##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063920_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2198529
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.360875385314454	32.0	32.0	32.0	32.0	32.0
2	31.43337067648414	32.0	32.0	32.0	32.0	32.0
3	31.492952787977778	32.0	32.0	32.0	32.0	32.0
4	31.571359759184435	32.0	32.0	32.0	32.0	32.0
5	31.52884587831227	32.0	32.0	32.0	32.0	32.0
6	35.11989107262174	36.0	36.0	36.0	36.0	36.0
7	35.13797134356654	36.0	36.0	36.0	36.0	36.0
8	35.084162182986894	36.0	36.0	36.0	36.0	36.0
9	35.189972477051704	36.0	36.0	36.0	36.0	36.0
10	35.048912704813084	36.0	36.0	36.0	36.0	36.0
11	35.190734350104094	36.0	36.0	36.0	36.0	36.0
12	35.091736793101205	36.0	36.0	36.0	36.0	36.0
13	35.1363993834059	36.0	36.0	36.0	36.0	36.0
14	35.08673708647919	36.0	36.0	36.0	36.0	36.0
15	35.057200973923926	36.0	36.0	36.0	36.0	36.0
16	35.072022702452415	36.0	36.0	36.0	36.0	36.0
17	35.046673935163014	36.0	36.0	36.0	36.0	36.0
18	35.03621330444129	36.0	36.0	36.0	36.0	36.0
19	35.02800235975964	36.0	36.0	36.0	36.0	36.0
20	35.02383320847712	36.0	36.0	36.0	36.0	36.0
21	35.01992423115638	36.0	36.0	36.0	36.0	36.0
22	34.99625203943182	36.0	36.0	36.0	36.0	36.0
23	34.95204520840981	36.0	36.0	36.0	36.0	36.0
24	34.92809373904097	36.0	36.0	36.0	36.0	36.0
25	34.90016188096677	36.0	36.0	36.0	32.0	36.0
26	34.84846140305632	36.0	36.0	36.0	32.0	36.0
27	34.8311543764035	36.0	36.0	36.0	32.0	36.0
28	34.80479766243702	36.0	36.0	36.0	32.0	36.0
29	34.77917052720251	36.0	36.0	36.0	32.0	36.0
30	34.75862269726713	36.0	36.0	36.0	32.0	36.0
31	34.74938879587215	36.0	36.0	36.0	32.0	36.0
32	34.71197923702621	36.0	36.0	36.0	32.0	36.0
33	34.698877294773006	36.0	36.0	36.0	32.0	36.0
34	34.684339392384636	36.0	36.0	36.0	32.0	36.0
35	34.648685098081486	36.0	36.0	36.0	32.0	36.0
36	34.62359650475386	36.0	36.0	36.0	32.0	36.0
37	34.60201252746723	36.0	36.0	36.0	32.0	36.0
38	34.561827476462675	36.0	36.0	36.0	32.0	36.0
39	34.543157720457636	36.0	36.0	36.0	32.0	36.0
40	34.53564860868335	36.0	36.0	36.0	32.0	36.0
41	34.51510032389839	36.0	36.0	36.0	32.0	36.0
42	34.462470133439226	36.0	36.0	36.0	32.0	36.0
43	34.448701836546164	36.0	36.0	36.0	32.0	36.0
44	34.38477363728202	36.0	36.0	36.0	32.0	36.0
45	34.35674489624654	36.0	36.0	36.0	32.0	36.0
46	34.3145530488795	36.0	36.0	36.0	32.0	36.0
47	34.28574696990579	36.0	36.0	36.0	32.0	36.0
48	34.24206958379899	36.0	36.0	36.0	32.0	36.0
49	34.22023180044475	36.0	36.0	36.0	32.0	36.0
50	33.634524265997854	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	1.0
19	7.0
20	17.0
21	58.0
22	195.0
23	590.0
24	1510.0
25	3546.0
26	7441.0
27	13821.0
28	24000.0
29	36697.0
30	54371.0
31	78155.0
32	113829.0
33	183214.0
34	380735.0
35	1300339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13572078081878	18.225276217396704	12.146287298844275	26.492715702940238
2	15.333413083989722	20.489202049197665	38.48590801353093	25.691476853281685
3	18.236888236733588	24.596251481677236	28.970053517772392	28.196806763816785
4	12.203614325760542	16.442721474222083	36.37923356935478	34.974430630662596
5	13.939183881586278	37.231576203907245	33.719455144780895	15.109784769725575
6	33.652198633174514	36.115622974494265	17.05147769527297	13.180700697058256
