##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063918_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	872520
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211898867647733	32.0	32.0	32.0	32.0	32.0
2	30.854731123641866	32.0	32.0	32.0	32.0	32.0
3	30.877073304909917	32.0	32.0	32.0	32.0	32.0
4	30.900905423371384	32.0	32.0	32.0	32.0	32.0
5	30.834379727685324	32.0	32.0	32.0	32.0	32.0
6	34.479350616604776	36.0	36.0	36.0	32.0	36.0
7	34.39273827534039	36.0	36.0	36.0	32.0	36.0
8	34.34558061706322	36.0	36.0	36.0	32.0	36.0
9	34.47006143125659	36.0	36.0	36.0	32.0	36.0
10	34.20165497639022	36.0	36.0	36.0	32.0	36.0
11	34.47051758125888	36.0	36.0	36.0	32.0	36.0
12	34.293406454866364	36.0	36.0	36.0	32.0	36.0
13	34.37668935955623	36.0	36.0	36.0	32.0	36.0
14	34.287327511117226	36.0	36.0	36.0	32.0	36.0
15	34.24460986567643	36.0	36.0	36.0	32.0	36.0
16	34.23694815018567	36.0	36.0	36.0	32.0	36.0
17	34.16107710998029	36.0	36.0	36.0	32.0	36.0
18	34.15816600192546	36.0	36.0	36.0	32.0	36.0
19	34.150247558795215	36.0	36.0	36.0	32.0	36.0
20	34.12759592903315	36.0	36.0	36.0	32.0	36.0
21	34.0952608536194	36.0	36.0	36.0	32.0	36.0
22	34.08010131572915	36.0	36.0	36.0	32.0	36.0
23	34.05562967038005	36.0	36.0	36.0	32.0	36.0
24	34.02345390363545	36.0	36.0	36.0	32.0	36.0
25	33.64560010085729	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	9.0
5	11.0
6	44.0
7	14.0
8	66.0
9	59.0
10	72.0
11	25.0
12	58.0
13	60.0
14	114.0
15	204.0
16	313.0
17	390.0
18	465.0
19	661.0
20	979.0
21	1396.0
22	2227.0
23	3237.0
24	4731.0
25	6701.0
26	9595.0
27	12447.0
28	17188.0
29	23108.0
30	30428.0
31	41276.0
32	58831.0
33	82702.0
34	178603.0
35	396501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.407556632319036	17.36284929044064	11.49673053484391	25.732863542396416
2	15.707534789042182	20.126582713734408	39.48798415538361	24.677898341839803
3	18.94269946877095	24.97888264250635	28.548375080945092	27.530042807777605
4	12.117499272191289	15.954942336919272	36.5143349662228	35.41322342466664
5	13.67318376521481	37.69490258739167	34.01778724311487	14.614126404278657
6	32.89231908462224	36.19281897973711	17.641792652997594	13.27306928264306
7	29.34596817368396	30.7152265799378	21.328286122731132	18.61051912364711
8	27.324614155154286	34.191339601938594	19.718703886940382	18.765342355966734
9	27.682913544288557	14.198482911250881	18.863846158256553	39.254757386204005
10	15.862406690207475	27.338952086159818	32.23573072648892	24.562910497143793
11	36.29996857790958	21.30004885332306	23.555558613666545	18.844423955100815
12	24.973362679307304	23.81680404910213	29.94161007550204	21.268223196088528
13	29.815428409550975	20.285097905634863	25.478861948065802	24.42061173674836
14	22.971931402145287	20.605163190589355	26.06105998183203	30.361845425433327
15	24.075354759389814	28.84615605169687	23.019878912159133	24.058610276754187
16	24.066064516129032	26.13367741935484	25.938924731182794	23.861333333333334
17	22.75525745953683	26.572764891547695	26.56599809153301	24.105979557382465
18	23.22924696252248	25.56883878427486	28.332966266563886	22.86894798663877
19	25.082846691466187	24.827280850570837	26.232663562711707	23.85720889525127
20	25.250962150628908	25.09036472821754	26.196192693964406	23.462480427189146
21	25.811384038670216	24.46024350124228	25.344983034749514	24.383389425337988
22	25.180036844749626	25.54986085715662	25.792930671762836	23.47717162633092
23	23.629119867480536	25.480400265681336	26.614019852682226	24.276460014155905
24	24.416231834850706	25.500204750439607	26.59301003793332	23.49055337677637
25	23.919686527855525	25.61506061134409	26.714415497416795	23.75083736338359
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	335.0
1	335.0
2	561.0
3	787.0
4	787.0
5	787.0
6	1883.0
7	2979.0
8	2979.0
9	2979.0
10	2819.0
11	2659.0
12	2659.0
13	2659.0
14	2593.5
15	2528.0
16	2528.0
17	2528.0
18	4382.0
19	6236.0
20	6236.0
21	6236.0
22	10588.0
23	14940.0
24	14940.0
25	14940.0
26	23373.0
27	31806.0
28	31806.0
29	31806.0
30	41011.5
31	50217.0
32	50217.0
33	50217.0
34	57011.0
35	63805.0
36	63805.0
37	63805.0
38	70533.0
39	77261.0
40	77261.0
