##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063919_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2002616
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.380410922513352	32.0	32.0	32.0	32.0	32.0
2	31.151646646186787	32.0	32.0	32.0	32.0	32.0
3	31.15530186516037	32.0	32.0	32.0	32.0	32.0
4	31.162179868731698	32.0	32.0	32.0	32.0	32.0
5	31.211356545638306	32.0	32.0	32.0	32.0	32.0
6	34.87810943286182	36.0	36.0	36.0	36.0	36.0
7	34.80104073871376	36.0	36.0	36.0	36.0	36.0
8	34.78771466921267	36.0	36.0	36.0	36.0	36.0
9	34.801628469961294	36.0	36.0	36.0	36.0	36.0
10	34.63529353605484	36.0	36.0	36.0	32.0	36.0
11	34.76985602831496	36.0	36.0	36.0	36.0	36.0
12	34.66520940609683	36.0	36.0	36.0	32.0	36.0
13	34.691861045752155	36.0	36.0	36.0	32.0	36.0
14	34.67797071430569	36.0	36.0	36.0	32.0	36.0
15	34.657784118373165	36.0	36.0	36.0	32.0	36.0
16	34.65135402893016	36.0	36.0	36.0	32.0	36.0
17	34.64215506117998	36.0	36.0	36.0	32.0	36.0
18	34.65164315075881	36.0	36.0	36.0	32.0	36.0
19	34.51682349486871	36.0	36.0	36.0	32.0	36.0
20	34.48485580860235	36.0	36.0	36.0	32.0	36.0
21	34.44960291938145	36.0	36.0	36.0	32.0	36.0
22	34.390855261318194	36.0	36.0	36.0	32.0	36.0
23	34.43537502946146	36.0	36.0	36.0	32.0	36.0
24	34.36596781409916	36.0	36.0	36.0	32.0	36.0
25	34.0867545250812	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	21.0
5	27.0
6	75.0
7	19.0
8	98.0
9	136.0
10	196.0
11	47.0
12	137.0
13	134.0
14	182.0
15	316.0
16	541.0
17	650.0
18	835.0
19	969.0
20	1431.0
21	2022.0
22	3077.0
23	4629.0
24	6877.0
25	10255.0
26	15145.0
27	20757.0
28	29077.0
29	40350.0
30	54531.0
31	76334.0
32	110053.0
33	163098.0
34	394264.0
35	1066324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.527777985925546	20.074553535523084	12.52083767321433	25.876830805337043
2	9.62903777537522	20.256280290337802	53.02644950424346	17.088232430043522
3	19.878376772340452	26.31113494705325	30.504729403798503	23.305758876807793
4	9.59126704718531	17.57581659201111	41.652659385876866	31.180256974926717
5	8.684246403974392	42.510483570121934	38.533343920528125	10.271926105375547
6	28.54979666219355	41.25732333233284	19.315218797272983	10.877661208200633
7	25.369717826220512	32.14034018685054	22.86399268780577	19.625949299123185
8	19.64860084230697	48.99162438564092	19.57297204681792	11.786802725234192
9	27.057194829928306	14.137411277095238	21.068676661685647	37.73671723129081
10	14.918516712368191	30.243450558411343	37.24137999952033	17.59665272970013
11	33.71586883640524	19.047500087435733	31.705096753885893	15.53153432227313
12	27.23838590673948	22.479921770731682	36.93934483487458	13.342347487654257
13	32.45995154060211	24.24803736440438	26.093172161779837	17.198838933213676
14	16.56969902302788	28.366321320938663	27.64249946156848	27.421480194464976
15	17.68491541506005	45.20888535351693	21.857675851582997	15.248523379840018
16	15.165473464131718	27.575446095511836	40.276694398402554	16.98238604195389
17	15.775855995227023	33.55694167744734	33.707046585987776	16.96015574133786
18	15.827504231585543	27.334918189346148	43.17646523803075	13.66111234103756
19	24.634758087044386	24.241568639993282	27.705513697415036	23.418159575547296
20	23.699242574168363	30.57824434729244	31.622678396871944	14.099834681667256
21	19.534150844013645	27.078450441121937	26.767910990018162	26.619487724846252
22	21.924779324933947	36.067223749499625	27.587943942561267	14.420052983005158
23	17.072395480892347	34.92657899392581	31.805174204343388	16.195851320838454
24	24.221592317471995	27.452763807041254	33.601414886897004	14.724228988589752
25	19.475702410943324	32.00583108154369	33.65555539452504	14.86291111298795
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	201.0
1	201.0
