FastQCFastQC Report
Thu 2 Feb 2017
SRR4063919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002616
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA120640.6024120450450811No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA92070.4597486487674122No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA89130.44506785125056425No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC89040.4446184390816812No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA58760.2934162115952334No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA47110.23524230306758762No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG45740.22840125116347815No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA41390.20667966300079496No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA36960.1845585973546601No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC35770.17861636978831688No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT34520.1723745341093849No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA33010.1648343966092351No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC32460.16208798891050508No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA31310.15634550008588766No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA29680.14820614636056037No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG26970.1346738466086359No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT26120.13042939834696218No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA23800.11884455132686446No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA22720.11345160530026725No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT22340.11155408725387193No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG21510.10740950836306111No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC21360.10666048808158927No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC21060.1051624475186456No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT21040.1050625781477827No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACA20910.10441342723717377No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG20590.1028155173033672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC44000.030.4470124
GACGTGT1750.026.3974087
CCTAGAG6550.025.1889953
GTCCTAG7350.025.1435281
GGACCTG82950.024.7160786
AGGACCT85400.024.4964265
GACGTTA901.5154E-824.4420457
TATAGGA7550.024.183662
GTGCGTA551.5951894E-423.9976439
TACGGCG1301.8189894E-1223.69116416
CTGTAGG140800.023.2974971
TGTAGGA144500.023.1706242
CGTGTAA1750.022.626359
CTAGAGT7800.022.5618884
GTCGAGG4400.022.49835419
GTAAGAC2350.022.4664423
TAGAGTG7850.022.418185
ATAGGAC7750.022.1403143
ACTGTTC34250.022.0299558
GTAGGAC148450.022.0206183
Produced by FastQC (version 0.11.5)