FastQCFastQC Report
Thu 2 Feb 2017
SRR4063918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872520
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9500.10888002567276393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.10865080456608443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG300.00574250229.33505430
CAGTTCG300.005745719729.3316929
ACGCATG450.001398358424.44307516
GTATTAG2150.022.5129471
CCGTCGT1451.2732926E-1121.240199
CGAGCCG1800.020.77661315
CGTCGTA1653.6379788E-1219.9988810
ATACCGT1705.456968E-1219.4117896
TGTAGGA9300.019.1613162
GTAGGAC9600.019.020863
GTCCTAC8450.018.746661
GTCCTAA4850.018.5990281
TCCTACA9100.018.373652
ATTTCGT3600.018.33335742
GTTCGCA600.007412883518.3323089
AGGCCCG2050.018.24288210
GATTTCG4000.018.15002341
TAGGACC7400.018.1351594
GTGTTAA851.4296132E-418.1187081
ACCGTCG1701.2732926E-1018.1166328
Produced by FastQC (version 0.11.5)