FastQCFastQC Report
Thu 2 Feb 2017
SRR4063921_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063921_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9450162
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT203110.2149275324592319No Hit
GCGCAAGACGGACCAGAGCGAAAGC145460.1539232872410018No Hit
TATCAACGCAGAGTACTTTTTTTTT125230.13251624681143032No Hit
GTCTTGCGCCGGTCCAAGAATTTCA111740.11824135924865627No Hit
GTATCTGATCGTCTTCGAACCTCCG109040.1153842653702656No Hit
GAATAGGACCGCGGTTCTATTTTGT107800.11407211855204175No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG105430.1115642250365655No Hit
GAACTACGACGGTATCTGATCGTCT104800.11089756979827435No Hit
GGGTAGGCACACGCTGAGCCAGTCA102080.10801931226152525No Hit
ATCAGATACCGTCGTAGTTCCGACC100530.10637912873874543No Hit
GTGCATGGCCGTTCTTAGTTGGTGG98220.10393472619834453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37250.014.6091681
GTATCAA118250.013.2358911
AACCGCG8050.012.8633547
AAGACGG27400.012.4797695
CGAACGA10250.012.42181516
CAAGACG27700.012.4126644
TCGCGTA12600.012.3659319
CGGTCCA21100.012.02215610
CCGTCGT17450.011.9779019
GCGTAAC12950.011.88498911
GCGTTAT9200.011.8715421
CGCGGTC8600.011.71010810
CGCGTAA13250.011.68759710
CGTCGTA18050.011.68501610
CGACCAT18100.011.65273610
CGTTATT9500.011.4948872
CGGACCA29150.011.4724879
CGCATCG13350.011.38691413
CGCAAGA29650.011.27323152
GATAACG11750.011.23907211
Produced by FastQC (version 0.11.5)