2	532.5
3	864.0
4	864.0
5	864.0
6	2686.0
7	4508.0
8	4508.0
9	4508.0
10	4589.0
11	4670.0
12	4670.0
13	4670.0
14	5649.5
15	6629.0
16	6629.0
17	6629.0
18	13723.5
19	20818.0
20	20818.0
21	20818.0
22	43215.0
23	65612.0
24	65612.0
25	65612.0
26	117948.0
27	170284.0
28	170284.0
29	170284.0
30	243692.5
31	317101.0
32	317101.0
33	317101.0
34	319656.5
35	322212.0
36	322212.0
37	322212.0
38	308411.0
39	294610.0
40	294610.0
41	294610.0
42	279492.5
43	264375.0
44	264375.0
45	264375.0
46	255164.5
47	245954.0
48	245954.0
49	245954.0
50	204066.5
51	162179.0
52	162179.0
53	162179.0
54	111634.0
55	61089.0
56	61089.0
57	61089.0
58	45706.5
59	30324.0
60	30324.0
61	30324.0
62	23072.0
63	15820.0
64	15820.0
65	15820.0
66	11996.5
67	8173.0
68	8173.0
69	8173.0
70	5806.0
71	3439.0
72	3439.0
73	3439.0
74	2635.5
75	1832.0
76	1832.0
77	1832.0
78	1258.0
79	684.0
80	684.0
81	684.0
82	456.0
83	228.0
84	228.0
85	228.0
86	169.5
87	111.0
88	111.0
89	111.0
90	92.0
91	73.0
92	73.0
93	73.0
94	66.0
95	59.0
96	59.0
97	59.0
98	413.0
99	767.0
100	767.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.041595592964402565
2	0.006042096937206134
3	0.0018975180463953148
4	0.004294382947105187
5	0.009837133029996765
6	0.01413151597710195
7	0.02401858369253017
8	0.036602124421256994
9	0.050883444454653315
10	0.06112005496810172
11	0.05722514950444818
12	0.06586385008409001
13	0.0675616293887595
14	0.07155640422327596
15	0.06266803021647685
16	0.07085731862723557
17	0.06736189064703368
18	0.07954595389230887
19	0.07714908899159899
20	0.08129366788240981
21	0.07899667235256284
22	0.07974569263403469
23	0.08438961837916005
24	0.0802949741737807
25	0.08064451697180089
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2002616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.283733926678014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.45554550147324	20.632014481240983
2	15.110242150122152	8.849686213837044
3	5.49273333424364	4.8254322447055715
4	2.6424775156214717	3.095264338987533
5	1.4567042562941568	2.132886992558873
6	0.9121636032097171	1.6026933752395955
7	0.6115268386124236	1.2535452461664145
8	0.42664578601217185	0.9995025342815075
9	0.34267782757445847	0.903139769273624
>10	2.06740456379559	11.703685301873781
>50	0.240975102170568	4.88980825520444
>100	0.19371451876645626	11.597803892595179
>500	0.023936450342624788	4.801097662348866
>1k	0.020004033500622145	12.062274722765471
>5k	0.0020516957436535537	4.155924504243629
>10k+	0.0011968225171312395	6.495240464677512
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	24611	1.2289425431535552	No Hit
GTCCTACAGTGGACATTTCTAAATT	23416	1.1692705940629657	No Hit
CTGTAGGACGTGGAATATGGCAAGA	22757	1.1363636363636365	No Hit
CTTTAGGACGTGAAATATGGCGAGG	20286	1.0129750286625094	No Hit
GTCCTACAGTGTGCATTTCTCATTT	16348	0.8163322374334372	No Hit
CTGTAGGACCTGGAATATGGCGAGA	11641	0.5812896731075753	No Hit
CTGAAGGACCTGGAATATGGCGAGA	10670	0.5328030935536319	No Hit
ATTTAGAAATGTCCACTGTAGGACG	9750	0.4868631829566926	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	9247	0.46174603618467047	No Hit
GATATACACTGTTCTACAAATCCCG	8187	0.40881526962732745	No Hit
TTGTAGAACAGTGTATATCAATGAG	7723	0.38564557558713203	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	7399	0.36946673750734044	No Hit
GAATATGGCAAGAAAACTGAAAATC	6555	0.3273218630031918	No Hit
GTGTATATCAATGAGTTACAATGAA	6321	0.3156371466122312	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5999	0.29955817790330247	No Hit
CTGTAGGACATGGAATATGGCAAGA	5779	0.28857254710838226	No Hit
GGAATATGGCGAGAAAACTGAAAAT	5561	0.27768678568432487	No Hit
GTGTATATCAATGAGTTACAATGAG	5396	0.26944756258813474	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	5089	0.25411761416067785	No Hit
