##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063921_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9450162
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.363802334817116	32.0	32.0	32.0	32.0	32.0
2	31.39507788332094	32.0	32.0	32.0	32.0	32.0
3	31.467984040908505	32.0	32.0	32.0	32.0	32.0
4	31.55190535358018	32.0	32.0	32.0	32.0	32.0
5	31.492425844128388	32.0	32.0	32.0	32.0	32.0
6	35.091403829902596	36.0	36.0	36.0	36.0	36.0
7	35.10949494834057	36.0	36.0	36.0	36.0	36.0
8	35.048496523128385	36.0	36.0	36.0	36.0	36.0
9	35.1661931298109	36.0	36.0	36.0	36.0	36.0
10	35.01605951305385	36.0	36.0	36.0	36.0	36.0
11	35.16542922756245	36.0	36.0	36.0	36.0	36.0
12	35.062705380077084	36.0	36.0	36.0	36.0	36.0
13	35.112426220841506	36.0	36.0	36.0	36.0	36.0
14	35.05526212143241	36.0	36.0	36.0	36.0	36.0
15	35.02136270256531	36.0	36.0	36.0	36.0	36.0
16	35.033914762519416	36.0	36.0	36.0	36.0	36.0
17	34.99474728581372	36.0	36.0	36.0	36.0	36.0
18	34.99619054149548	36.0	36.0	36.0	36.0	36.0
19	35.00089765656927	36.0	36.0	36.0	36.0	36.0
20	34.991615805104715	36.0	36.0	36.0	36.0	36.0
21	34.988305808937454	36.0	36.0	36.0	36.0	36.0
22	34.972952209708154	36.0	36.0	36.0	36.0	36.0
23	34.91377703366355	36.0	36.0	36.0	32.0	36.0
24	34.88830625337428	36.0	36.0	36.0	32.0	36.0
25	34.85795915456264	36.0	36.0	36.0	32.0	36.0
26	34.79849879822166	36.0	36.0	36.0	32.0	36.0
27	34.79919328367069	36.0	36.0	36.0	32.0	36.0
28	34.77678816511294	36.0	36.0	36.0	32.0	36.0
29	34.74727925299058	36.0	36.0	36.0	32.0	36.0
30	34.73370223706218	36.0	36.0	36.0	32.0	36.0
31	34.73234342437728	36.0	36.0	36.0	32.0	36.0
32	34.697568147509	36.0	36.0	36.0	32.0	36.0
33	34.67255968733658	36.0	36.0	36.0	32.0	36.0
34	34.668065267029284	36.0	36.0	36.0	32.0	36.0
35	34.63097733139389	36.0	36.0	36.0	32.0	36.0
36	34.608468087637014	36.0	36.0	36.0	32.0	36.0
37	34.60385726720875	36.0	36.0	36.0	32.0	36.0
38	34.56527105037988	36.0	36.0	36.0	32.0	36.0
39	34.57012842742802	36.0	36.0	36.0	32.0	36.0
40	34.54696893026807	36.0	36.0	36.0	32.0	36.0
41	34.53773808322016	36.0	36.0	36.0	32.0	36.0
42	34.46390252357579	36.0	36.0	36.0	32.0	36.0
43	34.460108726178454	36.0	36.0	36.0	32.0	36.0
44	34.39962140331563	36.0	36.0	36.0	32.0	36.0
45	34.38107579531441	36.0	36.0	36.0	32.0	36.0
46	34.35830359310243	36.0	36.0	36.0	32.0	36.0
47	34.34075098395139	36.0	36.0	36.0	32.0	36.0
48	34.294082048540545	36.0	36.0	36.0	32.0	36.0
49	34.28622736837739	36.0	36.0	36.0	32.0	36.0
50	33.67116997571047	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	5.0
19	28.0
20	79.0
21	319.0
22	816.0
23	2572.0
24	6517.0
25	15025.0
26	31302.0
27	58427.0
28	98601.0
29	156036.0
30	232885.0
31	337980.0
32	498367.0
33	819042.0
34	1708206.0
35	5483951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.162731267034225	18.10729363836146	12.113098053857204	26.616877040747106
2	16.24201742349536	19.906428632123234	36.638143903676074	27.213410040705334
3	18.326675225277274	23.253685768622066	28.50216272111331	29.917476284987345
4	12.402073107318161	15.546484811583126	35.47385748519443	36.577584595904284
5	14.64592882111439	36.15572939384531	33.139029785944416	16.059311999095886
6	35.10949730802959	35.39914377997111	16.07265965661253	13.418699255386771
7	31.192014184739385	30.11488910870889	20.090969926845652	18.602126779706072
8	28.434753651286997	32.688660013305594	18.892524688203725	19.98406164720368
