##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063919_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2002616
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35841419423394	32.0	32.0	32.0	32.0	32.0
2	31.54792830977082	32.0	32.0	32.0	32.0	32.0
3	31.590741809712895	32.0	32.0	32.0	32.0	32.0
4	31.64962978424221	32.0	32.0	32.0	32.0	32.0
5	31.64228039724041	32.0	32.0	32.0	32.0	32.0
6	35.25943915358711	36.0	36.0	36.0	36.0	36.0
7	35.28097748145426	36.0	36.0	36.0	36.0	36.0
8	35.22746697319906	36.0	36.0	36.0	36.0	36.0
9	35.31595672859899	36.0	36.0	36.0	36.0	36.0
10	35.22375233194981	36.0	36.0	36.0	36.0	36.0
11	35.30730754173541	36.0	36.0	36.0	36.0	36.0
12	35.26309836733552	36.0	36.0	36.0	36.0	36.0
13	35.278406843848245	36.0	36.0	36.0	36.0	36.0
14	35.24600322777807	36.0	36.0	36.0	36.0	36.0
15	35.2177212206434	36.0	36.0	36.0	36.0	36.0
16	35.24889344737084	36.0	36.0	36.0	36.0	36.0
17	35.259587958949695	36.0	36.0	36.0	36.0	36.0
18	35.25464142901085	36.0	36.0	36.0	36.0	36.0
19	35.18992557734483	36.0	36.0	36.0	36.0	36.0
20	35.17743092035617	36.0	36.0	36.0	36.0	36.0
21	35.1374067719423	36.0	36.0	36.0	36.0	36.0
22	35.09905144071554	36.0	36.0	36.0	36.0	36.0
23	35.11777544971178	36.0	36.0	36.0	36.0	36.0
24	35.094823970246914	36.0	36.0	36.0	36.0	36.0
25	35.050825520219554	36.0	36.0	36.0	36.0	36.0
26	35.020018316042616	36.0	36.0	36.0	36.0	36.0
27	34.941400148605624	36.0	36.0	36.0	36.0	36.0
28	34.86054940138299	36.0	36.0	36.0	36.0	36.0
29	34.83989541679483	36.0	36.0	36.0	36.0	36.0
30	34.75153649027072	36.0	36.0	36.0	32.0	36.0
31	34.75325923691811	36.0	36.0	36.0	32.0	36.0
32	34.708326508926326	36.0	36.0	36.0	32.0	36.0
33	34.78088610097992	36.0	36.0	36.0	36.0	36.0
34	34.75407267294379	36.0	36.0	36.0	36.0	36.0
35	34.73447680433992	36.0	36.0	36.0	36.0	36.0
36	34.63657136465503	36.0	36.0	36.0	32.0	36.0
37	34.5144495999233	36.0	36.0	36.0	32.0	36.0
38	34.445250612199246	36.0	36.0	36.0	32.0	36.0
39	34.20180453966212	36.0	36.0	36.0	32.0	36.0
40	34.34348122655567	36.0	36.0	36.0	32.0	36.0
41	34.32851280525073	36.0	36.0	36.0	32.0	36.0
42	34.32758052467373	36.0	36.0	36.0	32.0	36.0
43	34.35209346175203	36.0	36.0	36.0	32.0	36.0
44	34.17574512537601	36.0	36.0	36.0	32.0	36.0
45	34.15597098994515	36.0	36.0	36.0	32.0	36.0
46	33.88935472402098	36.0	36.0	36.0	32.0	36.0
47	33.946028095251414	36.0	36.0	36.0	32.0	36.0
48	33.860203853359806	36.0	36.0	36.0	32.0	36.0
49	33.79332632916146	36.0	36.0	36.0	27.0	36.0
50	33.39912094979766	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	6.0
20	10.0
21	28.0
22	121.0
23	366.0
24	1050.0
25	2683.0
26	6231.0
27	12236.0
28	21913.0
29	34070.0
30	50165.0
31	71721.0
32	103271.0
33	165288.0
34	335542.0
35	1197912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.85176055564323	20.890954817032853	12.882509103911435	26.374775523412485
2	9.427951237610536	20.949970763139483	52.169944307650674	17.452133691599307
3	19.5868489555375	26.809277297765068	30.870779068704874	22.733094677992558
4	9.54751185449432	18.191056098623	41.923963455799814	30.33746859108286
5	8.522652370699126	43.08499482676659	37.930936335273465	10.461416467260824
6	28.96965907973049	41.22442274053497	18.937046622680715	10.868871557053824
7	25.508947051729862	32.35939009744759	22.871295780766648	19.260367070055903
8	19.81972579865536	49.0913385292038	19.32102809525141	11.76790757688943
