##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063922_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2114358
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290948836478968	32.0	32.0	32.0	32.0	32.0
2	31.425833751900104	32.0	32.0	32.0	32.0	32.0
3	31.50177642575193	32.0	32.0	32.0	32.0	32.0
4	31.58665278065493	32.0	32.0	32.0	32.0	32.0
5	31.539193930261572	32.0	32.0	32.0	32.0	32.0
6	35.1391405807342	36.0	36.0	36.0	36.0	36.0
7	35.15344374036942	36.0	36.0	36.0	36.0	36.0
8	35.10021765472072	36.0	36.0	36.0	36.0	36.0
9	35.20389262367111	36.0	36.0	36.0	36.0	36.0
10	35.067017506023106	36.0	36.0	36.0	36.0	36.0
11	35.20564445566929	36.0	36.0	36.0	36.0	36.0
12	35.11505147188887	36.0	36.0	36.0	36.0	36.0
13	35.158810854169445	36.0	36.0	36.0	36.0	36.0
14	35.0994155199829	36.0	36.0	36.0	36.0	36.0
15	35.07838123912791	36.0	36.0	36.0	36.0	36.0
16	35.084993175233336	36.0	36.0	36.0	36.0	36.0
17	35.05611206806038	36.0	36.0	36.0	36.0	36.0
18	35.05039449326935	36.0	36.0	36.0	36.0	36.0
19	35.04610761280729	36.0	36.0	36.0	36.0	36.0
20	35.03160439244442	36.0	36.0	36.0	36.0	36.0
21	35.02214714821236	36.0	36.0	36.0	36.0	36.0
22	34.99911462486485	36.0	36.0	36.0	36.0	36.0
23	34.95935078165571	36.0	36.0	36.0	36.0	36.0
24	34.93250528056271	36.0	36.0	36.0	36.0	36.0
25	34.89780491288609	36.0	36.0	36.0	32.0	36.0
26	34.84607431664836	36.0	36.0	36.0	32.0	36.0
27	34.83233681334949	36.0	36.0	36.0	32.0	36.0
28	34.791502195938435	36.0	36.0	36.0	32.0	36.0
29	34.766370690299375	36.0	36.0	36.0	32.0	36.0
30	34.72589079049054	36.0	36.0	36.0	32.0	36.0
31	34.72766958102649	36.0	36.0	36.0	32.0	36.0
32	34.683635883800186	36.0	36.0	36.0	32.0	36.0
33	34.67180250459005	36.0	36.0	36.0	32.0	36.0
34	34.65579670046416	36.0	36.0	36.0	32.0	36.0
35	34.625008631461654	36.0	36.0	36.0	32.0	36.0
36	34.57830651195304	36.0	36.0	36.0	32.0	36.0
37	34.54987045713167	36.0	36.0	36.0	32.0	36.0
38	34.49558589415794	36.0	36.0	36.0	32.0	36.0
39	34.461982313307395	36.0	36.0	36.0	32.0	36.0
40	34.46413190197686	36.0	36.0	36.0	32.0	36.0
41	34.4523912223001	36.0	36.0	36.0	32.0	36.0
42	34.38148884909746	36.0	36.0	36.0	32.0	36.0
43	34.37800504928683	36.0	36.0	36.0	32.0	36.0
44	34.291447805906095	36.0	36.0	36.0	32.0	36.0
45	34.257275258021586	36.0	36.0	36.0	32.0	36.0
46	34.18216356927256	36.0	36.0	36.0	32.0	36.0
47	34.16287497197731	36.0	36.0	36.0	32.0	36.0
48	34.1016351062592	36.0	36.0	36.0	32.0	36.0
49	34.076236380026465	36.0	36.0	36.0	32.0	36.0
50	33.504988275400855	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	0.0
19	5.0
20	30.0
21	64.0
22	219.0
23	662.0
24	1771.0
25	4137.0
26	8180.0
27	15186.0
28	24845.0
29	37727.0
30	53682.0
31	75393.0
32	108363.0
33	174875.0
34	359918.0
35	1249298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51909312617186	18.13648326241484	12.02182044533915	26.322603166074153
2	15.122297748525787	20.39399379856107	39.394650265708385	25.089058187204756
3	19.01370347633367	24.273087666492934	28.809177649532668	27.904031207640728
4	12.007048948191366	16.272409875716413	36.48213784042248	35.23840333566974
5	13.546523341837096	37.57239786261361	33.88678738416105	14.994291411388232
6	33.55816176390603	36.297723227862136	16.945041816574623	13.199073191657213
7	29.66001451979445	30.50504763864157	20.640147941097876	19.1947899004661
8	26.995570286583447	35.334981114834854	19.163925881993492	18.505522716588203
9	27.282229503730722	14.156217365641034	18.50022891347449	40.061324217153754
10	15.876640406292463	27.3030956719807	31.79121228904163	25.0290516326852
11	37.21353584091996	20.61264961404342	23.50009955745411	18.673714987582514
12	25.136755718295046	23.26691746036851	29.916371619889574	21.67995520144687
13	30.221827458703544	19.797327400529525	25.518812909523795	24.462032231243136
14	22.813445619796873	20.714459357326163	25.404118704814806	31.06797631806216
15	24.323664711374466	29.558919377068687	22.23787780322401	23.879538108332838
