FastQCFastQC Report
Thu 2 Feb 2017
SRR4063922_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063922_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114358
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64060.30297612797832724No Hit
GTCCTACAGTGGACATTTCTAAATT40500.19154750520016006No Hit
TATCAACGCAGAGTACTTTTTTTTT40370.19093266135630768No Hit
GTCCTAAAGTGTGTATTTCTCATTT38850.18374371795126465No Hit
CTGTAGGACGTGGAATATGGCAAGA36050.17050092746829063No Hit
CTTTAGGACGTGAAATATGGCGAGG33150.1567851801823532No Hit
GATATACACTGTTCTACAAATCCCG30530.14439371194471326No Hit
GTCCTACAGTGTGCATTTCTCATTT29080.13753583830174457No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28480.13469809748396439No Hit
TTGTAGAACAGTGTATATCAATGAG26720.1263740577518093No Hit
GTGTATATCAATGAGTTACAATGAG24890.11771894825757985No Hit
GGTATCAACGCAGAGTACTTTTTTT23950.11327315430972429No Hit
CTGTAGGACCTGGAATATGGCGAGA21450.10144923423564033No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21420.10130734719475132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1950.013.15556616
ATAACGA2000.012.8260712
TAACGAA2100.012.66772513
CCAACGA3500.012.48675619
GTAAGAC1301.9826984E-1012.4199973
CCGATAA2150.012.37253959
CCATTAG853.948835E-612.2931071
GCGTCCC2400.012.27243916
ATTTAGA5600.011.7043951
CGGCGTC2600.011.69356114
CGTCAAT3250.011.69328419
ATTGTCG658.029459E-411.6916236
GATAACG2200.011.659512511
CGATAAC2200.011.659512510
CGTGCGC907.4610034E-611.6115310
GATATAC11050.011.6053811
TCCAACG5250.011.58219218
TGTAGAA15050.011.4844382
ATTCCGA2400.011.4784956
CGTCTTA1251.8293576E-811.4014915
Produced by FastQC (version 0.11.5)