##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063922_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2114358
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26664973481312	32.0	32.0	32.0	32.0	32.0
2	30.86819592519337	32.0	32.0	32.0	32.0	32.0
3	30.892849271504637	32.0	32.0	32.0	32.0	32.0
4	30.922973782112585	32.0	32.0	32.0	32.0	32.0
5	30.83485720015248	32.0	32.0	32.0	32.0	32.0
6	34.51055544992854	36.0	36.0	36.0	32.0	36.0
7	34.43987158276886	36.0	36.0	36.0	32.0	36.0
8	34.40058495297391	36.0	36.0	36.0	32.0	36.0
9	34.53777553280948	36.0	36.0	36.0	32.0	36.0
10	34.231448032925364	36.0	36.0	36.0	32.0	36.0
11	34.51120198187819	36.0	36.0	36.0	32.0	36.0
12	34.32814263242081	36.0	36.0	36.0	32.0	36.0
13	34.409645386448275	36.0	36.0	36.0	32.0	36.0
14	34.31802466753501	36.0	36.0	36.0	32.0	36.0
15	34.250820816531544	36.0	36.0	36.0	32.0	36.0
16	34.247987805281795	36.0	36.0	36.0	32.0	36.0
17	34.18574716296862	36.0	36.0	36.0	32.0	36.0
18	34.18099631188285	36.0	36.0	36.0	32.0	36.0
19	34.179910403063246	36.0	36.0	36.0	32.0	36.0
20	34.14778292039475	36.0	36.0	36.0	32.0	36.0
21	34.13245817406513	36.0	36.0	36.0	32.0	36.0
22	34.09492810583638	36.0	36.0	36.0	32.0	36.0
23	34.048076531978026	36.0	36.0	36.0	32.0	36.0
24	34.02993343605955	36.0	36.0	36.0	32.0	36.0
25	33.642920451503485	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	11.0
4	12.0
5	35.0
6	80.0
7	44.0
8	123.0
9	142.0
10	207.0
11	72.0
12	124.0
13	120.0
14	222.0
15	418.0
16	630.0
17	841.0
18	1104.0
19	1478.0
20	2188.0
21	3292.0
22	5078.0
23	7651.0
24	11384.0
25	16490.0
26	22866.0
27	30272.0
28	41191.0
29	55007.0
30	72802.0
31	98377.0
32	138534.0
33	198780.0
34	432198.0
35	972583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.48158781633853	17.258326042964363	11.462967685646545	25.797118455050562
2	15.767781263125233	19.650438359170728	39.917218793632536	24.6645615840715
3	19.724770859159317	23.855099841035685	28.495476777068273	27.92465252273672
4	12.306995210146713	15.71139986974172	36.41641216481787	35.565192755293694
5	14.005664645065712	37.18473383830787	34.01029058266948	14.799310933956935
6	33.39816883846688	36.02580427876492	17.23747769702772	13.338549185740478
7	29.672577541199825	30.43152664056572	20.591869598702647	19.30402621953181
8	26.980167687766343	35.14516427411239	19.31853720551616	18.556130832605103
9	27.363502837263354	14.337643803257837	18.652025453981064	39.646827905497744
10	16.021244486215362	27.0546345604802	31.83506987084931	25.08905108245513
11	36.973062041916535	20.733435171724516	23.6040758071355	18.68942697922345
12	25.212241223781618	23.195937765922707	29.99953618820536	21.592284822090317
13	30.13926126454434	19.84495500334842	25.44924477089925	24.566538961207986
14	22.737749624445186	20.830773628716145	25.35238731699098	31.079089429847684
15	24.272227393771203	29.505119833492422	22.31908557128369	23.903567201452685
16	24.372086067123572	25.825437820559237	26.17150099696674	23.63097511535045
17	22.772011076231678	26.72247376115302	26.390622212189065	24.114892950426245
18	23.39811997101239	25.462563907400597	28.59241124912254	22.54690487246447
19	25.53222205340183	24.38255520545047	25.583909492076252	24.501313249071448
20	25.322627293585036	25.35216467849456	25.90935146979258	23.415856558127825
21	26.049265495220368	24.336635875337716	24.729400449468837	24.88469817997308
22	25.22748024828162	25.76321794792296	25.487917500749074	23.52138430304634
23	23.77055854095424	25.5339634859863	26.125915027853203	24.569562945206258
24	24.668428735608245	25.287172428417527	26.370352558944425	23.674046277029802
25	24.24344839468866	25.41952805342391	26.586188093222983	23.75083545866444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	362.0
1	362.0
2	691.5
3	1021.0
4	1021.0
5	1021.0
6	2510.0
7	3999.0
8	3999.0
9	3999.0
10	4102.5
11	4206.0
12	4206.0
13	4206.0
14	4606.5
15	5007.0
16	5007.0
17	5007.0
18	9357.5
19	13708.0
20	13708.0
21	13708.0
22	23883.5
23	34059.0
24	34059.0
25	34059.0
26	55324.0
27	76589.0
28	76589.0
29	76589.0
