FastQCFastQC Report
Thu 2 Feb 2017
SRR4063917_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063917_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065399
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57410.2779608201611408No Hit
GCGCAAGACGGACCAGAGCGAAAGC42450.2055292948239057No Hit
TATCAACGCAGAGTACTTTTTTTTT39570.19158525786058772No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG34570.16737686035482732No Hit
GTCTTGCGCCGGTCCAAGAATTTCA34040.16481077021921672No Hit
GAATAGGACCGCGGTTCTATTTTGT33590.1626320144436983No Hit
GTATCTGATCGTCTTCGAACCTCCG32830.15895233802282271No Hit
GAACTACGACGGTATCTGATCGTCT32510.15740300058245404No Hit
GGGTAGGCACACGCTGAGCCAGTCA29430.14249062771890564No Hit
GATTAAGAGGGACGGCCGGGGGCAT29420.14244221092389414No Hit
CTATTGGAGCTGGAATTACCGCGGC28530.13813311616786877No Hit
GTCCTATTCCATTATTCCTAGCTGC28450.1377457818077766No Hit
ATCAGATACCGTCGTAGTTCCGACC27880.13498602449211994No Hit
GAATAACGCCGCCGCATCGCCAGTC27070.13106426409618674No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27050.1309674305061637No Hit
GTGCATGGCCGTTCTTAGTTGGTGG26220.12694883652020747No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26080.12627100139004618No Hit
TCGTAGTTCCGACCATAAACGATGC25400.12297865932926277No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG24600.11910531572834113No Hit
CTCTTAATCATGGCCTCAGTTCCGA23940.11590980725758075No Hit
GTCCTGTATTGTTATTTTTCGTCAC23610.11431205302220054No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC23400.11329530032695861No Hit
CTTTAATATACGCTATTGGAGCTGG22890.11082604378137106No Hit
GCTTTGAACACTCTAATTTTTTCAA22170.10734003454054156No Hit
GTACATGGGGAATAATTGCAATCCC21960.10632328184529963No Hit
GAATAATGGAATAGGACCGCGGTTC21830.10569386351014987No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT21700.10506444517500008No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20880.10109426798405538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2350.012.9339111
CGGTCCA6650.012.85863610
TCGCGTA2300.012.3927439
AAGACGG8300.012.3607855
TAGGGTG853.947447E-612.293465
CAAGACG8600.012.2600794
GTATCAA33950.012.1701871
AGGCCCG6450.012.07892110
GCACGCC3150.012.06430715
TATACCG951.040984E-611.9993585
CGCAAGA8500.011.9549982
GGTCGGC4000.011.8760911
AGAATTT7250.011.79361517
GAGGTTA2500.011.7793695
GGTTCTA6550.011.74860113
TCCATTA5500.011.7468178
ACGGTAT6150.011.74119959
CGGTCGG4050.011.72975510
ACAATAC2350.011.72022153
CGGACCA8200.011.7025789
Produced by FastQC (version 0.11.5)