	36.0	36.0	36.0	32.0	36.0
22	34.203387971624416	36.0	36.0	36.0	32.0	36.0
23	34.14121436457815	36.0	36.0	36.0	32.0	36.0
24	34.1238125270356	36.0	36.0	36.0	32.0	36.0
25	33.72963093522036	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	15.0
4	44.0
5	66.0
6	205.0
7	76.0
8	265.0
9	342.0
10	449.0
11	117.0
12	326.0
13	241.0
14	380.0
15	676.0
16	1283.0
17	1604.0
18	2122.0
19	2827.0
20	4327.0
21	6432.0
22	10231.0
23	15802.0
24	23638.0
25	35290.0
26	50083.0
27	66972.0
28	91472.0
29	124456.0
30	163802.0
31	223362.0
32	318407.0
33	454700.0
34	998580.0
35	2297729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.93016294876085	16.584736665534766	10.982359101851046	26.502741283853332
2	17.348561069233952	19.003787939133172	36.53996902819529	27.107681963437585
3	18.964665073060264	22.677254672572705	28.39616260013823	29.961917654228802
4	12.879683145150386	14.986125754219358	35.05782739890877	37.07636370172149
5	15.049467497004091	35.83615154168609	33.55997468055028	15.554406280759526
6	35.458574796264216	34.76972244299514	16.23029205958969	13.541410701150955
7	31.18045698904962	29.97702634350744	20.26656060297779	18.575956064465153
8	28.65706356159368	32.553721216836436	18.820977221909853	19.96823799966003
9	27.387998415149507	14.040659711388018	18.02183031433209	40.549511559130394
10	15.774771768324694	26.327471199372702	31.507343692492494	26.39041333981011
11	37.69255903951853	20.752451810302745	22.10098068325163	19.4540084669271
12	24.917062679217267	23.275299178259907	28.289346358064016	23.518291784458814
13	29.561894843072285	19.04505759088045	24.963432820721803	26.42961474532546
14	23.825084724701433	19.121172571065127	24.71155178538071	32.34219091885272
15	25.578312369094846	26.76899364911401	21.714442896079216	25.938251085711926
16	26.312318336752515	25.414849267788025	23.017824304279067	25.255008091180386
17	24.601464084558852	25.654219359268858	24.545406240548033	25.198910315624257
18	25.293321696260122	24.737910355019306	25.245185910420425	24.723582038300144
19	25.86085248882392	24.639393597504146	24.84871004980591	24.651043863866022
20	26.101987104749792	24.145542504778895	24.28989049012089	25.462579900350423
21	26.97138052230588	23.995403682123168	24.13743415582515	24.895781639745802
22	26.327025963607003	23.775285262420986	24.464330061268186	25.433358712703825
23	25.004548005888495	23.80757417746503	24.991323017979035	26.196554798667442
24	25.173585384632837	24.49870200584603	24.61673863664932	25.71097397287181
25	25.269896554472933	24.107508947496438	24.801785615736964	25.820808882293665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	201.0
2	557.5
3	914.0
4	914.0
5	914.0
6	2542.0
7	4170.0
8	4170.0
9	4170.0
10	5189.5
11	6209.0
12	6209.0
13	6209.0
14	8679.5
15	11150.0
16	11150.0
17	11150.0
18	19066.0
19	26982.0
20	26982.0
21	26982.0
22	42011.0
23	57040.0
24	57040.0
25	57040.0
26	84987.0
27	112934.0
28	112934.0
29	112934.0
30	144627.5
31	176321.0
32	176321.0
33	176321.0
34	225979.0
35	275637.0
36	275637.0
37	275637.0
38	325691.0
39	375745.0
40	375745.0
41	375745.0
42	437380.0
43	499015.0
44	499015.0
45	499015.0
46	563494.0
47	627973.0
48	627973.0
49	627973.0
50	651954.5
51	675936.0
52	675936.0
53	675936.0
54	645291.0
55	614646.0
56	614646.0
57	614646.0
58	569657.0
59	524668.0
60	524668.0
61	524668.0
62	460152.0
63	395636.0
64	395636.0
65	395636.0
66	323737.5
67	251839.0
68	251839.0
69	251839.0
70	190072.0
71	128305.0
72	128305.0
73	128305.0
74	97242.5
75	66180.0
76	66180.0
77	66180.0
78	51735.5
79	37291.0
80	37291.0
81	37291.0
82	25569.5
83	13848.0
84	13848.0
85	13848.0
86	10482.5
87	7117.0
88	7117.0
89	7117.0
90	5045.5
91	2974.0
92	2974.0
93	2974.0
94	2060.0
95	1146.0
96	1146.0
97	1146.0
98	1795.5
99	2445.0
100	2445.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038804637440103
2	0.005228414307719141
3	0.0018381144050575104
4	0.004084698677905578
5	0.009823700320362918
6	0.013132306249466437
7	0.02348701739795708
8	0.03663974714081304
9	0.05075238107297682
10	0.060024647071822486
11	0.055000467697998615
12	0.0647220505514139
13	0.0668460938639248
14	0.06976665341862728
15	0.06104582174129888
16	0.06821446792102316
17	0.06457908609768721
18	0.07989670613983313
19	0.07617963034293905
20	0.08124465670354197
21	0.0757711604751485
22	0.0795903537389902
23	0.08308277110859948
24	0.07705784055868875
25	0.07948823627204256
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4896322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.9209413313136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.52834327994167	26.671876283307284
2	15.408859308993225	11.994546182955814
3	6.04528096125387	7.058640768728069
4	3.0152656323662375	4.694279071026101
5	1.6474232045113213	3.205963094531489
6	1.092549034510547	2.551382212426099
7	0.7350931871496751	2.0027363167070584
8	0.5054944945849109	1.5739457253633071
9	0.3943759541265903	1.381453503573766
>10	2.333865134615177	16.68586503705769
>50	0.17310558464058826	4.644197941100264
>100	0.09818427184380973	7.39231803196785
>500	0.012873877349953166	3.474895273976813
>1k	0.00918055054312335	6.395601374981295
>5k	1.0552356946117763E-4	0.27229918229739397
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8168	0.16681909400566383	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5092	0.10399642833947603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	2.0423493389527894E-5
10	0.0	0.0	0.0	0.0	2.0423493389527894E-5
11	0.0	0.0	0.0	0.0	2.0423493389527894E-5
12	0.0	0.0	0.0	2.0423493389527894E-5	8.169397355811157E-5
13	0.0	0.0	0.0	2.0423493389527894E-5	1.0211746694763947E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1800	0.0	13.720075	1
GTATCAA	5310	0.0	13.147659	1
ACGGTAT	605	0.0	11.463944	9
CAAGACG	945	0.0	11.357314	4
CGAACGA	475	0.0	11.001652	16
TAACGAA	490	0.0	10.857219	13
ATAACGA	525	0.0	10.857219	12
TACGGGT	105	3.4796467E-6	10.854778	4
CGGTCCA	860	0.0	10.826099	10
GTCCTAT	885	0.0	10.732783	1
CGCATCG	510	0.0	10.617722	13
CGAGCCG	870	0.0	10.593544	15
AAGACGG	1005	0.0	10.58584	5
AGGCCCG	900	0.0	10.556061	10
TCGGCGT	540	0.0	10.55563	13
TAAGGCG	135	5.7070793E-8	10.554335	5
ACCGTCG	685	0.0	10.541081	8
GATAACG	525	0.0	10.495204	11
GTATAAT	355	0.0	10.434987	1
AATTCCG	575	0.0	10.407493	5
>>END_MODULE