7	29.89515252712384	30.50483346349624	20.992782895092944	18.607231114286975
8	27.36579776750727	34.31317030614561	19.367813660861422	18.953218265485695
9	27.601753560840177	14.088618684210767	18.53765616343522	39.77197159151385
10	15.86109431935152	26.966398438207158	31.923234897991765	25.24927234444955
11	36.82524708588978	20.737111711614183	23.606623889540586	18.83101731295545
12	25.315188448988096	23.277459534251584	29.48561900109437	21.92173301566595
13	29.873828929695073	19.972272293717193	25.244584659792054	24.909314116795684
14	23.271000661352197	20.406354075412345	25.378230687287758	30.944414575947704
15	24.565600272193507	28.801975586280427	22.170488474533716	24.461935666992353
16	24.87708760041428	25.83268630626918	25.156366769053868	24.133859324262673
17	23.452726800510707	26.50040094990787	25.85665233435629	24.190219915225132
18	24.12325189377227	25.075209618097976	27.66340295889693	23.13813552923282
19	25.717207149510944	24.578490699231487	25.538269241965533	24.166032909292035
20	25.959451831481257	24.77742991765828	25.41877016412397	23.844348086736492
21	26.33447182184088	24.222559720613194	24.636836721280456	24.806131736265478
22	25.83297817129378	25.123002810518138	25.11981886053708	23.92420015765101
23	24.03939748448499	25.036888519346682	25.90665703592787	25.017056960240453
24	25.01826233537744	24.961041867370415	25.844866123208675	24.175829674043467
25	24.681555606095067	24.97634750966568	26.049078917443712	24.293017966795542
26	23.810600340244356	26.03169549039765	26.467872381073683	23.689831788284312
27	24.470141710994	25.47017901054304	25.62883855572507	24.430840722737894
28	23.978020928333763	25.42535553377258	25.763729528236873	24.83289400965678
29	24.29974473318887	25.034068931136183	25.491065661610456	25.175120674064495
30	23.880227013558134	25.363720678893714	26.123707560614047	24.632344746934102
31	25.204445071165715	24.76507807986754	25.195120065138575	24.835356783828168
32	24.4206753348359	25.589627308144546	25.175611165062893	24.81408619195666
33	23.93349277987371	25.334805894002926	26.031209431423584	24.700491894699784
34	24.74252501516967	25.323930413297273	26.05025504146983	23.883289530063234
35	24.884636255669253	25.393851482000528	25.910662862221688	23.81084940010853
36	23.898281646882438	25.654435257898527	25.872504530600658	24.574778564618374
37	24.81070754395142	25.41577020173304	25.079169414814984	24.694352839500556
38	24.39218768763851	25.773843964817665	25.351304781347682	24.482663566196138
39	25.277503467229735	24.870487768377682	25.13276453632369	24.71924422806889
40	25.767233936870987	25.153947132612416	25.572690611067088	23.506128319449513
41	24.699438290408725	25.029349997066134	26.264255956536985	24.006955755988148
42	25.206095064816918	25.99667955424153	25.536820559472368	23.260404821469184
43	24.201888376721968	25.33543294479605	26.03409048524859	24.42858819323339
44	23.94591061487581	25.793435460123206	25.3253909808875	24.935262944113486
45	24.040518443214822	25.9816557160434	25.436239626473444	24.54158621426833
46	24.303949354813327	25.04795290994678	25.432031042828534	25.216066692411367
47	25.07118502831026	25.061678529321863	25.588720652664364	24.278415789703505
48	24.87577871417551	25.60518645473997	24.841482540791525	24.677552290293
