##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063921_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9450162
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.299654122331447	32.0	32.0	32.0	32.0	32.0
2	31.008377634161192	32.0	32.0	32.0	32.0	32.0
3	31.017098754497543	32.0	32.0	32.0	32.0	32.0
4	31.042098114296877	32.0	32.0	32.0	32.0	32.0
5	30.99203812590726	32.0	32.0	32.0	32.0	32.0
6	34.616912493140326	36.0	36.0	36.0	32.0	36.0
7	34.57136745380661	36.0	36.0	36.0	32.0	36.0
8	34.54767801864137	36.0	36.0	36.0	32.0	36.0
9	34.64586988032586	36.0	36.0	36.0	32.0	36.0
10	34.43020341873505	36.0	36.0	36.0	32.0	36.0
11	34.59917554852499	36.0	36.0	36.0	32.0	36.0
12	34.47057150977941	36.0	36.0	36.0	32.0	36.0
13	34.53263256227777	36.0	36.0	36.0	32.0	36.0
14	34.44916669153397	36.0	36.0	36.0	32.0	36.0
15	34.4027572225746	36.0	36.0	36.0	32.0	36.0
16	34.39603204685803	36.0	36.0	36.0	32.0	36.0
17	34.328241780405456	36.0	36.0	36.0	32.0	36.0
18	34.31319304367481	36.0	36.0	36.0	32.0	36.0
19	34.33702152407546	36.0	36.0	36.0	32.0	36.0
20	34.31734694071911	36.0	36.0	36.0	32.0	36.0
21	34.298324515495075	36.0	36.0	36.0	32.0	36.0
22	34.2846105706971	36.0	36.0	36.0	32.0	36.0
23	34.235420726120886	36.0	36.0	36.0	32.0	36.0
24	34.222975754278075	36.0	36.0	36.0	32.0	36.0
25	33.82350503620996	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	20.0
4	76.0
5	112.0
6	364.0
7	121.0
8	520.0
9	602.0
10	817.0
11	267.0
12	606.0
13	502.0
14	713.0
15	1367.0
16	2365.0
17	3215.0
18	4202.0
19	5640.0
20	8257.0
21	11789.0
22	18013.0
23	27369.0
24	41057.0
25	60451.0
26	86971.0
27	117580.0
28	162431.0
29	224180.0
30	300708.0
31	416366.0
32	598241.0
33	865500.0
34	1938933.0
35	4550807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.126238797128494	17.169825062547226	11.572935983863589	26.131000156460694
2	17.082430080706736	19.162243661005256	37.11898171836085	26.63634453992716
3	19.095578633696014	22.725475713278914	28.165009174583755	30.013936478441316
4	12.778422176184861	14.915067631497047	35.4301614222419	36.87634877007619
5	15.063637703795743	35.733332373831814	33.369085347766216	15.833944574606235
6	35.0179549487471	35.037883137418675	16.32235067537298	13.621811238461257
7	31.218048324644947	29.957143569307426	20.15852571592233	18.666282390125293
8	28.417873749243398	32.484601797714845	19.06415259553364	20.03337185750812
9	27.032803739198346	14.534038433096425	18.53218107234634	39.90097675535889
10	15.913710109131321	25.972178459015616	30.942676410897548	27.17143502095552
11	37.50441896780323	21.210039640748374	22.14897302605939	19.136568365389003
12	25.06020345766857	23.39851073010226	28.078841831913152	23.462443980316014
13	28.8678299898328	19.230939463330206	25.460038834852767	26.44119171198423
14	23.781557852025077	19.540277838810287	24.77084571999155	31.907318589173084
15	25.298500256444246	27.173582049665058	21.902678667099792	25.625239026790908
16	26.171555806203735	25.83481143871971	23.25168925447474	24.741943500601813
17	24.194421588214947	25.987111168947287	24.812148617457236	25.006318625380537
18	25.065451443934784	24.91166358392007	25.762347030216848	24.2605379419283
19	25.425440032599244	24.783843635459473	25.493934024655296	24.29678230728599
20	25.870797452091153	24.037121068030793	24.782234448449408	25.309847031428646
21	26.941804659071018	24.099668195560135	24.416721300260832	24.541805845108016
22	26.061933665118936	23.92884771485712	25.078564775764082	24.930653844259858
23	24.400232611116976	24.008328842052244	25.526909613466703	26.064528933364073
24	24.57283043908167	24.825241956586915	25.33564589655756	25.266281707773864
25	24.813702269926925	24.256633217954604	25.326557811415345	25.603106700703126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	307.0
1	307.0
2	1115.0
3	1923.0
4	1923.0
5	1923.0
6	5332.5
7	8742.0
8	8742.0
9	8742.0
10	10472.0
11	12202.0
12	12202.0
13	12202.0
14	16984.5
15	21767.0
16	21767.0
17	21767.0
18	39123.0
19	56479.0
20	56479.0
21	56479.0
22	89207.5
23	121936.0
24	121936.0
25	121936.0
26	182046.5
27	242157.0
28	242157.0
29	242157.0
30	304491.5
31	366826.0
32	366826.0
33	366826.0
34	468699.0
35	570572.0
36	570572.0
37	570572.0
38	670270.5
39	769969.0
40	769969.0
41	769969.0
42	881488.5
43	993008.0