16	24.451295012821955	25.901249655921323	26.057846838401733	23.58960849285499
17	22.874934140765188	26.73005233739982	26.327329619676515	24.06768390215848
18	23.508276069844666	25.35877473397249	28.64687901571595	22.486070180466896
19	25.64545688874385	24.29691324304575	25.662294174819273	24.39533569339113
20	25.456336687890012	25.28271260318833	26.010127046858166	23.250823662063492
21	26.095675377585064	24.182754292319466	24.894979941901987	24.826590388193484
22	25.269159971168527	25.66171448895077	25.609594599963298	23.459530939917403
23	23.72025774570881	25.434085091650697	26.267729003770455	24.577928158870037
24	24.75783326215303	25.155449738903084	26.450269089918454	23.636447909025428
25	24.398443556210143	25.212894816126514	26.661832931546837	23.726828696116506
26	22.970250105712445	26.516938113522396	27.351995482034557	23.160816298730598
27	23.806422317767087	25.64911384501866	26.631097488872808	23.913366348341444
28	23.11043926729504	26.26834694889152	26.137993272312425	24.483220511501017
29	23.60201296889263	25.00503715195172	25.91484611855516	25.478103760600483
30	23.11764282984528	25.87936038262015	26.526961628254558	24.476035159280013
31	24.730700646583326	24.924249949153104	25.78420104153364	24.560848362729935
32	23.879557881961542	26.14099287841659	25.69341670935265	24.286032530269214
33	23.410506739291556	25.68470133281747	26.82004859422498	24.084743333665998
34	24.231038462266113	25.914826796829182	26.698497833777928	23.155636907126777
35	24.233804636854757	26.00192595297307	26.671327044031106	23.092942366141063
36	23.04095370713729	26.241514488750312	26.567691108470754	24.149840695641643
37	24.05100590512542	25.830678746514746	25.686940851785373	24.43137449657446
38	23.99384158397109	26.305234180627952	25.646491784048663	24.054432451352298
39	24.964845606824184	24.984805450645503	25.702271969426437	24.348076973103876
40	25.747368142247552	25.42219926727037	25.947908655688018	22.882523934794065
41	24.26777154235633	24.94862280745318	27.47460701473089	23.308998635459606
42	24.944166181874937	26.690456933940627	26.018417356016855	22.34695952816758
43	23.481500470126623	25.91201726887648	26.856905564142487	23.749576696854408
44	23.053202025428533	26.51233163601691	25.891847867728508	24.542618470826053
45	23.25703156186091	26.6424635013366	25.995211708328526	24.105293228473972
46	23.54474970049571	25.230203275084694	25.868893506065998	25.356153518353597
47	24.651443274101474	25.528900954930496	26.02802832156421	23.79162744940382
48	24.243057736475876	25.85267152754392	25.08466175029419	24.819608985686013
49	24.220870188701376	26.000853218914976	25.576466671017894	24.201809921365754
50	23.768049453018214	27.00444112319269	24.907795850222055	24.31971357356704
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	85.5
2	79.0
3	198.0
4	317.0
5	350.5
6	384.0
7	364.5
8	345.0
9	472.5
10	600.0
11	959.5
12	1319.0
13	2503.0
14	3687.0
15	4881.5
16	6076.0
17	6593.5
18	7111.0
19	7201.5
20	7292.0
21	7913.5
22	8535.0
23	9669.5
24	10804.0
25	12927.0
26	15050.0
27	21399.0
28	27748.0
29	34303.5
30	40859.0
31	46942.5
32	53026.0
33	63909.0
34	74792.0
35	87799.5
36	100807.0
37	106001.5
38	111196.0
39	110170.0
40	109144.0
41	108467.0
42	107790.0
43	107012.5
44	106235.0
45	113701.0
46	121167.0
47	127715.5
48	134264.0
49	139582.5
50	144901.0
51	140863.5
52	136826.0
53	135638.5
54	134451.0
55	134537.0
56	134623.0
57	130584.5
58	126546.0
59	115976.5
60	105407.0
61	93306.0
62	81205.0
63	70975.0
64	60745.0
65	51888.0
66	43031.0
67	37526.5
68	32022.0
69	29204.0
70	26386.0
71	20368.0
72	14350.0
73	12045.0
74	9740.0
75	7673.0
76	5606.0
77	4832.0
78	4058.0
79	3259.0
80	2460.0
81	1979.0
82	1498.0
83	1197.0
84	896.0
85	651.5
86	407.0
87	299.5
88	192.0
89	145.5
90	99.0
91	80.0
92	61.0
93	52.5
94	44.0
95	39.5
96	35.0
97	33.0
98	31.0
99	25.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009175361977489149
2	4.729568029633581E-4
3	1.8918272118534328E-4
4	0.0
