##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063918_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	872520
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23343304451474	32.0	32.0	32.0	32.0	32.0
2	31.373009214688487	32.0	32.0	32.0	32.0	32.0
3	31.469075780497867	32.0	32.0	32.0	32.0	32.0
4	31.557517306193553	32.0	32.0	32.0	32.0	32.0
5	31.497948471095217	32.0	32.0	32.0	32.0	32.0
6	35.077257827900794	36.0	36.0	36.0	36.0	36.0
7	35.109714390501075	36.0	36.0	36.0	36.0	36.0
8	35.05035300050429	36.0	36.0	36.0	36.0	36.0
9	35.15918374363911	36.0	36.0	36.0	36.0	36.0
10	35.016916517672946	36.0	36.0	36.0	36.0	36.0
11	35.16207422179434	36.0	36.0	36.0	36.0	36.0
12	35.07662746985743	36.0	36.0	36.0	36.0	36.0
13	35.117701599963326	36.0	36.0	36.0	36.0	36.0
14	35.061674230963185	36.0	36.0	36.0	36.0	36.0
15	35.027818273506625	36.0	36.0	36.0	36.0	36.0
16	35.04562302296795	36.0	36.0	36.0	36.0	36.0
17	35.014095951955255	36.0	36.0	36.0	36.0	36.0
18	35.00459588318893	36.0	36.0	36.0	36.0	36.0
19	35.00203433732178	36.0	36.0	36.0	36.0	36.0
20	34.99479553477284	36.0	36.0	36.0	36.0	36.0
21	34.98715903360382	36.0	36.0	36.0	36.0	36.0
22	34.97056113326915	36.0	36.0	36.0	36.0	36.0
23	34.919570898088296	36.0	36.0	36.0	36.0	36.0
24	34.908502956952276	36.0	36.0	36.0	32.0	36.0
25	34.8719490670701	36.0	36.0	36.0	32.0	36.0
26	34.81991014532618	36.0	36.0	36.0	32.0	36.0
27	34.79832324760464	36.0	36.0	36.0	32.0	36.0
28	34.77090381882364	36.0	36.0	36.0	32.0	36.0
29	34.737807729335714	36.0	36.0	36.0	32.0	36.0
30	34.71722482006143	36.0	36.0	36.0	32.0	36.0
31	34.71653944895246	36.0	36.0	36.0	32.0	36.0
32	34.662217484986016	36.0	36.0	36.0	32.0	36.0
33	34.623532984917254	36.0	36.0	36.0	32.0	36.0
34	34.59604937422638	36.0	36.0	36.0	32.0	36.0
35	34.56187479943153	36.0	36.0	36.0	32.0	36.0
36	34.5262561316646	36.0	36.0	36.0	32.0	36.0
37	34.49730321367991	36.0	36.0	36.0	32.0	36.0
38	34.45014899371934	36.0	36.0	36.0	32.0	36.0
39	34.42043735387154	36.0	36.0	36.0	32.0	36.0
40	34.41313207720167	36.0	36.0	36.0	32.0	36.0
41	34.38906729931692	36.0	36.0	36.0	32.0	36.0
42	34.32125796543346	36.0	36.0	36.0	32.0	36.0
43	34.307082932196394	36.0	36.0	36.0	32.0	36.0
44	34.225730069224774	36.0	36.0	36.0	32.0	36.0
45	34.172365103378716	36.0	36.0	36.0	32.0	36.0
46	34.11844427634897	36.0	36.0	36.0	32.0	36.0
47	34.07395475175354	36.0	36.0	36.0	32.0	36.0
48	34.00631274927795	36.0	36.0	36.0	32.0	36.0
49	33.95983129326548	36.0	36.0	36.0	32.0	36.0
50	33.38960482281208	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	1.0
20	11.0
21	29.0
22	88.0
23	300.0
24	747.0
25	1811.0
26	3587.0
27	6665.0
28	10872.0
29	16096.0
30	23102.0
31	32374.0
32	46893.0
33	74852.0
34	153653.0
35	501438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75103877124108	18.266555864400953	12.025331690403187	25.957073673954785
2	15.102725674427347	20.89366637708334	38.89020054600593	25.11340740248339
3	18.301941286901986	25.47913271305675	28.908189438794107	27.310736561247158
