##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063923_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4896322
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3413078224839	32.0	32.0	32.0	32.0	32.0
2	31.44185349738028	32.0	32.0	32.0	32.0	32.0
3	31.506049847211845	32.0	32.0	32.0	32.0	32.0
4	31.585797257615003	32.0	32.0	32.0	32.0	32.0
5	31.543390528645787	32.0	32.0	32.0	32.0	32.0
6	35.145521475099066	36.0	36.0	36.0	36.0	36.0
7	35.159938623317665	36.0	36.0	36.0	36.0	36.0
8	35.10883271157412	36.0	36.0	36.0	36.0	36.0
9	35.20244134270581	36.0	36.0	36.0	36.0	36.0
10	35.070970822588876	36.0	36.0	36.0	36.0	36.0
11	35.20545033598689	36.0	36.0	36.0	36.0	36.0
12	35.11119816057849	36.0	36.0	36.0	36.0	36.0
13	35.15518750605046	36.0	36.0	36.0	36.0	36.0
14	35.09693582243978	36.0	36.0	36.0	36.0	36.0
15	35.07173874594032	36.0	36.0	36.0	36.0	36.0
16	35.08663502931384	36.0	36.0	36.0	36.0	36.0
17	35.051571567392834	36.0	36.0	36.0	36.0	36.0
18	35.04657863596389	36.0	36.0	36.0	36.0	36.0
19	35.048324027708965	36.0	36.0	36.0	36.0	36.0
20	35.0401977239242	36.0	36.0	36.0	36.0	36.0
21	35.036915055831706	36.0	36.0	36.0	36.0	36.0
22	35.02155168716437	36.0	36.0	36.0	36.0	36.0
23	34.967604459020464	36.0	36.0	36.0	36.0	36.0
24	34.942952485559566	36.0	36.0	36.0	36.0	36.0
25	34.91803949985316	36.0	36.0	36.0	32.0	36.0
26	34.858054270123574	36.0	36.0	36.0	32.0	36.0
27	34.853479611839255	36.0	36.0	36.0	32.0	36.0
28	34.835338852305874	36.0	36.0	36.0	32.0	36.0
29	34.809898123530274	36.0	36.0	36.0	32.0	36.0
30	34.79319701604592	36.0	36.0	36.0	32.0	36.0
31	34.78815220894378	36.0	36.0	36.0	32.0	36.0
32	34.75615941925388	36.0	36.0	36.0	32.0	36.0
33	34.73306657527834	36.0	36.0	36.0	32.0	36.0
34	34.73048116525016	36.0	36.0	36.0	32.0	36.0
35	34.69560519099847	36.0	36.0	36.0	32.0	36.0
36	34.67524705278779	36.0	36.0	36.0	32.0	36.0
37	34.67076695527786	36.0	36.0	36.0	32.0	36.0
38	34.63306967964934	36.0	36.0	36.0	32.0	36.0
39	34.638400211424006	36.0	36.0	36.0	32.0	36.0
40	34.62150896121619	36.0	36.0	36.0	32.0	36.0
41	34.60979322846823	36.0	36.0	36.0	32.0	36.0
42	34.54510753173505	36.0	36.0	36.0	32.0	36.0
43	34.53715523611397	36.0	36.0	36.0	32.0	36.0
44	34.48945698424246	36.0	36.0	36.0	32.0	36.0
45	34.46623935272231	36.0	36.0	36.0	32.0	36.0
46	34.45017423282211	36.0	36.0	36.0	32.0	36.0
47	34.43282651753704	36.0	36.0	36.0	32.0	36.0
48	34.38763688335857	36.0	36.0	36.0	32.0	36.0
49	34.377566671473	36.0	36.0	36.0	32.0	36.0
50	33.78929633304345	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	6.0
18	11.0
19	14.0
20	38.0
21	146.0
22	500.0
23	1556.0
24	3893.0
25	8760.0
26	17628.0
27	31457.0
28	52130.0
29	80223.0
30	114899.0
31	162893.0
32	234400.0
33	379985.0
34	807374.0
35	3000407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9833345180058	17.407066530388484	11.528619644157073	27.08097930744865
2	16.581166661492688	19.682674413475002	36.153676166636984	27.582482758395326
3	18.261273241240797	23.195257160141306	28.619549386915487	29.92392021170241
4	12.529997005915869	15.505352793382462	35.16941900471415	36.79523119598752