9	27.156805820413034	14.354430468153028	18.344419516889378	40.14434419454456
10	15.816553933934951	26.187355027682308	30.963505070856545	27.032585967526202
11	37.74005942756525	21.122774640852644	22.07855740008635	19.058608531495764
12	25.036917407334002	23.45152441511769	27.992055998270505	23.519502179277808
13	28.93402245303871	19.193621163066222	25.448703106964523	26.423653276930548
14	23.76607171276967	19.485343292123382	24.71818418851607	32.03040080659088
15	25.34303023889521	27.271843170454247	21.761295272361703	25.623831318288843
16	26.32443656116013	25.882620850120645	23.126902229755597	24.666040358963627
17	24.327934730424168	26.083936559387716	24.636321010827224	24.951807699360888
18	25.146027045333646	25.00262165622431	25.60236982908071	24.24898146936133
19	25.55872093540043	24.855018266365125	25.324745961865602	24.26151483636884
20	26.027965203758143	24.020769725106643	24.62603309742404	25.325231973711183
21	27.087958915413303	24.136580939035753	24.254811716455233	24.52064842909571
22	26.164023605122967	23.965032520111844	24.9318741116545	24.939069763110687
23	24.477560862873773	24.009988893234524	25.427276082697997	26.08517416119371
24	24.746894721643187	24.834195209605298	25.162815410049333	25.25609465870218
25	25.02914028256365	24.20068323975815	25.230135480753024	25.54004099692518
26	24.778497438798095	25.288335618246784	25.389249248518336	24.543917694436786
27	25.525486595958018	24.543304857625873	24.716815306508494	25.21439323990762
28	24.80013816197587	24.34883655467885	25.628358676785844	25.222666606559436
29	24.632349323037673	24.75626551928664	25.612758182992795	24.998626974682896
30	24.64037701430936	24.910223224192357	25.67178213950372	24.777617621994562
31	25.281327173428085	24.670860752785124	24.659664388449922	25.38814768533687
32	25.10078494583603	24.72534938216839	24.409533976505806	25.76433169548978
33	24.69919558675922	24.431653975848025	25.052422049483873	25.81672838790888
34	25.49709086314517	24.54604195057674	25.117625807391036	24.83924137888706
35	25.925390155789295	24.284776556398235	25.239571850602534	24.550261437209937
36	24.702681171352584	24.916725329680496	24.937065133512505	25.44352836545442
37	25.879676137506635	24.673430045652754	24.761071991583325	24.685821825257285
38	24.946758591963185	24.204013964043476	25.288028503784147	25.56119894020919
39	25.70498478106759	24.54114360570441	24.34740288704148	25.40646872618652
40	25.648782583574693	24.703124785051315	24.971010367272562	24.677082264101426
41	24.573913871648756	25.150356883971703	25.567083075392734	24.70864616898681
42	26.180051638155344	24.88885436797863	24.823065931611136	24.10802806225489
43	25.475609550910466	24.02562488948449	25.074087058550386	25.424678501054654
44	24.926349518097275	24.687250001058196	24.71332396476629	25.67307651607824
45	25.186165780162952	25.077647778396823	24.703480247977655	25.032706193462573
46	24.851030093661812	24.723930411892493	24.75677675376344	25.66826274068226
47	25.014486833806703	24.59288716565996	25.284636125443917	25.10798987508942
48	26.119174286277254	25.134386840661087	24.13048819899517	24.61595067406649
49	25.097673290528267	25.0573775357728	24.52964917825972	25.315299995439215
50	25.300353162451554	25.205813900086373	24.46518227114758	25.0286506663145
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	74.5
2	115.0
3	404.5