44	993008.0
45	993008.0
46	1108577.5
47	1224147.0
48	1224147.0
49	1224147.0
50	1260502.0
51	1296857.0
52	1296857.0
53	1296857.0
54	1208263.0
55	1119669.0
56	1119669.0
57	1119669.0
58	1030953.5
59	942238.0
60	942238.0
61	942238.0
62	829509.0
63	716780.0
64	716780.0
65	716780.0
66	592138.5
67	467497.0
68	467497.0
69	467497.0
70	354307.5
71	241118.0
72	241118.0
73	241118.0
74	185930.5
75	130743.0
76	130743.0
77	130743.0
78	106673.0
79	82603.0
80	82603.0
81	82603.0
82	56929.5
83	31256.0
84	31256.0
85	31256.0
86	24237.0
87	17218.0
88	17218.0
89	17218.0
90	12033.0
91	6848.0
92	6848.0
93	6848.0
94	4636.5
95	2425.0
96	2425.0
97	2425.0
98	3650.0
99	4875.0
100	4875.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03915276796313121
2	0.005470805685659145
3	0.0015026197434499006
4	0.0036930583835494035
5	0.008581863464351193
6	0.013068559036342445
7	0.022274750422267894
8	0.034708399707856864
9	0.04762881313569016
10	0.05707838659273777
11	0.05378743771799891
12	0.06282431983705676
13	0.0642105394595352
14	0.06557559542365517
15	0.059406389012167195
16	0.06563908639873052
17	0.06202010081943569
18	0.07707804374147237
19	0.07405164059621412
20	0.07870764543507296
21	0.0735331309664321
22	0.07742724410438678
23	0.08246419479369771
24	0.07670767972019951
25	0.07774469897976352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9450162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.019128022949392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.19021408191666	13.39487987634472
2	14.800950203553143	6.814099351937773
3	7.673539654783306	5.299145751079074
4	4.584777142472117	4.221502879970309
5	3.0127301249269998	3.467521022214546
6	2.159871682560454	2.9831017664401314
7	1.5507806538188589	2.4988332884038633
8	1.2285713095387474	2.262451220767601
9	0.897821950177502	1.8600370571655005
>10	5.198067822096611	21.435230870196637
>50	0.3933355218030724	6.292483897640959
>100	0.2638765542806745	11.646335712107083
>500	0.02224996221348478	3.544472591523553
>1k	0.020593216785396936	9.627095861936429
>5k	0.002160449060236628	3.366959916699124
>10k+	4.596700128163037E-4	1.285848935572721
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	20311	0.2149275324592319	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	14546	0.1539232872410018	No Hit
TATCAACGCAGAGTACTTTTTTTTT	12523	0.13251624681143032	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	11174	0.11824135924865627	No Hit
GTATCTGATCGTCTTCGAACCTCCG	10904	0.1153842653702656	No Hit
GAATAGGACCGCGGTTCTATTTTGT	10780	0.11407211855204175	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	10543	0.1115642250365655	No Hit
GAACTACGACGGTATCTGATCGTCT	10480	0.11089756979827435	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	10208	0.10801931226152525	No Hit
ATCAGATACCGTCGTAGTTCCGACC	10053	0.10637912873874543	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	9822	0.10393472619834453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.407280425457257E-5
2	0.0	0.0	0.0	0.0	7.407280425457257E-5
3	0.0	0.0	0.0	0.0	7.407280425457257E-5
4	0.0	0.0	0.0	0.0	7.407280425457257E-5
5	0.0	0.0	0.0	0.0	9.523646261302187E-5
6	0.0	0.0	0.0	0.0	9.523646261302187E-5
7	0.0	0.0	0.0	1.0581829179224652E-5	9.523646261302187E-5
8	0.0	0.0	0.0	2.1163658358449304E-5	9.523646261302187E-5
9	0.0	0.0	0.0	2.1163658358449304E-5	9.523646261302187E-5
10	0.0	0.0	0.0	2.1163658358449304E-5	1.1640012097147117E-4
11	0.0	0.0	0.0	3.174548753767396E-5	1.1640012097147117E-4
12	0.0	0.0	0.0	4.232731671689861E-5	4.126913379897614E-4
13	0.0	0.0	0.0	4.232731671689861E-5	4.5501865470666005E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3725	0.0	14.609168	1
GTATCAA	11825	0.0	13.235891	1
AACCGCG	805	0.0	12.863354	7
AAGACGG	2740	0.0	12.479769	5
CGAACGA	1025	0.0	12.421815	16
CAAGACG	2770	0.0	12.412664	4
TCGCGTA	1260	0.0	12.365931	9
CGGTCCA	2110	0.0	12.022156	10
CCGTCGT	1745	0.0	11.977901	9
GCGTAAC	1295	0.0	11.884989	11
GCGTTAT	920	0.0	11.871542	1
CGCGGTC	860	0.0	11.710108	10
CGCGTAA	1325	0.0	11.687597	10
CGTCGTA	1805	0.0	11.685016	10
CGACCAT	1810	0.0	11.652736	10
CGTTATT	950	0.0	11.494887	2
CGGACCA	2915	0.0	11.472487	9
CGCATCG	1335	0.0	11.386914	13
CGCAAGA	2965	0.0	11.2732315	2
GATAACG	1175	0.0	11.239072	11
>>END_MODULE