FastQCFastQC Report
Thu 2 Feb 2017
SRR4063923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4896322
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58150.027.317531
GGTATCA24450.024.3862131
TCAACGC83550.018.9312234
CAACGCA85300.018.4656545
TATCAAC87200.018.31542
AACGCAG86000.018.3153516
ATCAACG88300.017.962673
CGGATCG2900.016.69068526
ATACCGT10600.016.187866
ATACGAA10900.015.94430940
CGCGAAA850.002982277715.52894314
ACCGTCG10200.015.5287858
TTAACGG4850.015.42363335
TACCGTC11050.015.3296987
ACGCAGA103700.015.2537
CGTCGTA10550.015.22244510
CGCAATA11600.015.1733536
TAACGGC4950.015.11204436
CGCAGAG105550.014.9856588
CTAGCGG11750.014.97934229
Produced by FastQC (version 0.11.5)