9	26.644292352818393	14.122932556474252	21.054392452988473	38.178382637718876
10	15.013557443111173	30.69010631232554	36.60684413684279	17.689492107720504
11	33.46128264230387	19.08938108953489	31.88489455791824	15.564441710243
12	27.28523819368086	22.6190369187472	36.6446055006107	13.451119386961242
13	32.43242298527317	24.26645507549171	26.215567331334604	17.085554607900512
14	16.69709359576393	28.504922571122115	27.536137804027938	27.261846029086012
15	17.798889907042824	45.192468546117794	21.69075696554617	15.317884581293212
16	15.341225724107213	27.920996862085563	39.692170793626694	17.045606620180525
17	15.947440747502267	34.06484318511387	32.96777814618479	17.019937921199073
18	15.997822841192244	27.506504012263992	42.78416666250543	13.71150648403833
19	24.948417469949305	24.250330567617556	27.89306586984225	22.908186092590892
20	24.01762316038414	30.822504034770937	31.290996868058997	13.868875936785926
21	19.672718084745153	26.908903154673684	27.010270566099543	26.408108194481617
22	22.350215917579806	35.60572770815773	27.740465471163716	14.303590903098748
23	17.12508601292516	35.016623339788254	31.60112373577229	16.257166911514297
24	24.553979826225905	27.553929891141514	33.082442824328375	14.809647458304203
25	19.767949345344505	32.20485573610043	33.02250097374387	15.004693944811192
26	13.258233836820892	34.999767790146215	35.62688420818188	16.11511416485102
27	15.867022493175941	33.56802635140331	33.14944413760178	17.415507017818957
28	14.916642322279733	35.25731392773587	28.732918355168373	21.09312539481602
29	19.033277887664845	28.827336595489477	27.73982914877499	24.399556368070684
30	15.338781103123445	30.666626051418906	32.21886092207266	21.775731923384996
31	22.874398801072882	27.050019051595115	30.814094884434475	19.261487262897532
32	17.389780199586127	33.98826692447207	31.09115205458657	17.530800821355236
33	15.304942814639332	34.43550960463376	35.35095477055694	14.90859281016997
34	17.886935214134372	33.35930021257886	34.447112707741276	14.306651865545497
35	15.599207807668645	35.22484399843399	33.43487084428607	15.741077349611293
36	14.19872846462428	32.53681345104849	35.80554128145317	17.458916802874064
37	15.499611991606635	32.20744316950424	28.85573977770165	23.437205061187473
38	18.364522828932945	36.82702626849184	28.066850542613825	16.741600359961385
39	22.19636587354814	28.42297968007263	30.410228727146887	18.970425719232345
40	24.762703739213805	28.810672339405563	31.997193592201985	14.429430329178652
41	23.12800056728012	25.841407254194426	35.311639529437144	15.718952649088308
42	18.905749394779527	35.20979977788608	32.1867934100878	13.697657417246587
43	14.399146302976554	35.902913435840894	34.2442064852211	15.453733775961458
44	14.047308735187933	35.0158045331296	31.7550771750583	19.18180955662417
45	14.756662257370094	35.471667398220404	31.33871337425041	18.432956970159097
46	17.469576897917197	28.301799170074755	31.783490379957957	22.445133552050095
47	23.35282156582322	29.177868904850552	30.036463035610133	17.432846493716095
48	15.780275005792538	31.40160473310376	29.873373095453054	22.944747165650643
49	21.114480061440066	29.220783760327812	30.089703296011887	19.57503288222023
50	16.422034199608905	36.88362505467902	27.579551741839094	19.11478900387297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20.0
1	26.0
2	32.0
3	75.5
4	119.0
5	174.5
6	230.0
7	263.0
8	296.0
