FastQCFastQC Report
Thu 2 Feb 2017
SRR4063918_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063918_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872520
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34830.39918855728235453No Hit
TATCAACGCAGAGTACTTTTTTTTT25800.2956952276165589No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23300.26704258928162106No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14160.16228854352908817No Hit
GTACATGGGGTGGTATCAACGCAAA13420.15380736258194655No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13170.15094209874845277No Hit
GGTATCAACGCAGAGTACTTTTTTT13170.15094209874845277No Hit
GTCCTAAAGTGTGTATTTCTCATTT10500.1203410810067391No Hit
CTTTAGGACGTGAAATATGGCGAGG9220.1056709301792509No Hit
GTCCTACAGTGTGCATTTCTCATTT8940.10246183468573787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT402.764842E-416.6206864
TCGAACG456.7773217E-414.7722483
ATTATAC551.9648265E-413.8130123
ACGGTAT1257.2759576E-1213.6788039
GCGTTAT707.2732746E-613.5679061
CCTATAC655.466296E-513.1489253
GTCTTGG751.48076815E-512.663381
GTATAAA905.412858E-712.6633791
TACGCTA1103.8095095E-812.0898519
ATATACG1103.81242E-812.0891586
ACAGCGG550.003069917212.0891586
TAGGACC4650.012.0506354
GTATTGA802.8705957E-511.8719191
GACGTGA3300.011.8019987
TAATATA1459.822543E-1111.7900434
TTAGGAC3500.011.6670623
CGAGCCG1552.5465852E-1111.64881815
GGACGTG4900.011.6309676
AGAGCGA1157.0260285E-811.568850515
TGTAGAA3150.011.456032
Produced by FastQC (version 0.11.5)