4	11.81760876541512	16.642827671572	36.55881813597396	34.980745427038926
5	13.367945720441938	38.0518498143309	33.74742126254986	14.832783202677302
6	32.973800027506535	36.50254435428415	17.36177967267226	13.161875945537066
7	29.201379457363007	30.938537587233256	21.282336046174457	18.577746909229276
8	27.240407096685466	34.42259203227433	19.560239306835374	18.776761564204833
9	27.63588143132376	14.229341426487121	18.671254213433244	39.46352292875587
10	15.716424034456752	27.379274143452136	32.260685788422876	24.643616033668227
11	36.40260395177188	21.205129968367487	23.615275294549125	18.77699078531151
12	24.99014349241278	23.81504148902031	29.979713932058864	21.215101086508046
13	29.9261790887742	20.178861844525667	25.53474602787109	24.36021303882904
14	22.997947317931914	20.598223301093274	25.970726071812926	30.433103309161886
15	24.277889273871974	28.794111478010308	22.957396011585406	23.97060323653231
16	24.25757042636907	26.176867348915252	25.73710010349487	23.828462121220802
17	22.816325127217716	26.71686608902948	26.345413285655344	24.121395498097463
18	23.43928721241281	25.49815591196972	28.34336942045895	22.71918745515852
19	25.19326226706811	24.865934151577214	26.371803937793903	23.56899964356077
20	25.3359266118976	24.95071701019585	26.454650480451846	23.258705897454707
21	25.951611424379955	24.369641956631366	25.509673130701877	24.169073488286802
22	25.251599105578215	25.36185458425547	25.906026115190617	23.480520194975696
23	23.574898827186175	25.323403440849862	26.774492645988555	24.327205085975404
24	24.487716543555123	25.30351913720681	26.80698899159327	23.401775327644796
25	24.087280029157526	25.29633169895312	26.963261975330717	23.653126296558632
26	23.353804045085997	26.238844837506566	27.26455816043261	23.142792956974827
27	23.935556266197185	25.736459698023157	26.501431025925807	23.82655300985385
28	23.321142073828373	25.845431945378877	26.701723701206788	24.131702279585962
29	23.6857517808125	25.331033415664272	26.408403486552935	24.574811316970298
30	23.147913612664457	25.892933893722443	26.99346352790694	23.965688965706157
31	24.4174757837958	25.34531946133488	25.9960135388382	24.241191216031122
32	23.60264393624203	26.12027457142628	26.139529872651394	24.13755161968029
33	23.441022649255743	25.728412947474954	26.854743781798764	23.975820621470547
34	24.05472129321233	25.75238339747895	26.863909665854436	23.32898564345428
35	24.119280639360365	25.833326647541483	26.876997857872293	23.17039485522586
36	23.281283849071396	26.240229975963935	26.480705241283342	23.997780933681323
37	23.981578905141447	26.128223290443998	25.834118453416586	24.056079350997962
38	23.728574605190463	26.481982839052502	26.04356028911818	23.74588226663885
39	24.48695157831517	25.66267918728316	25.796894144399708	24.05347509000196
40	24.819426520175405	25.87077563501578	26.354673592374994	22.955124252433816
41	23.873112876679674	25.852386039069696	26.972396308504837	23.302104775745793
