##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063920_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2198529
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.269655756189707	32.0	32.0	32.0	32.0	32.0
2	30.895772582485836	32.0	32.0	32.0	32.0	32.0
3	30.9072447986813	32.0	32.0	32.0	32.0	32.0
4	30.94044881827804	32.0	32.0	32.0	32.0	32.0
5	30.858186542001494	32.0	32.0	32.0	32.0	32.0
6	34.516373902732234	36.0	36.0	36.0	32.0	36.0
7	34.4501246060434	36.0	36.0	36.0	32.0	36.0
8	34.423399009064696	36.0	36.0	36.0	32.0	36.0
9	34.543191379326814	36.0	36.0	36.0	32.0	36.0
10	34.26049235648017	36.0	36.0	36.0	32.0	36.0
11	34.51565796948778	36.0	36.0	36.0	32.0	36.0
12	34.344600867216215	36.0	36.0	36.0	32.0	36.0
13	34.41897696141375	36.0	36.0	36.0	32.0	36.0
14	34.33415160773408	36.0	36.0	36.0	32.0	36.0
15	34.276655891280036	36.0	36.0	36.0	32.0	36.0
16	34.26854410380759	36.0	36.0	36.0	32.0	36.0
17	34.20591722920189	36.0	36.0	36.0	32.0	36.0
18	34.20017793715707	36.0	36.0	36.0	32.0	36.0
19	34.20342556318338	36.0	36.0	36.0	32.0	36.0
20	34.1854690113253	36.0	36.0	36.0	32.0	36.0
21	34.162393127404734	36.0	36.0	36.0	32.0	36.0
22	34.139213082929544	36.0	36.0	36.0	32.0	36.0
23	34.09869417233068	36.0	36.0	36.0	32.0	36.0
24	34.08214265083608	36.0	36.0	36.0	32.0	36.0
25	33.69777155543547	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	22.0
5	25.0
6	73.0
7	29.0
8	111.0
9	132.0
10	192.0
11	63.0
12	134.0
13	102.0
14	207.0
15	302.0
16	576.0
17	749.0
18	1021.0
19	1293.0
20	1973.0
21	2747.0
22	4580.0
23	6993.0
24	10624.0
25	15811.0
26	22657.0
27	30734.0
28	42277.0
29	57538.0
30	76516.0
31	104539.0
32	148867.0
33	211918.0
34	459811.0
35	995907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72254616950226	17.44665031939963	11.598490615488709	26.2323128956094
2	15.945866404538183	19.78436262886	38.70846833602239	25.561302630579423
3	19.069664411641227	23.970512582004886	28.57461256485681	28.385210441497076
4	12.490766179654663	15.76254071068576	36.18784228816798	35.5588508214916
5	14.313390925090532	36.78891457990936	33.895786243027146	15.001908251972958
6	33.52598605439949	35.842231296081984	17.277923954261922	13.353858695256605
7	29.82682895537788	30.420807822177576	21.052765668603833	18.699597553840707
8	27.3560168331279	34.06920255581809	19.510203115921357	19.064577495132653
9	27.43407089472094	14.230957555635646	18.778236478344603	39.55673507129882
10	15.883519341157754	26.78357936912833	31.91544748599309	25.417453803720825
11	36.62644011890481	20.789227937897177	23.589598195675958	18.99473374752206
12	25.264508905713445	23.24283116806277	29.568470565746622	21.924189360477158
13	29.797908310277172	19.886890915636517	25.325156758395227	24.990044015691087
14	23.21225623951736	20.356199236715923	25.39587680949986	31.035667714266857
15	24.44875889115079	28.77881588462769	22.19209391294375	24.580331311277774
16	24.81020885218683	25.713969720802204	25.281910400372848	24.19391102663812
17	23.35746823391056	26.403754176275342	25.92433301338547	24.314444576428627
18	23.86788566538531	25.169364805167316	27.73991355942619	23.222835970021187
19	25.524913984157337	24.63364465477367	25.526142944470564	24.31529841659843
20	25.738963793778392	24.721648170570724	25.525431752592336	24.013956283058548
21	26.139056964569146	24.164935839925498	24.696359960981862	24.999647234523493
22	25.698557208019768	25.199904775820343	25.03257968766287	24.06895832849702
23	23.840439188266462	25.105791203488742	25.963546645544845	25.090222962699954
24	24.80974630564855	24.96696534034917	25.959666535423214	24.263621818579065
25	24.438540183995045	25.108345505749675	26.002648268679557	24.450466041575723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	133.0
1	133.0
2	404.5
3	676.0
4	676.0
5	676.0
6	1895.5
7	3115.0
8	3115.0
9	3115.0
10	3263.0
11	3411.0
12	3411.0
13	3411.0
14	4316.5
15	5222.0
16	5222.0
17	5222.0
18	9432.0
19	13642.0
20	13642.0
21	13642.0
22	23720.5
23	33799.0
24	33799.0
25	33799.0
26	52281.0
27	70763.0
28	70763.0
29	70763.0
30	93476.5
31	116190.0
32	116190.0
33	116190.0
34	135636.0
35	155082.0
36	155082.0
37	155082.0
38	173384.0
39	191686.0
40	191686.0
41	191686.0