41	77261.0
42	85921.0
43	94581.0
44	94581.0
45	94581.0
46	103817.5
47	113054.0
48	113054.0
49	113054.0
50	113698.5
51	114343.0
52	114343.0
53	114343.0
54	103706.0
55	93069.0
56	93069.0
57	93069.0
58	84718.5
59	76368.0
60	76368.0
61	76368.0
62	66457.5
63	56547.0
64	56547.0
65	56547.0
66	45913.0
67	35279.0
68	35279.0
69	35279.0
70	26334.5
71	17390.0
72	17390.0
73	17390.0
74	13176.0
75	8962.0
76	8962.0
77	8962.0
78	7097.0
79	5232.0
80	5232.0
81	5232.0
82	3572.5
83	1913.0
84	1913.0
85	1913.0
86	1488.0
87	1063.0
88	1063.0
89	1063.0
90	756.0
91	449.0
92	449.0
93	449.0
94	328.0
95	207.0
96	207.0
97	207.0
98	358.5
99	510.0
100	510.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0411451886489708
2	0.005386696006968322
3	0.0017191583000962728
4	0.0043552010269105575
5	0.011117223673955897
6	0.01570164580754596
7	0.025558153394764593
8	0.03931141979553477
9	0.05214780176958694
10	0.06406729931692111
11	0.05982670884335029
12	0.0714023747306652
13	0.06979782698390867
14	0.075184522990877
15	0.06773483702379315
16	0.07392380690413973
17	0.07060010085728693
18	0.08527025168477513
19	0.08355109338467887
20	0.08882317883830743
21	0.08458258836473663
22	0.08641635721817265
23	0.09157383211846146
24	0.08332187227799935
25	0.08435336725805713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	872520.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.447983138541275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19378449350062	40.94149910225228
2	14.785040327180859	16.100312528739927
3	4.774399892312831	7.79869334499907
4	1.9339564904606186	4.212001215330889
5	0.9685788962518543	2.6368583705733943
6	0.5754419474355751	1.8798992070708929
7	0.35912317355871276	1.3687472749008955
8	0.2323007792453946	1.0118647129138596
9	0.16612727921542486	0.8140765767815873
>10	0.837462049532665	8.368089566476609
>50	0.09123322652705565	3.518344195360595
>100	0.0755663225504881	7.79817752289714
>500	0.005291759263547699	1.8416989576378953
>1k	0.0016933629643352639	1.7097374240649676
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3483	0.39918855728235453	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2580	0.2956952276165589	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2330	0.26704258928162106	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1416	0.16228854352908817	No Hit
GTACATGGGGTGGTATCAACGCAAA	1342	0.15380736258194655	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1317	0.15094209874845277	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1317	0.15094209874845277	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1050	0.1203410810067391	No Hit
CTTTAGGACGTGAAATATGGCGAGG	922	0.1056709301792509	No Hit
GTCCTACAGTGTGCATTTCTCATTT	894	0.10246183468573787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1461055333975153E-4
2	0.0	0.0	0.0	0.0	1.1461055333975153E-4
3	0.0	0.0	0.0	0.0	1.1461055333975153E-4
4	0.0	0.0	0.0	0.0	1.1461055333975153E-4
5	0.0	0.0	0.0	0.0	2.2922110667950306E-4
6	0.0	0.0	0.0	0.0	3.438316600192546E-4
7	0.0	0.0	0.0	0.0	3.438316600192546E-4
8	0.0	0.0	0.0	0.0	3.438316600192546E-4
9	0.0	0.0	0.0	1.1461055333975153E-4	3.438316600192546E-4
10	0.0	0.0	0.0	1.1461055333975153E-4	3.438316600192546E-4
11	0.0	0.0	0.0	1.1461055333975153E-4	3.438316600192546E-4
12	0.0	0.0	0.0	1.1461055333975153E-4	5.730527666987577E-4
13	0.0	0.0	0.0	1.1461055333975153E-4	6.876633200385092E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	40	2.764842E-4	16.620686	4
TCGAACG	45	6.7773217E-4	14.772248	3
ATTATAC	55	1.9648265E-4	13.813012	3
ACGGTAT	125	7.2759576E-12	13.678803	9
GCGTTAT	70	7.2732746E-6	13.567906	1
CCTATAC	65	5.466296E-5	13.148925	3
GTCTTGG	75	1.48076815E-5	12.66338	1
GTATAAA	90	5.412858E-7	12.663379	1
TACGCTA	110	3.8095095E-8	12.089851	9
ATATACG	110	3.81242E-8	12.089158	6
ACAGCGG	55	0.0030699172	12.089158	6
TAGGACC	465	0.0	12.050635	4
GTATTGA	80	2.8705957E-5	11.871919	1
GACGTGA	330	0.0	11.801998	7
TAATATA	145	9.822543E-11	11.790043	4
TTAGGAC	350	0.0	11.667062	3
CGAGCCG	155	2.5465852E-11	11.648818	15
GGACGTG	490	0.0	11.630967	6
AGAGCGA	115	7.0260285E-8	11.5688505	15
TGTAGAA	315	0.0	11.45603	2
>>END_MODULE