FastQCFastQC Report
Thu 2 Feb 2017
SRR4063920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198529
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37750.021.0965581
TATACCG1201.43318175E-820.166545
TGTAGGA22650.019.424162
GTAGGAC22700.019.3826983
TAGGACG17350.019.2736554
GATATAC9150.019.2348541
GTCCTAA12000.018.6998841
ATACACT10000.018.4798854
GTCCTAC20050.018.2143521
TAGGACC20600.018.1552284
CTGTAGG22800.018.1402381
TACACCG1254.8572656E-717.5998925
TTAGGAC11500.017.5994913
AGGACGT19300.017.212335
GGACGTG19050.017.2072436
GGTATCA20600.016.9804781
ATTTCGT10150.016.90591442
AACGCAG47100.016.7217666
GATTTCG9900.016.66656341
CGAATGT5950.016.64082133
Produced by FastQC (version 0.11.5)