9	421.5
10	547.0
11	848.5
12	1150.0
13	2162.0
14	3174.0
15	4500.5
16	5827.0
17	6900.0
18	7973.0
19	8481.0
20	8989.0
21	10179.5
22	11370.0
23	13254.0
24	15138.0
25	20891.5
26	26645.0
27	44683.0
28	62721.0
29	83866.5
30	105012.0
31	130502.0
32	155992.0
33	201179.0
34	246366.0
35	293247.5
36	340129.0
37	325260.5
38	310392.0
39	263757.0
40	217122.0
41	176842.5
42	136563.0
43	108176.0
44	79789.0
45	68703.5
46	57618.0
47	52327.0
48	47036.0
49	42470.5
50	37905.0
51	35149.5
52	32394.0
53	28886.0
54	25378.0
55	22547.0
56	19716.0
57	17703.5
58	15691.0
59	13344.0
60	10997.0
61	9149.0
62	7301.0
63	5890.0
64	4479.0
65	3916.0
66	3353.0
67	2837.0
68	2321.0
69	1859.0
70	1397.0
71	941.0
72	485.0
73	410.0
74	335.0
75	307.5
76	280.0
77	218.0
78	156.0
79	125.0
80	94.0
81	64.0
82	34.0
83	29.5
84	25.0
85	14.5
86	4.0
87	2.5
88	1.0
89	3.5
90	6.0
91	5.0
92	4.0
93	3.5
94	3.0
95	4.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008988243377662018
2	3.4954279802018964E-4
3	3.4954279802018964E-4
4	0.0
5	0.0
6	3.4954279802018964E-4
7	4.993468543145565E-5
8	0.0
9	0.0010985630794920244
10	0.001298301821217847
11	0.0
12	9.98693708629113E-5
13	5.992162251774679E-4
14	1.997387417258226E-4
15	0.004044709519947908
16	5.992162251774679E-4
17	0.0
18	2.9960811258873394E-4
19	0.0
20	0.0011984324503549358
21	0.0
22	0.0
23	4.993468543145566E-4
24	7.989549669032904E-4
25	0.0017976486755324037
26	0.005842358195480312
27	0.007090725331266703
28	0.005043403228577021
29	0.002746407698730061
30	0.004194513576242276
31	0.00793961498360145
32	0.0035952973510648073
33	0.004643925745125376
34	0.003245754553044618
35	0.0035952973510648073
36	0.007739876241875627
37	0.004094644205379364
38	0.008938308692230562
39	0.00494353385771411
40	0.002796342384161517
41	0.003894905463653541
42	0.0019973874172582264
43	0.001597909933806581
44	0.001298301821217847
45	0.001448105877512214
46	0.001298301821217847
47	0.0019973874172582264
48	0.0019973874172582264
49	6.990855960403793E-4
50	9.986937086291132E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2002616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.2288251077914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56608870216415	32.53741671072288
2	14.145104466778335	12.512427031851562
3	5.022955755812092	6.664782949439616
4	2.3368641335386737	4.1342702025181
5	1.2816371931738535	2.8342653634263515
6	0.7744714438871052	2.0552377225596876
7	0.5124729548155464	1.5866253683706921
8	0.3597892242264952	1.273044373918528
9	0.26119132375995185	1.0396966840426287
>10	1.492075399155012	12.197551678417943
>50	0.1308797113374628	3.9997084864485535
>100	0.10031975666484683	8.685847726469293
>500	0.009894678293827364	3.063158458797038
>1k	0.005686596720590438	5.154174699436402
>5k	4.5492773764723506E-4	1.6549456161330578
>10k+	1.1373193441180876E-4	0.6068469274476964
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	12064	0.6024120450450811	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	9207	0.4597486487674122	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	8913	0.44506785125056425	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	8904	0.4446184390816812	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	5876	0.2934162115952334	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	4711	0.23524230306758762	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	4574	