5	14.608536775154901	36.270102333955975	33.361776451793816	15.759584439095303
6	35.511011852791334	35.08791815886029	16.0507647077445	13.350305280603884
7	31.117816381874203	30.026812005929358	20.274332896133703	18.581038716062736
8	28.703667773483854	32.65847303343203	18.683391329246728	19.954467863837387
9	27.520123489972363	13.823855230146718	17.892055745314593	40.763965534566324
10	15.695519242198653	26.581397676190242	31.49877355619686	26.224309525414245
11	37.99432267614807	20.653547837243515	21.998598539597385	19.35353094701103
12	24.880021992053614	23.288232739975417	28.296469548317365	23.5352757196536
13	29.68819918881588	19.01242142279301	24.968042188600002	26.331337199791104
14	23.8428948796993	19.012710377643263	24.677359336022974	32.46703540663446
15	25.616579815617342	26.868039644436966	21.632406218635893	25.882974321309803
16	26.389427563257733	25.465460607943296	22.929882059416958	25.215229769382013
17	24.705401319602753	25.6361816073371	24.493548422673182	25.164868650386964
18	25.421082406955442	24.705441444679767	25.22087041057449	24.652605737790296
19	25.884087256534038	24.63135076315462	24.845123512122676	24.639438468188665
20	26.262034388957662	24.048783147862633	24.249731581990495	25.439450881189213
21	27.165513215838338	23.87163262546867	24.105685859712658	24.857168298980337
22	26.36444476395917	23.806152955059666	24.48272312600867	25.346679154972495
23	25.089767000994833	23.71435743043657	24.993408270389377	26.20246729817921
24	25.263888415174502	24.464732809086385	24.636985572554586	25.63439320318453
25	25.376474403907018	23.99107800799638	24.856664820342893	25.775782767753707
26	25.23959586254817	24.77669275500673	24.944889326247633	25.038822056197468
27	25.77995605812321	24.310515515379556	24.37528250027114	25.534245926226095
28	25.300805681908933	24.20503580297874	25.055651425491426	25.438507089620906
29	25.107292587237712	24.47408608038371	24.927744853090903	25.490876479287678
30	25.018254216749597	24.600577313220302	25.13986048978693	25.241307980243167
31	25.599091984376475	24.38169826454554	24.293931863288666	25.725277887789318
32	25.441632531016197	24.333966755934046	24.16561005458981	26.05879065845995
33	25.07322684421253	24.199304464973764	24.628422656705244	26.099046034108465
34	25.644237462052015	24.149683344716365	24.85809333651405	25.34798585671757
35	26.00072219803095	24.042822095011523	24.920550046934704	25.035905660022827
36	25.10061397811248	24.567723460663323	24.57511734212429	25.75654521909991
37	25.974958594857856	24.230377005844	24.53326922829475	25.261395171003393
38	25.263290550789	24.167868687450493	24.820963242469958	25.747877519290547
39	25.82417010085768	24.15666160890683	24.288929960068277	25.73023833016721
40	25.898849027185538	24.333269474364435	24.756556403059836	25.011325095390184
41	25.030360490228475	24.67181464027159	25.043227701021575	25.254597168478355
42	26.14214799738983	24.55768565030069	24.588403130160216	24.711763222149262
43	25.5172775786575	23.814201822248375	24.796564070650675	25.87195652844345
44	25.085442941026297	24.26638754148583	24.709706407965278	25.93846310952259