49	24.736117693828298	25.540932573670084	25.03481890982926	24.688130822672363
50	24.322051282984315	26.4811983768985	24.711997733006992	24.484752607110188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	29.0
2	39.0
3	111.5
4	184.0
5	222.5
6	261.0
7	254.5
8	248.0
9	372.0
10	496.0
11	718.0
12	940.0
13	1801.5
14	2663.0
15	3665.5
16	4668.0
17	5022.5
18	5377.0
19	5472.0
20	5567.0
21	6170.0
22	6773.0
23	7949.5
24	9126.0
25	11147.5
26	13169.0
27	18081.0
28	22993.0
29	28298.0
30	33603.0
31	40123.0
32	46643.0
33	56388.0
34	66133.0
35	77073.0
36	88013.0
37	95228.5
38	102444.0
39	104907.0
40	107370.0
41	110886.0
42	114402.0
43	115164.5
44	115927.0
45	126019.5
46	136112.0
47	143696.5
48	151281.0
49	155780.5
50	160280.0
51	157457.5
52	154635.0
53	153126.0
54	151617.0
55	150968.5
56	150320.0
57	145831.0
58	141342.0
59	129024.0
60	116706.0
61	102525.5
62	88345.0
63	76330.0
64	64315.0
65	54440.5
66	44566.0
67	38029.0
68	31492.0
69	27801.0
70	24110.0
71	18534.0
72	12958.0
73	10989.5
74	9021.0
75	7005.5
76	4990.0
77	4335.5
78	3681.0
79	3020.5
80	2360.0
81	1937.5
82	1515.0
83	1223.0
84	931.0
85	668.5
86	406.0
87	299.0
88	192.0
89	147.5
90	103.0
91	82.0
92	61.0
93	49.0
94	37.0
95	45.0
96	53.0
97	39.0
98	25.0
99	21.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00773244291978864
2	4.548495835169789E-4
3	3.183947084618852E-4
4	0.0
5	0.0
6	1.8193983340679154E-4
7	9.096991670339577E-5
8	0.0
9	0.0014100337089026345
10	0.0013645487505509367
11	9.096991670339577E-5
12	1.3645487505509364E-4
13	2.729097501101873E-4
14	1.3645487505509364E-4
15	0.004230101126707904
16	5.458195002203746E-4
17	0.0
18	3.183947084618852E-4
19	4.5484958351697886E-5
20	3.638796668135831E-4
21	0.0
22	9.096991670339577E-5
23	5.913044585720725E-4
24	6.822743752754683E-4
25	0.0013190637921992386
26	0.004957860460335069
27	0.005048830377038465
28	0.0033658869180256436
29	0.002046823125826405
30	0.003183947084618852
31	0.006322409210886007
32	0.0029110373345086647
33	0.004139131210004508
34	0.0028655523761569666
35	0.0021832780008814983
36	0.006595318960996193
37	0.0035933117097841326
38	0.006959198627809776
39	0.004002676334949414
40	0.0021377930425298007
41	0.002820067417805269
42	0.0013190637921992386
43	0.001228093875495843
44	9.096991670339578E-4
45	0.0010916390004407492
46	8.187292503305619E-4
47	0.0015010036256060302
48	0.0015010036256060302
49	5.003345418686767E-4
50	0.0010006690837373535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2198529.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.94129346047354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12543411135525	41.60245674667029
2	13.345014460134488	14.396946824567545
3	4.092891505801634	6.623275854489761
4	1.7537591176954692	3.7840014090636984
5	0.9411424760607427	2.5383221244654606
6	0.5724122613790291	1.8525994662851695
7	0.3812425696653483	1.4395302130960508
8	0.28824860800359015	1.2438802203115726
9	0.20700981442449956	1.004973943416309
>10	1.1458924884666486	11.051900316898168
>50	0.0761978635639883	2.8381295833838074
>100	0.060250959312406274	6.84583036035375
>500	0.007877823102700646	2.9064979948979968
>1k	0.0026259410342335168	1.871654942100466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3645487505509364E-4