0.22840125116347815	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	4139	0.20667966300079496	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	3696	0.1845585973546601	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	3577	0.17861636978831688	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	3452	0.1723745341093849	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	3301	0.1648343966092351	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	3246	0.16208798891050508	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	3131	0.15634550008588766	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2968	0.14820614636056037	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	2697	0.1346738466086359	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	2612	0.13042939834696218	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	2380	0.11884455132686446	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	2272	0.11345160530026725	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	2234	0.11155408725387193	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	2151	0.10740950836306111	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC	2136	0.10666048808158927	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	2106	0.1051624475186456	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT	2104	0.1050625781477827	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACA	2091	0.10441342723717377	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG	2059	0.1028155173033672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.993468543145565E-5	0.0
11	0.0	0.0	0.0	4.993468543145565E-5	0.0
12	0.0	0.0	0.0	4.993468543145565E-5	0.0
13	0.0	0.0	0.0	4.993468543145565E-5	0.0
14	0.0	0.0	0.0	4.993468543145565E-5	0.0
15	0.0	0.0	0.0	4.993468543145565E-5	0.0
16	0.0	0.0	0.0	4.993468543145565E-5	0.0
17	0.0	0.0	0.0	4.993468543145565E-5	0.0
18	0.0	0.0	0.0	9.98693708629113E-5	0.0
19	0.0	0.0	0.0	9.98693708629113E-5	0.0
20	0.0	0.0	0.0	1.4980405629436697E-4	0.0
21	0.0	0.0	0.0	1.997387417258226E-4	0.0
22	0.0	0.0	0.0	4.993468543145566E-4	0.0
23	0.0	0.0	0.0	8.988243377662018E-4	0.0
24	0.0	0.0	0.0	0.0016977793046694923	0.0
25	0.0	0.0	0.0	0.00229699552984696	0.0
26	0.0	0.0	0.0	0.0026465383278671496	0.0
27	0.0	0.0	0.0	0.0032457545530446175	0.0
28	0.0	0.0	0.0	0.00494353385771411	0.0
29	0.0	0.0	0.0	0.00793961498360145	0.0
30	0.0	0.0	0.0	0.0167780543049691	0.0
31	0.0	0.0	0.0	0.0397480096034387	0.0
32	0.0	0.0	0.0	0.06960895149144919	0.0
33	0.0	0.0	0.0	0.09372740455484227	0.0
34	0.0	0.0	0.0	0.12463697483691331	0.0
35	0.0	0.0	0.0	0.15659517351304494	0.0
36	0.0	0.0	0.0	0.20068750074902028	0.0
37	0.0	0.0	0.0	0.2842282294758456	0.0
38	0.0	0.0	0.0	0.3825995597758132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	4400	0.0	30.447012	4
GACGTGT	175	0.0	26.397408	7
CCTAGAG	655	0.0	25.188995	3
GTCCTAG	735	0.0	25.143528	1
GGACCTG	8295	0.0	24.716078	6
AGGACCT	8540	0.0	24.496426	5
GACGTTA	90	1.5154E-8	24.442045	7
TATAGGA	755	0.0	24.18366	2
GTGCGTA	55	1.5951894E-4	23.997643	9
TACGGCG	130	1.8189894E-12	23.691164	16
CTGTAGG	14080	0.0	23.297497	1
TGTAGGA	14450	0.0	23.170624	2
CGTGTAA	175	0.0	22.62635	9
CTAGAGT	780	0.0	22.561888	4
GTCGAGG	440	0.0	22.498354	19
GTAAGAC	235	0.0	22.466442	3
TAGAGTG	785	0.0	22.41818	5
ATAGGAC	775	0.0	22.140314	3
ACTGTTC	3425	0.0	22.029955	8
GTAGGAC	14845	0.0	22.020618	3
>>END_MODULE