FastQCFastQC Report
Thu 2 Feb 2017
SRR4063921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9450162
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT139390.14750011692921242No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT109950.11634721182557504No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC108510.1148234284237667No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG104250.110315569193417No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA102930.10891876774175935No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA100220.10605109203418947No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT99340.1051198910664177No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC99260.10503523643298389No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA96580.10219930621295169No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC96160.10175486938742426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121600.027.9704991
GGTATCA47150.026.8293691
TCAACGC179150.018.603414
ATCAACG183000.018.2960783
CAACGCA185900.017.951595
CGTCGTA31050.017.92589810
AACGCAG188150.017.7603936
TATCAAC191400.017.7455962
ACCGTCG31400.017.3750348
CCGTCGT31800.016.9499289
ATACCGT32600.016.8027676
CGCAATA30500.016.73519536
TACCGTC33550.016.3269797
TCTAGCG31000.016.32341828
CTAGCGG31100.016.2707629
TAGATCG4750.016.2095575
TATCGAG3200.015.81247510
GTCGTAG34450.015.70953411
GGCGTTA33600.015.64837142
CGGTCCA34750.015.51076810
Produced by FastQC (version 0.11.5)