FastQCFastQC Report
Thu 2 Feb 2017
SRR4063919_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063919_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002616
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT246111.2289425431535552No Hit
GTCCTACAGTGGACATTTCTAAATT234161.1692705940629657No Hit
CTGTAGGACGTGGAATATGGCAAGA227571.1363636363636365No Hit
CTTTAGGACGTGAAATATGGCGAGG202861.0129750286625094No Hit
GTCCTACAGTGTGCATTTCTCATTT163480.8163322374334372No Hit
CTGTAGGACCTGGAATATGGCGAGA116410.5812896731075753No Hit
CTGAAGGACCTGGAATATGGCGAGA106700.5328030935536319No Hit
ATTTAGAAATGTCCACTGTAGGACG97500.4868631829566926No Hit
GTCCTTCAGTGTGCATTTCTCATTT92470.46174603618467047No Hit
GATATACACTGTTCTACAAATCCCG81870.40881526962732745No Hit
TTGTAGAACAGTGTATATCAATGAG77230.38564557558713203No Hit
TTTCTAAATTTTCCACCTTTTTCAG73990.36946673750734044No Hit
GAATATGGCAAGAAAACTGAAAATC65550.3273218630031918No Hit
GTGTATATCAATGAGTTACAATGAA63210.3156371466122312No Hit
GTATCAACGCAGAGTACTTTTTTTT59990.29955817790330247No Hit
CTGTAGGACATGGAATATGGCAAGA57790.28857254710838226No Hit
GGAATATGGCGAGAAAACTGAAAAT55610.27768678568432487No Hit
GTGTATATCAATGAGTTACAATGAG53960.26944756258813474No Hit
ATTCCAGGTCCTTCAGTGTGCATTT50890.25411761416067785No Hit
GTTCTACAGTGTGGTTTTTATCATT48350.2414342040610881No Hit
TTCCAGGTCCTTCAGTGTGCATTTC47740.2383881882497693No Hit
GATATACACTGTTCTACAATGCCGG47600.23768910265372892No Hit
CCATATTCCAGGTCCTTCAGTGTGC46800.23369432781921248No Hit
GCCATATTCCACGTCCTACAGTGGA45010.2247560191269819No Hit
GACCTGGAATATGGCGAGAAAACTG43910.2192632037295218No Hit
GAAATATGGCGAGGAAAACTGAAAA43390.2166666000870861No Hit
ACCTGGAATATGGCGAGAAAACTGA43330.21636699197449738No Hit
TATCAACGCAGAGTACTTTTTTTTT43200.21571784106388844No Hit
ACAGTGGACATTTCTAAATTTTCCA42750.21347078021947294No Hit
GTCCACTGTAGGACGTGGAATATGG42020.20982554818297663No Hit
CCATATTTCACGTCCTAAAGTGTGT40220.20083730480531464No Hit
ATACACACTTTAGGACGTGAAATAT38350.19149951862963244No Hit
GTTCTACAAATCCCGTTTCCAACGA37170.18560722574872068No Hit
CTGTAGAACATATTAGATGAGTGAG36010.17981480223867183No Hit
CCACTGTAGGACGTGGAATATGGCA35580.17766761076511922No Hit
CCTAAAGTGTGTATTTCTCATTTTC35040.1749711377518206No Hit
CACTTTAGGACGTGAAATATGGCGA34590.1727240769074051No Hit
GAAATACACACTTTAGGACGTGAAA32730.16343622541715438No Hit
GTGTATTTCTCATTTTCCGTGATTT32720.1633862907317229No Hit
CTACAGTGGACATTTCTAAATTTTC31230.155946022602436No Hit
ATGTTCTACAGTGTGGTTTTTATCA31030.1549473288938069No Hit
AGTGTGTATTTCTCATTTTCCGTGA30610.15285007210568577No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29940.14950444818177824No Hit
TCCTAAAGTGTGTATTTCTCATTTT28680.14321267781741484No Hit
GTCCTACAGTGGACATTTCTAAATA28390.1417645719399026No Hit
ATTCCACGTCCTACAGTGGACATTT27840.13901816424117255No Hit
GTAGGACGTGGAATATGGCAAGAAA27090.13527306283381338No Hit
GTAGAACATATTAGATGAGTGAGTT26700.1333256101019866No Hit
ATATTTCACGTCCTAAAGTGTGTAT24270.12119148154214289No Hit
GTAGAACAGTGTATATCAATGAGTT24270.12119148154214289No Hit
TTTCTAAATATTCCACCTTTTTCAG23570.11769605356194099No Hit
GTATCAACGCAGAGTACATGGGAAA23050.1150994499195053No Hit
GTGCATTTCTCATTTTTCACGTTTT22010.10990624263463389No Hit
GGTCCTTCAGTGTGCATTTCTCATT21950.10960663452204515No Hit
GTACATGGGGTGGTATCAACGCAAA21890.10930702640945644No Hit
CATCTAATATGTTCTACAGTGTGGT21560.1076591817902184No Hit
GGTATCAACGCAGAGTACTTTTTTT21550.10760924710478693No Hit
GTATTTCTCATTTTCCGTGATTTTC21480.10725970430676675No Hit
TTTTTCAAGTCGTCAAGTGGATGTT21160.10566179437296018No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21060.1051624475186456No Hit
TATTAGATGAGTGAGTTACACTGAA20900.10436349255174232No Hit
ACGTGGAATATGGCAAGAAAACTGA20650.10311512541595594No Hit
CACTGAAGGACCTGGAATATGGCGA20470.10221630107818973No Hit
TTGATATACACTGTTCTACAAATCC20430.10201656233646389No Hit
GTTCTACAATGCCGGTTTCCAACGT20300.101367411425855No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20190.10081812988610897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGGG807.697963E-915.44066818
CCAACGA8400.015.15825219
TAGGACC38800.014.7601974
CTAATAG851.7264028E-814.5236823
TCCAACG11500.014.37686318
TGGCGAC801.2858072E-714.25292518
CCGGTGT602.5652302E-514.252213513
GCGTATT400.0052761214.251511
TGCGTAT400.005277869814.25078810
AAATGTC26400.013.8891837
GTCCTAT5550.013.8625141
GGCGACA551.9567706E-413.82136319
CCACCTT20000.013.77713913
TAGAAAT27550.013.7204384
AGTGCGC707.26277E-613.5718398
GTGTTAA707.277782E-613.5691271
ATAGGAC5950.013.5660753
GAAATGT27000.013.5446766
AATGTCC28250.013.4517358
CTAGGAC5800.013.2620093
Produced by FastQC (version 0.11.5)