FastQCFastQC Report
Thu 2 Feb 2017
SRR4063920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198529
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48930.22255790121485775No Hit
TATCAACGCAGAGTACTTTTTTTTT32470.14768965976796303No Hit
GTCCTAAAGTGTGTATTTCTCATTT27860.1267210939678303No Hit
GTCCTACAGTGGACATTTCTAAATT25390.11548630925496094No Hit
GTCCTACAGTGTGCATTTCTCATTT24000.10916390004407492No Hit
CTTTAGGACGTGAAATATGGCGAGG22980.10452443429220175No Hit
CTGTAGGACGTGGAATATGGCAAGA22500.10234115629132025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA400.00527624614.2515379
CTTACGC400.00529460214.2440793
CGACAAT551.9585619E-413.81998617
ACGGTAT2600.012.7898419
AACCGCG1501.8189894E-1212.6665917
ACCGTCC853.941661E-612.2951638
AACGGAC701.0895034E-412.21643715
ACGCGCG701.089735E-412.2161613
TCGCGTA1603.6379788E-1211.8762819
CTAGGAC2750.011.7405743
CGAACGA1701.8189894E-1211.73709416
CGATAAC1701.8189894E-1211.73655910
CGTTATT1701.8189894E-1211.729352
TATACCG658.0368796E-411.6903745
CGCGTAA1657.2759576E-1211.51639410
GCGTAAC1657.2759576E-1211.51560711
GCGTTAT1657.2759576E-1211.5135111
GGTCGCG2100.011.3094567
ACGAACG1604.5474735E-1111.28323715
TGGCGAG14950.011.18566218
Produced by FastQC (version 0.11.5)