4	694.0
5	815.5
6	937.0
7	963.0
8	989.0
9	1308.0
10	1627.0
11	2584.5
12	3542.0
13	6380.5
14	9219.0
15	12293.0
16	15367.0
17	15556.5
18	15746.0
19	15974.5
20	16203.0
21	19293.0
22	22383.0
23	27121.5
24	31860.0
25	41018.0
26	50176.0
27	62954.5
28	75733.0
29	87177.5
30	98622.0
31	113068.0
32	127514.0
33	147495.0
34	167476.0
35	197698.5
36	227921.0
37	277661.5
38	327402.0
39	370954.5
40	414507.0
41	457578.0
42	500649.0
43	519402.0
44	538155.0
45	589057.5
46	639960.0
47	685429.0
48	730898.0
49	750943.0
50	770988.0
51	741938.5
52	712889.0
53	704545.5
54	696202.0
55	693883.5
56	691565.0
57	668218.5
58	644872.0
59	588695.5
60	532519.0
61	468838.0
62	405157.0
63	352254.5
64	299352.0
65	254438.0
66	209524.0
67	182840.0
68	156156.0
69	142131.0
70	128106.0
71	97592.0
72	67078.0
73	57278.5
74	47479.0
75	35787.0
76	24095.0
77	20970.5
78	17846.0
79	14952.5
80	12059.0
81	9742.0
82	7425.0
83	6102.5
84	4780.0
85	3407.0
86	2034.0
87	1458.5
88	883.0
89	703.0
90	523.0
91	433.5
92	344.0
93	263.0
94	182.0
95	183.5
96	185.0
97	144.5
98	104.0
99	95.0
100	86.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008507790660096621
2	5.18509629782008E-4
3	3.174548753767396E-4
4	0.0
5	0.0
6	2.222184127637177E-4
7	6.349097507534791E-5
8	1.0581829179224652E-5
9	0.0011745830388939366
10	8.677099926964215E-4
11	2.1163658358449304E-5
12	1.3756377932992047E-4
13	4.021095088105368E-4
14	1.9047292522604374E-4
15	0.004359713621840557
16	7.195643841872764E-4
17	1.0581829179224652E-5
18	3.068730461975149E-4
19	6.349097507534791E-5
20	6.454915799327037E-4
21	0.0
22	1.0581829179224653E-4
23	6.984007258288271E-4
24	4.5501865470666E-4
25	0.0019576383981565605
26	0.005259169102074652
27	0.006211533728204871
28	0.0040634224048222665
29	0.002402075223683996
30	0.003544912775040259
31	0.007047498233363619
32	0.0035766582625779324
33	0.0044126227677366795
34	0.0029629121701829028
35	0.0029946576577205767
36	0.007513098717249503
37	0.0036930583835494035
38	0.00793637188441849
39	0.004433786426095129
40	0.002454984369580119
41	0.0033015307039180914
42	0.001830656448005865
43	0.0013862196224784294
44	0.0012909831598654075
45	0.0012380740139692843
46	0.0010476010887432406
47	0.0021375294942033796
48	0.002158693152561829
49	3.7036402127286285E-4
50	0.0012698195015069583
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9450162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.437534259418985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.56078928992009	14.310812945310387
2	14.654039942820809	7.162172062831555
3	7.76512161002725	5.692812761207869
4	4.566956575180879	4.464206310690462
5	3.0043270681832643	3.6709172827614163
6	2.1178617226011243	3.105319104166625
7	1.5872796089167012	2.715243987552534
8	1.1908803003119417	2.32817425101922
9	0.9091265956451926	1.999513109450551
>10	5.010078565555834	21.862375172527926
>50	0.3558726594587599	6.032860150794187
>100	0.23918325168978982	11.154270459451453
>500	0.018610662690549732	3.1094073659915034
>1k	0.0178675006284806	8.739121607434708
>5k	0.0017431707560338725	2.9443222182918545
>10k+	2.614756134050809E-4	0.7084712105178341