2	0.0	0.0	0.0	0.0	1.3645487505509364E-4
3	0.0	0.0	0.0	0.0	1.3645487505509364E-4
4	0.0	0.0	0.0	0.0	1.3645487505509364E-4
5	0.0	4.5484958351697886E-5	0.0	0.0	1.8193983340679154E-4
6	0.0	4.5484958351697886E-5	0.0	0.0	3.183947084618852E-4
7	0.0	4.5484958351697886E-5	0.0	0.0	3.183947084618852E-4
8	0.0	4.5484958351697886E-5	0.0	0.0	3.183947084618852E-4
9	0.0	4.5484958351697886E-5	0.0	0.0	3.183947084618852E-4
10	0.0	4.5484958351697886E-5	0.0	0.0	3.183947084618852E-4
11	0.0	4.5484958351697886E-5	0.0	0.0	3.183947084618852E-4
12	0.0	4.5484958351697886E-5	0.0	0.0	5.913044585720725E-4
13	0.0	4.5484958351697886E-5	0.0	0.0	6.822743752754683E-4
14	0.0	4.5484958351697886E-5	0.0	0.0	6.822743752754683E-4
15	0.0	4.5484958351697886E-5	0.0	0.0	7.73244291978864E-4
16	0.0	9.096991670339577E-5	0.0	0.0	9.096991670339577E-4
17	0.0	9.096991670339577E-5	0.0	0.0	9.551841253856556E-4
18	0.0	9.096991670339577E-5	0.0	4.5484958351697886E-5	9.551841253856556E-4
19	0.0	9.096991670339577E-5	0.0	4.5484958351697886E-5	9.551841253856556E-4
20	0.0	9.096991670339577E-5	0.0	1.3645487505509364E-4	0.0010461540420890513
21	0.0	9.096991670339577E-5	0.0	1.3645487505509364E-4	0.0011371239587924472
22	0.0	9.096991670339577E-5	0.0	1.3645487505509364E-4	0.001228093875495843
23	0.0	9.096991670339577E-5	0.0	2.2742479175848942E-4	0.001228093875495843
24	0.0	9.096991670339577E-5	0.0	3.183947084618852E-4	0.0013190637921992386
25	0.0	9.096991670339577E-5	0.0	4.09364625165281E-4	0.0013190637921992386
26	0.0	9.096991670339577E-5	0.0	5.458195002203746E-4	0.0013645487505509367
27	0.0	9.096991670339577E-5	0.0	7.73244291978864E-4	0.0015010036256060302
28	0.0	9.096991670339577E-5	0.0	0.0018648832924196134	0.0015010036256060302
29	0.0	9.096991670339577E-5	0.0	0.0038207365015426225	0.0015010036256060302
30	0.0	9.096991670339577E-5	0.0	0.0076869579614369426	0.0015010036256060302
31	0.0	9.096991670339577E-5	0.0	0.01955853209123009	0.0015010036256060302
32	0.0	9.096991670339577E-5	0.0	0.03202141067959531	0.0015010036256060302
33	0.0	1.3645487505509364E-4	0.0	0.04471171405971902	0.0015010036256060302
34	0.0	1.3645487505509364E-4	0.0	0.061177268983033654	0.0015919735423094259
35	4.5484958351697886E-5	1.3645487505509364E-4	0.0	0.07882543282349244	0.0015919735423094259
36	4.5484958351697886E-5	1.3645487505509364E-4	0.0	0.10520670866747721	0.0015919735423094259
37	4.5484958351697886E-5	1.3645487505509364E-4	0.0	0.14891775364345888	0.0015919735423094259
38	4.5484958351697886E-5	1.3645487505509364E-4	0.0	0.20918532345945856	0.0017284284173645196
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3775	0.0	21.096558	1
TATACCG	120	1.43318175E-8	20.16654	5
TGTAGGA	2265	0.0	19.42416	2
GTAGGAC	2270	0.0	19.382698	3
TAGGACG	1735	0.0	19.273655	4
GATATAC	915	0.0	19.234854	1
GTCCTAA	1200	0.0	18.699884	1
ATACACT	1000	0.0	18.479885	4
GTCCTAC	2005	0.0	18.214352	1
TAGGACC	2060	0.0	18.155228	4
CTGTAGG	2280	0.0	18.140238	1
TACACCG	125	4.8572656E-7	17.599892	5
TTAGGAC	1150	0.0	17.599491	3
AGGACGT	1930	0.0	17.21233	5
GGACGTG	1905	0.0	17.207243	6
GGTATCA	2060	0.0	16.980478	1
ATTTCGT	1015	0.0	16.905914	42
AACGCAG	4710	0.0	16.721766	6
GATTTCG	990	0.0	16.666563	41
CGAATGT	595	0.0	16.640821	33
>>END_MODULE