5	0.0
6	2.3647840148167905E-4
7	1.4188704088900745E-4
8	0.0
9	0.001040504966519388
10	8.986179256303804E-4
11	4.729568029633582E-5
12	1.8918272118534328E-4
13	5.675481635560298E-4
14	1.8918272118534328E-4
15	0.004540385308448238
16	7.567308847413731E-4
17	0.0
18	4.256611226670223E-4
19	1.4188704088900745E-4
20	9.459136059267162E-4
21	0.0
22	9.459136059267164E-5
23	0.0012296876877047312
24	4.256611226670223E-4
25	0.0024120796951131265
26	0.0062430297991163276
27	0.007945674289784417
28	0.005202524832596939
29	0.0032161062601508356
30	0.004445793947855567
31	0.007945674289784417
32	0.003830950104003201
33	0.005439003234078619
34	0.0036890630631141934
35	0.0037836544237068647
36	0.007851082929191745
37	0.004398498267559231
38	0.008418631092747774
39	0.005344411873485947
40	0.0034052889813361784
41	0.004398498267559231
42	0.002270192654224119
43	0.0016080531300754178
44	0.0018918272118534324
45	0.0016080531300754178
46	0.00146616608918641
47	0.0032161062601508356
48	0.00293233217837282
49	5.675481635560298E-4
50	0.0013242790482974028
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2114358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.10166381733149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34243885524563	36.50337440030793
2	14.224784916047295	13.969212136432043
3	4.837524158818932	7.125914548636396
4	2.146728371850112	4.216317392868463
5	1.1650923483541982	2.8603986402516557
6	0.6989479934834631	2.0591705641094045
7	0.4682336006665907	1.6093734193536706
8	0.33058736911135594	1.2985911888289503
9	0.24009267798377984	1.0610054963426145
>10	1.3489500529676723	12.012010678999735
>50	0.10534191012584086	3.6050939464845713
>100	0.07872694395593004	8.098802086918312
>500	0.008659080028208628	2.957404291184373
>1k	0.0038917213609926398	2.6233312092819343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	4.729568029633581E-5
13	0.0	0.0	0.0	0.0	4.729568029633581E-5
14	0.0	0.0	0.0	0.0	4.729568029633581E-5
15	0.0	0.0	0.0	0.0	4.729568029633581E-5
16	0.0	0.0	0.0	0.0	4.729568029633581E-5
17	0.0	0.0	0.0	0.0	4.729568029633581E-5
18	0.0	0.0	0.0	0.0	4.729568029633581E-5
19	0.0	0.0	0.0	0.0	4.729568029633581E-5
20	0.0	0.0	0.0	4.729568029633581E-5	4.729568029633581E-5
21	0.0	0.0	0.0	9.459136059267162E-5	4.729568029633581E-5
22	0.0	0.0	0.0	3.310697620743507E-4	4.729568029633581E-5
23	0.0	0.0	0.0	9.93209286223052E-4	4.729568029633581E-5
24	0.0	0.0	0.0	0.002081009933038776	4.729568029633581E-5
25	0.0	0.0	0.0	0.0025066710557057983	4.729568029633581E-5
26	0.0	0.0	0.0	0.0028850364980764847	9.459136059267162E-5
27	0.0	0.0	0.0	0.003878245784299537	9.459136059267162E-5
28	0.0	0.0	0.0	0.006195734118819992	9.459136059267162E-5
29	0.0	0.0	0.0	0.012817129360307006	9.459136059267162E-5
30	0.0	0.0	0.0	0.023931614229945924	9.459136059267162E-5
31	0.0	0.0	0.0	0.04970775999144894	9.459136059267162E-5
32	0.0	0.0	0.0	0.07775409840717608	9.459136059267162E-5
33	0.0	0.0	0.0	0.10291540032482674	9.459136059267162E-5
34	0.0	0.0	0.0	0.1315292869041099	1.4188704088900745E-4
35	0.0	0.0	0.0	0.16804155209288116	1.4188704088900745E-4
36	0.0	0.0	0.0	0.2175128336828484	1.4188704088900745E-4
37	0.0	0.0	0.0	0.29398994872202344	1.4188704088900745E-4
38	0.0	0.0	0.0	0.39293251190195794	1.4188704088900745E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAT	60	2.871748E-4	22.002283	21
TGTAGGA	2655	0.0	21.540888	2
GTAGGAC	2745	0.0	20.754494	3
CTGTAGG	2850	0.0	20.146599	1
GTATCAA	4480	0.0	20.13316	1
GTCCTAC	2410	0.0	19.260675	1
GATATAC	1825	0.0	19.166391	1
TTAACGG	185	1.8189894E-12	19.029001	35
TAGGACC	1800	0.0	18.575312	4
GTCCTAA	1380	0.0	18.492048	1
ATACACT	1845	0.0	18.47993	4
ACTGTTC	1755	0.0	18.424906	8
CGGATAC	60	0.0074104685	18.3348	25
TAGCGAT	60	0.007413502	18.333502	38
TAGGACG	2600	0.0	18.274496	4
TTAGGAC	1575	0.0	17.876568	3
TCCTACA	2675	0.0	17.597246	2
TGGCGAG	3175	0.0	17.46075	18
AGGACGT	2670	0.0	17.383461	5
TAGGACA	1015	0.0	17.337599	4
>>END_MODULE