30	103266.5
31	129944.0
32	129944.0
33	129944.0
34	145164.5
35	160385.0
36	160385.0
37	160385.0
38	174239.5
39	188094.0
40	188094.0
41	188094.0
42	205193.5
43	222293.0
44	222293.0
45	222293.0
46	243370.0
47	264447.0
48	264447.0
49	264447.0
50	267391.5
51	270336.0
52	270336.0
53	270336.0
54	245272.5
55	220209.0
56	220209.0
57	220209.0
58	202921.5
59	185634.0
60	185634.0
61	185634.0
62	163024.5
63	140415.0
64	140415.0
65	140415.0
66	116116.5
67	91818.0
68	91818.0
69	91818.0
70	69965.0
71	48112.0
72	48112.0
73	48112.0
74	37075.0
75	26038.0
76	26038.0
77	26038.0
78	20739.5
79	15441.0
80	15441.0
81	15441.0
82	10687.0
83	5933.0
84	5933.0
85	5933.0
86	4602.5
87	3272.0
88	3272.0
89	3272.0
90	2305.0
91	1338.0
92	1338.0
93	1338.0
94	943.5
95	549.0
96	549.0
97	549.0
98	849.0
99	1149.0
100	1149.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03968107576862575
2	0.005202524832596939
3	0.0022228969739277833
4	0.004587680988744574
5	0.009080770616896477
6	0.013715747285937387
7	0.024309979672316608
8	0.03722170039321628
9	0.04984964703233795
10	0.058883121968938086
11	0.057038590437381
12	0.06772741418435288
13	0.06730175306168587
14	0.07018678955976235
15	0.06153168006553289
16	0.06853144074939059
17	0.06455860360449839
18	0.08186882259295729
19	0.07846353361162112
20	0.08423360660777408
21	0.0776595070465834
22	0.08309851028066202
23	0.0876861912694066
24	0.07992969970080753
25	0.08257825779740233
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2114358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.5235960702892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7260351884679	31.652985793365158
2	14.687440041296519	12.785004153279628
3	5.219616182830677	6.815293991504014
4	2.32053913132972	4.039928312691781
5	1.25282492697601	2.726372303424671
6	0.7623316957427385	1.9907650078251335
7	0.508476957709119	1.5491522002866855
8	0.3867323513225178	1.346558611701955
9	0.281840393558976	1.1040036700996987
>10	1.5972817366195036	12.819138255552806
>50	0.1292057583573194	3.890520113710949
>100	0.10647136668791957	9.622941143405486
>500	0.01486484844204642	4.460869590443915
>1k	0.006230120302916514	4.8917241726289005
>5k	1.0930035619151778E-4	0.3047426800791677
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6406	0.30297612797832724	No Hit
GTCCTACAGTGGACATTTCTAAATT	4050	0.19154750520016006	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4037	0.19093266135630768	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3885	0.18374371795126465	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3605	0.17050092746829063	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3315	0.1567851801823532	No Hit
GATATACACTGTTCTACAAATCCCG	3053	0.14439371194471326	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2908	0.13753583830174457	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2848	0.13469809748396439	No Hit
TTGTAGAACAGTGTATATCAATGAG	2672	0.1263740577518093	No Hit
GTGTATATCAATGAGTTACAATGAG	2489	0.11771894825757985	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2395	0.11327315430972429	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2145	0.10144923423564033	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2142	0.10130734719475132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	9.459136059267162E-5
13	0.0	0.0	0.0	0.0	9.459136059267162E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	195	0.0	13.155566	16
ATAACGA	200	0.0	12.82607	12
TAACGAA	210	0.0	12.667725	13
CCAACGA	350	0.0	12.486756	19
GTAAGAC	130	1.9826984E-10	12.419997	3
CCGATAA	215	0.0	12.3725395	9
CCATTAG	85	3.948835E-6	12.293107	1
GCGTCCC	240	0.0	12.272439	16
ATTTAGA	560	0.0	11.704395	1
CGGCGTC	260	0.0	11.693561	14
CGTCAAT	325	0.0	11.693284	19
ATTGTCG	65	8.029459E-4	11.691623	6
GATAACG	220	0.0	11.6595125	11
CGATAAC	220	0.0	11.6595125	10
CGTGCGC	90	7.4610034E-6	11.61153	10
GATATAC	1105	0.0	11.605381	1
TCCAACG	525	0.0	11.582192	18
TGTAGAA	1505	0.0	11.484438	2
ATTCCGA	240	0.0	11.478495	6
CGTCTTA	125	1.8293576E-8	11.40149	15
>>END_MODULE