45	25.210400688036337	24.481047037397783	24.805499225632335	25.503053048933545
46	25.110517689468015	24.244246901981608	24.912571291440944	25.732664117109433
47	25.201910531944183	24.22971315878264	25.135777990730823	25.432598318542354
48	25.864276150087534	24.755077165398074	24.276852668049496	25.103794016464892
49	25.110425196490166	24.62381295594368	24.57395899475951	25.69180285280664
50	25.080040749503297	24.83989807722472	24.62779723936003	25.45226393391195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	84.0
2	121.0
3	306.5
4	492.0
5	595.0
6	698.0
7	637.5
8	577.0
9	767.5
10	958.0
11	1425.0
12	1892.0
13	3453.0
14	5014.0
15	6523.5
16	8033.0
17	8057.5
18	8082.0
19	8160.5
20	8239.0
21	9633.5
22	11028.0
23	13134.0
24	15240.0
25	19503.5
26	23767.0
27	29547.0
28	35327.0
29	41265.5
30	47204.0
31	53980.0
32	60756.0
33	70786.0
34	80816.0
35	95064.0
36	109312.0
37	131644.5
38	153977.0
39	174566.0
40	195155.0
41	216651.0
42	238147.0
43	249216.5
44	260286.0
45	287545.0
46	314804.0
47	337737.0
48	360670.0
49	376961.0
50	393252.0
51	390208.0
52	387164.0
53	383463.0
54	379762.0
55	380280.5
56	380799.0
57	369145.5
58	357492.0
59	329543.5
60	301595.0
61	264363.5
62	227132.0
63	197246.0
64	167360.0
65	143021.5
66	118683.0
67	101262.0
68	83841.0
69	74504.0
70	65167.0
71	49784.5
72	34402.0
73	29249.5
74	24097.0
75	18437.5
76	12778.0
77	10864.0
78	8950.0
79	7432.5
80	5915.0
81	4679.5
82	3444.0
83	2796.0
84	2148.0
85	1471.5
86	795.0
87	561.0
88	327.0
89	260.5
90	194.0
91	168.0
92	142.0
93	109.5
94	77.0
95	77.0
96	77.0
97	57.5
98	38.0
99	43.5
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009272265998845664
2	5.310108281277253E-4
3	3.063524008429184E-4
4	0.0
5	0.0
6	1.4296445372669525E-4
7	1.6338794711622315E-4
8	0.0
9	0.0011028686430345064
10	0.0010620216562554506
11	2.0423493389527894E-5
12	1.6338794711622315E-4
13	4.697403479591416E-4
14	1.6338794711622315E-4
15	0.004084698677905578
16	8.782102157496995E-4
17	0.0
18	2.4508192067433475E-4
19	2.0423493389527894E-5
20	8.373632289706437E-4
21	0.0
22	1.6338794711622315E-4
23	7.148222686334763E-4
24	5.922813082963089E-4
25	0.0021648902992899566
26	0.005412225748224892
27	0.00631085945736412
28	0.004513592039085665
29	0.002675477634028154
30	0.0039417342241788835
31	0.007577116047514849
32	0.003471993876219742
33	0.004799520946539055
34	0.0029818300348710726
35	0.003226911955545407
36	0.007801774474799655
37	0.0039008872373998277
38	0.008169397355811157
39	0.004391051078748497
40	0.0023691252331852356
41	0.0031860649687663516
42	0.001776843924888927
43	0.0012866800835402573
44	0.0014704915240460084
45	0.0014092210438774247
46	9.59904189307811E-4
47	0.001919808378615622
48	0.001756420431499399
49	4.2889336118008575E-4
50	0.001347950563708841
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4896322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.17205094338471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2769214012456	29.63721909477573
2	14.929913864144718	12.592501761181135
3	5.643714797264549	7.140210838205241
4	2.7655864896633244	4.665218173216729
5	1.597418681829825	3.3683211014020915