GTTCTACAGTGTGGTTTTTATCATT	4835	0.2414342040610881	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	4774	0.2383881882497693	No Hit
GATATACACTGTTCTACAATGCCGG	4760	0.23768910265372892	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	4680	0.23369432781921248	No Hit
GCCATATTCCACGTCCTACAGTGGA	4501	0.2247560191269819	No Hit
GACCTGGAATATGGCGAGAAAACTG	4391	0.2192632037295218	No Hit
GAAATATGGCGAGGAAAACTGAAAA	4339	0.2166666000870861	No Hit
ACCTGGAATATGGCGAGAAAACTGA	4333	0.21636699197449738	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4320	0.21571784106388844	No Hit
ACAGTGGACATTTCTAAATTTTCCA	4275	0.21347078021947294	No Hit
GTCCACTGTAGGACGTGGAATATGG	4202	0.20982554818297663	No Hit
CCATATTTCACGTCCTAAAGTGTGT	4022	0.20083730480531464	No Hit
ATACACACTTTAGGACGTGAAATAT	3835	0.19149951862963244	No Hit
GTTCTACAAATCCCGTTTCCAACGA	3717	0.18560722574872068	No Hit
CTGTAGAACATATTAGATGAGTGAG	3601	0.17981480223867183	No Hit
CCACTGTAGGACGTGGAATATGGCA	3558	0.17766761076511922	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	3504	0.1749711377518206	No Hit
CACTTTAGGACGTGAAATATGGCGA	3459	0.1727240769074051	No Hit
GAAATACACACTTTAGGACGTGAAA	3273	0.16343622541715438	No Hit
GTGTATTTCTCATTTTCCGTGATTT	3272	0.1633862907317229	No Hit
CTACAGTGGACATTTCTAAATTTTC	3123	0.155946022602436	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	3103	0.1549473288938069	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	3061	0.15285007210568577	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2994	0.14950444818177824	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	2868	0.14321267781741484	No Hit
GTCCTACAGTGGACATTTCTAAATA	2839	0.1417645719399026	No Hit
ATTCCACGTCCTACAGTGGACATTT	2784	0.13901816424117255	No Hit
GTAGGACGTGGAATATGGCAAGAAA	2709	0.13527306283381338	No Hit
GTAGAACATATTAGATGAGTGAGTT	2670	0.1333256101019866	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	2427	0.12119148154214289	No Hit
GTAGAACAGTGTATATCAATGAGTT	2427	0.12119148154214289	No Hit
TTTCTAAATATTCCACCTTTTTCAG	2357	0.11769605356194099	No Hit
GTATCAACGCAGAGTACATGGGAAA	2305	0.1150994499195053	No Hit
GTGCATTTCTCATTTTTCACGTTTT	2201	0.10990624263463389	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	2195	0.10960663452204515	No Hit
GTACATGGGGTGGTATCAACGCAAA	2189	0.10930702640945644	No Hit
CATCTAATATGTTCTACAGTGTGGT	2156	0.1076591817902184	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2155	0.10760924710478693	No Hit
GTATTTCTCATTTTCCGTGATTTTC	2148	0.10725970430676675	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	2116	0.10566179437296018	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2106	0.1051624475186456	No Hit
TATTAGATGAGTGAGTTACACTGAA	2090	0.10436349255174232	No Hit
ACGTGGAATATGGCAAGAAAACTGA	2065	0.10311512541595594	No Hit
CACTGAAGGACCTGGAATATGGCGA	2047	0.10221630107818973	No Hit
TTGATATACACTGTTCTACAAATCC	2043	0.10201656233646389	No Hit
GTTCTACAATGCCGGTTTCCAACGT	2030	0.101367411425855	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2019	0.10081812988610897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGGG	80	7.697963E-9	15.440668	18
CCAACGA	840	0.0	15.158252	19
TAGGACC	3880	0.0	14.760197	4
CTAATAG	85	1.7264028E-8	14.523682	3
TCCAACG	1150	0.0	14.376863	18
TGGCGAC	80	1.2858072E-7	14.252925	18
CCGGTGT	60	2.5652302E-5	14.2522135	13
GCGTATT	40	0.00527612	14.2515	11
TGCGTAT	40	0.0052778698	14.250788	10
AAATGTC	2640	0.0	13.889183	7
GTCCTAT	555	0.0	13.862514	1
GGCGACA	55	1.9567706E-4	13.821363	19
CCACCTT	2000	0.0	13.777139	13
TAGAAAT	2755	0.0	13.720438	4
AGTGCGC	70	7.26277E-6	13.571839	8
GTGTTAA	70	7.277782E-6	13.569127	1
ATAGGAC	595	0.0	13.566075	3
GAAATGT	2700	0.0	13.544676	6
AATGTCC	2825	0.0	13.451735	8
CTAGGAC	580	0.0	13.262009	3
>>END_MODULE