42	24.468400899016515	26.841786159225865	26.115833762173228	22.573979179584395
43	23.56170538068948	26.08912916657593	26.5873475230628	23.761817929671786
44	23.289906946731904	26.526886193984943	25.930675945632775	24.25253091365037
45	23.29835371136998	26.621188573629123	26.059130689919176	24.021327025081717
46	23.45856164893818	25.805002332343474	25.948266673390542	24.788169345327805
47	24.310744195695353	25.88094656842014	26.0637548008651	23.74455443501941
48	24.224329314627	26.385468015582752	25.23040035392429	24.159802315865964
49	23.860334779344768	26.4612069706538	25.54752640355753	24.1309318464439
50	23.693765558596596	27.16020941928326	25.122520366575436	24.023504655544706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	84.0
2	36.0
3	120.0
4	204.0
5	209.0
6	214.0
7	215.5
8	217.0
9	342.0
10	467.0
11	650.0
12	833.0
13	1493.0
14	2153.0
15	2910.5
16	3668.0
17	4064.5
18	4461.0
19	4503.5
20	4546.0
21	4738.5
22	4931.0
23	5477.0
24	6023.0
25	6365.0
26	6707.0
27	8531.0
28	10355.0
29	12763.0
30	15171.0
31	17850.0
32	20529.0
33	24669.0
34	28809.0
35	33126.5
36	37444.0
37	40150.5
38	42857.0
39	43376.5
40	43896.0
41	44919.0
42	45942.0
43	46328.5
44	46715.0
45	50409.0
46	54103.0
47	56651.0
48	59199.0
49	60289.0
50	61379.0
51	59523.0
52	57667.0
53	56923.5
54	56180.0
55	55807.0
56	55434.0
57	54057.5
58	52681.0
59	47992.5
60	43304.0
61	37874.5
62	32445.0
63	28006.5
64	23568.0
65	20039.0
66	16510.0
67	13977.0
68	11444.0
69	10133.0
70	8822.0
71	6872.5
72	4923.0
73	4139.0
74	3355.0
75	2589.5
76	1824.0
77	1565.0
78	1306.0
79	1067.5
80	829.0
81	687.0
82	545.0
83	450.5
84	356.0
85	262.5
86	169.0
87	113.5
88	58.0
89	46.0
90	34.0
91	25.5
92	17.0
93	15.5
94	14.0
95	18.0
96	22.0
97	16.5
98	11.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010888002567276395
2	2.2922110667950306E-4
3	6.876633200385092E-4
4	0.0
5	0.0
6	0.0
7	3.438316600192546E-4
8	0.0
9	0.0014899371934167698
10	6.876633200385092E-4
11	0.0
12	0.0
13	3.438316600192546E-4
14	3.438316600192546E-4
15	0.004928253793609316
16	0.0014899371934167698
17	0.0
18	2.2922110667950306E-4
19	1.1461055333975153E-4
20	9.168844267180122E-4
21	0.0
22	1.1461055333975153E-4
23	3.438316600192546E-4
24	5.730527666987575E-4
25	0.0020629899601155278
26	0.006188969880346582
27	0.007908128180442855
28	0.0042405904735708065
29	0.002865263833493788
30	0.003552927153532297
31	0.007678907073763353
32	0.0037821482602118
33	0.004699032686929812
34	0.0029798743868335396
35	0.0026360427268142853
36	0.009283454820519874
37	0.004813643240269564
38	0.008481180947141613
39	0.004469811580250309
40	0.0025214321734745334
41	0.003209095493513043
42	0.0012607160867372667
43	0.0012607160867372667
44	0.0010314949800577639
45	0.0013753266400770184
46	8.022738733782607E-4
47	0.002406821620134782
48	0.001948379406775776
49	5.730527666987575E-4
50	0.0013753266400770184
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	872520.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.18582492726002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.34354193267923	45.184555071544864