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	13939	0.14750011692921242	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	10995	0.11634721182557504	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	10851	0.1148234284237667	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	10425	0.110315569193417	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	10293	0.10891876774175935	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	10022	0.10605109203418947	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	9934	0.1051198910664177	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	9926	0.10503523643298389	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	9658	0.10219930621295169	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	9616	0.10175486938742426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.523646261302187E-5
2	0.0	0.0	0.0	0.0	9.523646261302187E-5
3	0.0	0.0	0.0	0.0	9.523646261302187E-5
4	0.0	0.0	0.0	1.0581829179224652E-5	9.523646261302187E-5
5	0.0	0.0	0.0	1.0581829179224652E-5	9.523646261302187E-5
6	0.0	0.0	0.0	1.0581829179224652E-5	1.2698195015069583E-4
7	0.0	0.0	0.0	1.0581829179224652E-5	1.2698195015069583E-4
8	0.0	0.0	0.0	2.1163658358449304E-5	1.2698195015069583E-4
9	0.0	0.0	0.0	2.1163658358449304E-5	1.2698195015069583E-4
10	0.0	0.0	0.0	2.1163658358449304E-5	1.3756377932992047E-4
11	0.0	0.0	0.0	2.1163658358449304E-5	1.3756377932992047E-4
12	0.0	0.0	0.0	3.174548753767396E-5	3.174548753767396E-4
13	0.0	0.0	0.0	3.174548753767396E-5	3.7036402127286285E-4
14	0.0	0.0	0.0	3.174548753767396E-5	3.7036402127286285E-4
15	0.0	0.0	0.0	3.174548753767396E-5	4.656004838858847E-4
16	0.0	0.0	0.0	3.174548753767396E-5	4.7618231306510933E-4
17	0.0	0.0	0.0	3.174548753767396E-5	4.973459714235586E-4
18	0.0	0.0	0.0	5.2909145896123265E-5	5.079278006027833E-4
19	0.0	0.0	0.0	5.2909145896123265E-5	5.079278006027833E-4
20	0.0	0.0	0.0	5.2909145896123265E-5	6.349097507534791E-4
21	0.0	0.0	0.0	5.2909145896123265E-5	6.454915799327038E-4
22	0.0	0.0	0.0	6.349097507534791E-5	6.772370674703777E-4
23	0.0	0.0	0.0	6.349097507534791E-5	6.772370674703777E-4
24	0.0	0.0	0.0	7.407280425457257E-5	6.772370674703777E-4
25	0.0	0.0	0.0	8.465463343379722E-5	6.772370674703777E-4
26	0.0	0.0	0.0	8.465463343379722E-5	7.724735300833997E-4
27	0.0	0.0	0.0	1.3756377932992047E-4	7.724735300833997E-4
28	0.0	0.0	0.0	5.396732881404573E-4	7.724735300833997E-4
29	0.0	0.0	0.0	0.0013227286474030815	7.724735300833997E-4
30	0.0	0.0	0.0	0.0026666209531646125	7.724735300833997E-4
31	0.0	0.0	0.0	0.007153316525155865	8.042190176210736E-4
32	0.0	0.0	0.0	0.013015649890446323	8.148008468002982E-4
33	0.0	0.0	0.0	0.019322420081264214	8.148008468002982E-4
34	0.0	0.0	0.0	0.026528645752316202	8.465463343379722E-4
35	0.0	0.0	0.0	0.035851237259213124	8.571281635171968E-4
36	0.0	0.0	0.0	0.05131128969006034	8.571281635171968E-4
37	0.0	0.0	0.0	0.0768769889870671	8.571281635171968E-4
38	0.0	0.0	0.0	0.11411444586875866	9.206191385925448E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12160	0.0	27.970499	1
GGTATCA	4715	0.0	26.829369	1
TCAACGC	17915	0.0	18.60341	4
ATCAACG	18300	0.0	18.296078	3
CAACGCA	18590	0.0	17.95159	5
CGTCGTA	3105	0.0	17.925898	10
AACGCAG	18815	0.0	17.760393	6
TATCAAC	19140	0.0	17.745596	2
ACCGTCG	3140	0.0	17.375034	8
CCGTCGT	3180	0.0	16.949928	9
ATACCGT	3260	0.0	16.802767	6
CGCAATA	3050	0.0	16.735195	36
TACCGTC	3355	0.0	16.326979	7
TCTAGCG	3100	0.0	16.323418	28
CTAGCGG	3110	0.0	16.27076	29
TAGATCG	475	0.0	16.209557	5
TATCGAG	320	0.0	15.812475	10
GTCGTAG	3445	0.0	15.709534	11
GGCGTTA	3360	0.0	15.648371	42
CGGTCCA	3475	0.0	15.510768	10
>>END_MODULE