6	0.981321913967701	2.483061464862341
7	0.6887036760761166	2.0330832558664818
8	0.48364563282828493	1.6317062612943978
9	0.3650630623019401	1.38559122548509
>10	2.0218761951224176	15.5905311592462
>50	0.14547201060935389	4.214437041474213
>100	0.08249344943602056	6.703076820845653
>500	0.01054553747275389	3.0544709516695985
>1k	0.007323288037342327	5.500570850475014
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.169397355811157E-5
2	0.0	0.0	0.0	0.0	1.0211746694763947E-4
3	0.0	0.0	0.0	0.0	1.0211746694763947E-4
4	0.0	0.0	0.0	0.0	1.0211746694763947E-4
5	0.0	0.0	0.0	0.0	1.2254096033716738E-4
6	0.0	0.0	0.0	0.0	1.2254096033716738E-4
7	0.0	0.0	0.0	0.0	1.2254096033716738E-4
8	0.0	0.0	0.0	0.0	1.2254096033716738E-4
9	0.0	0.0	0.0	2.0423493389527894E-5	1.2254096033716738E-4
10	0.0	0.0	0.0	2.0423493389527894E-5	1.2254096033716738E-4
11	0.0	0.0	0.0	2.0423493389527894E-5	1.2254096033716738E-4
12	0.0	0.0	0.0	4.084698677905579E-5	2.6550541406386265E-4
13	0.0	0.0	0.0	4.084698677905579E-5	3.063524008429184E-4
14	0.0	0.0	0.0	4.084698677905579E-5	3.063524008429184E-4
15	0.0	0.0	0.0	8.169397355811157E-5	4.288933611800858E-4
16	0.0	0.0	0.0	1.0211746694763947E-4	4.288933611800858E-4
17	0.0	0.0	0.0	1.0211746694763947E-4	4.288933611800858E-4
18	0.0	0.0	0.0	1.2254096033716738E-4	4.493168545696137E-4
19	0.0	0.0	0.0	1.2254096033716738E-4	4.493168545696137E-4
20	0.0	0.0	0.0	1.6338794711622315E-4	4.901638413486695E-4
21	0.0	0.0	0.0	1.6338794711622315E-4	5.105873347381974E-4
22	0.0	0.0	0.0	2.8592890745339055E-4	5.514343215172532E-4
23	0.0	0.0	0.0	4.901638413486695E-4	5.718578149067811E-4
24	0.0	0.0	0.0	6.739752818544205E-4	6.127048016858368E-4
25	0.0	0.0	0.0	7.7609274880206E-4	6.127048016858368E-4
26	0.0	0.0	0.0	9.80327682697339E-4	6.127048016858368E-4
27	0.0	0.0	0.0	0.001347950563708841	6.331282950753647E-4
28	0.0	0.0	0.0	0.0031860649687663516	6.535517884648926E-4
29	0.0	0.0	0.0	0.007005258232608068	6.535517884648926E-4
30	0.0	0.0	0.0	0.013908398998268496	6.535517884648926E-4
31	0.0	0.0	0.0	0.0358023839118424	6.739752818544205E-4
32	0.0	0.0	0.0	0.059309824803189005	6.739752818544205E-4
33	0.0	0.0	0.0	0.08426733372519209	6.739752818544205E-4
34	0.0	0.0	0.0	0.11406521058051329	7.148222686334763E-4
35	0.0	0.0	0.0	0.1498063240121871	7.148222686334763E-4
36	0.0	0.0	0.0	0.2006199755653325	7.148222686334763E-4
37	0.0	0.0	0.0	0.2790257666877301	7.148222686334763E-4
38	0.0	0.0	0.0	0.38978237133913984	7.352457620230042E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5815	0.0	27.31753	1
GGTATCA	2445	0.0	24.386213	1
TCAACGC	8355	0.0	18.931223	4
CAACGCA	8530	0.0	18.465654	5
TATCAAC	8720	0.0	18.3154	2
AACGCAG	8600	0.0	18.315351	6
ATCAACG	8830	0.0	17.96267	3
CGGATCG	290	0.0	16.690685	26
ATACCGT	1060	0.0	16.18786	6
ATACGAA	1090	0.0	15.944309	40
CGCGAAA	85	0.0029822777	15.528943	14
ACCGTCG	1020	0.0	15.528785	8
TTAACGG	485	0.0	15.423633	35
TACCGTC	1105	0.0	15.329698	7
ACGCAGA	10370	0.0	15.253	7
CGTCGTA	1055	0.0	15.222445	10
CGCAATA	1160	0.0	15.17335	36
TAACGGC	495	0.0	15.112044	36
CGCAGAG	10555	0.0	14.985658	8
CTAGCGG	1175	0.0	14.979342	29
>>END_MODULE