2	14.396263061260067	17.041094103010373
3	4.496027917183857	7.983033635235519
4	1.859497241845282	4.40223512834311
5	0.9177045968226536	2.715755180124366
6	0.5124801101964085	1.8198934848472532
7	0.30134332010924597	1.2484677090889578
8	0.20609803024547363	0.9758465548769394
9	0.1406123369307545	0.7490031440576881
>10	0.6960562946024689	7.460491721129932
>50	0.0684054694519669	2.832273293868176
>100	0.058851716955648635	6.442166623251959
>500	0.00311797171698028	1.1451843506208803
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	950	0.10888002567276393	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	948	0.10865080456608443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	1.1461055333975153E-4
12	0.0	0.0	0.0	0.0	2.2922110667950306E-4
13	0.0	0.0	0.0	0.0	3.438316600192546E-4
14	0.0	0.0	0.0	0.0	3.438316600192546E-4
15	0.0	0.0	0.0	0.0	3.438316600192546E-4
16	0.0	0.0	0.0	3.438316600192546E-4	3.438316600192546E-4
17	0.0	0.0	0.0	3.438316600192546E-4	3.438316600192546E-4
18	0.0	0.0	0.0	3.438316600192546E-4	3.438316600192546E-4
19	0.0	0.0	0.0	5.730527666987577E-4	3.438316600192546E-4
20	0.0	0.0	0.0	5.730527666987577E-4	4.584422133590061E-4
21	0.0	0.0	0.0	9.168844267180122E-4	4.584422133590061E-4
22	0.0	0.0	0.0	0.0011461055333975153	4.584422133590061E-4
23	0.0	0.0	0.0	0.002406821620134782	4.584422133590061E-4
24	0.0	0.0	0.0	0.003552927153532297	4.584422133590061E-4
25	0.0	0.0	0.0	0.004699032686929812	4.584422133590061E-4
26	0.0	0.0	0.0	0.0055013065603080735	4.584422133590061E-4
27	0.0	0.0	0.0	0.007105854307064594	4.584422133590061E-4
28	0.0	0.0	0.0	0.010314949800577638	4.584422133590061E-4
29	0.0	0.0	0.0	0.016618530234263972	4.584422133590061E-4
30	0.0	0.0	0.0	0.02773575390821987	4.584422133590061E-4
31	0.0	0.0	0.0	0.056044560583138496	4.584422133590061E-4
32	0.0	0.0	0.0	0.08125888231788383	4.584422133590061E-4
33	0.0	0.0	0.0	0.10555631962591115	4.584422133590061E-4
34	0.0	0.0	0.0	0.13317746298079128	6.876633200385092E-4
35	0.0	0.0	0.0	0.16515380736258195	6.876633200385092E-4
36	0.0	0.0	0.0	0.20950809150506577	6.876633200385092E-4
37	0.0	0.0	0.0	0.2775867601888782	6.876633200385092E-4
38	0.0	0.0	0.0	0.36961903452069866	8.022738733782607E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	30	0.005742502	29.335054	30
CAGTTCG	30	0.0057457197	29.331692	9
ACGCATG	45	0.0013983584	24.443075	16
GTATTAG	215	0.0	22.512947	1
CCGTCGT	145	1.2732926E-11	21.24019	9
CGAGCCG	180	0.0	20.776613	15
CGTCGTA	165	3.6379788E-12	19.99888	10
ATACCGT	170	5.456968E-12	19.411789	6
TGTAGGA	930	0.0	19.161316	2
GTAGGAC	960	0.0	19.02086	3
GTCCTAC	845	0.0	18.74666	1
GTCCTAA	485	0.0	18.599028	1
TCCTACA	910	0.0	18.37365	2
ATTTCGT	360	0.0	18.333357	42
GTTCGCA	60	0.0074128835	18.332308	9
AGGCCCG	205	0.0	18.242882	10
GATTTCG	400	0.0	18.150023	41
TAGGACC	740	0.0	18.135159	4
GTGTTAA	85	1.4296132E-4	18.118708	1
ACCGTCG	170	1.2732926E-10	18.116632	8
>>END_MODULE