42	214652.5
43	237619.0
44	237619.0
45	237619.0
46	260364.0
47	283109.0
48	283109.0
49	283109.0
50	286993.5
51	290878.0
52	290878.0
53	290878.0
54	267763.0
55	244648.0
56	244648.0
57	244648.0
58	223611.0
59	202574.0
60	202574.0
61	202574.0
62	176608.0
63	150642.0
64	150642.0
65	150642.0
66	122786.0
67	94930.0
68	94930.0
69	94930.0
70	71803.5
71	48677.0
72	48677.0
73	48677.0
74	36830.5
75	24984.0
76	24984.0
77	24984.0
78	20124.5
79	15265.0
80	15265.0
81	15265.0
82	10434.5
83	5604.0
84	5604.0
85	5604.0
86	4308.5
87	3013.0
88	3013.0
89	3013.0
90	2139.5
91	1266.0
92	1266.0
93	1266.0
94	883.0
95	500.0
96	500.0
97	500.0
98	800.5
99	1101.0
100	1101.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036933786181578686
2	0.005276255168796954
3	0.0014555186672543323
4	0.0040481612933011115
5	0.008278262420009015
6	0.012417393630013523
7	0.021423415383649703
8	0.03274917001322248
9	0.04625820264367675
10	0.055491649189071415
11	0.051898337479287286
12	0.06113178402468196
13	0.061768573441605724
14	0.06568027985985174
15	0.057311047523139334
16	0.06263278765028799
17	0.058948506023800466
18	0.07477727153019133
19	0.07050168544513173
20	0.07591439548898377
21	0.07286690327942001
22	0.0731398130295302
23	0.0795531921571196
24	0.07250302361260644
25	0.07377660244645397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2198529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.3942826213631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85328876270337	37.467187829048356
2	13.662984968379066	13.497466819591025
3	4.428438299172533	6.562185987617898
4	1.9650552630715543	3.882499801230134
5	0.9881635608299567	2.4404815099883703
6	0.6275964574703784	1.8599806075474894
7	0.4355296640631271	1.5058872721704997
8	0.29909292107286417	1.181878421881768
9	0.23288699845220104	1.0352957598350072
>10	1.3184192330617481	11.844286714588652
>50	0.09394824445738452	3.2233146595428614
>100	0.0793566500782086	8.32311790552601
>500	0.010805820187646174	3.6562495322713473
>1k	0.00443315700005996	3.520167179160616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4893	0.22255790121485775	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3247	0.14768965976796303	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2786	0.1267210939678303	No Hit
GTCCTACAGTGGACATTTCTAAATT	2539	0.11548630925496094	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2400	0.10916390004407492	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2298	0.10452443429220175	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2250	0.10234115629132025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.5484958351697886E-5
2	0.0	0.0	0.0	0.0	4.5484958351697886E-5
3	0.0	0.0	0.0	0.0	4.5484958351697886E-5
4	0.0	0.0	0.0	0.0	4.5484958351697886E-5
5	0.0	4.5484958351697886E-5	0.0	0.0	4.5484958351697886E-5
6	0.0	4.5484958351697886E-5	0.0	0.0	4.5484958351697886E-5
7	0.0	4.5484958351697886E-5	0.0	0.0	4.5484958351697886E-5
8	0.0	4.5484958351697886E-5	0.0	0.0	4.5484958351697886E-5
9	0.0	4.5484958351697886E-5	0.0	4.5484958351697886E-5	4.5484958351697886E-5
10	0.0	1.3645487505509364E-4	0.0	4.5484958351697886E-5	4.5484958351697886E-5
11	0.0	1.3645487505509364E-4	0.0	4.5484958351697886E-5	4.5484958351697886E-5
12	0.0	1.3645487505509364E-4	0.0	4.5484958351697886E-5	4.5484958351697883E-4
13	0.0	1.3645487505509364E-4	0.0	4.5484958351697886E-5	5.003345418686767E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	40	0.005276246	14.251537	9
CTTACGC	40	0.005294602	14.244079	3
CGACAAT	55	1.9585619E-4	13.819986	17
ACGGTAT	260	0.0	12.789841	9
AACCGCG	150	1.8189894E-12	12.666591	7
ACCGTCC	85	3.941661E-6	12.295163	8
AACGGAC	70	1.0895034E-4	12.216437	15
ACGCGCG	70	1.089735E-4	12.21616	13
TCGCGTA	160	3.6379788E-12	11.876281	9
CTAGGAC	275	0.0	11.740574	3
CGAACGA	170	1.8189894E-12	11.737094	16
CGATAAC	170	1.8189894E-12	11.736559	10
CGTTATT	170	1.8189894E-12	11.72935	2
TATACCG	65	8.0368796E-4	11.690374	5
CGCGTAA	165	7.2759576E-12	11.516394	10
GCGTAAC	165	7.2759576E-12	11.515607	11
GCGTTAT	165	7.2759576E-12	11.513511	1
GGTCGCG	210	0.0	11.309456	7
ACGAACG	160	4.5474735E-11	11.283237	15
TGGCGAG	1495	0.0	11.185662	18
>>END_MODULE