##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063917_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2065399
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2351279341183	32.0	32.0	32.0	32.0	32.0
2	30.991824824162304	32.0	32.0	32.0	32.0	32.0
3	31.00453423285283	32.0	32.0	32.0	32.0	32.0
4	31.003378039787954	32.0	32.0	32.0	32.0	32.0
5	30.977276061429293	32.0	32.0	32.0	32.0	32.0
6	34.49687009628648	36.0	36.0	36.0	32.0	36.0
7	34.46085284247741	36.0	36.0	36.0	32.0	36.0
8	34.4515606911788	36.0	36.0	36.0	32.0	36.0
9	34.534196540232664	36.0	36.0	36.0	32.0	36.0
10	34.341105035879266	36.0	36.0	36.0	32.0	36.0
11	34.47757745597824	36.0	36.0	36.0	32.0	36.0
12	34.372374538769506	36.0	36.0	36.0	32.0	36.0
13	34.4484218303582	36.0	36.0	36.0	32.0	36.0
14	34.35830558647506	36.0	36.0	36.0	32.0	36.0
15	34.31419110786826	36.0	36.0	36.0	32.0	36.0
16	34.298106564397486	36.0	36.0	36.0	32.0	36.0
17	34.22346529653592	36.0	36.0	36.0	32.0	36.0
18	34.209905688925	36.0	36.0	36.0	32.0	36.0
19	34.22978078327723	36.0	36.0	36.0	32.0	36.0
20	34.19844446520987	36.0	36.0	36.0	32.0	36.0
21	34.166324279231276	36.0	36.0	36.0	32.0	36.0
22	34.16085947557833	36.0	36.0	36.0	32.0	36.0
23	34.12456334102999	36.0	36.0	36.0	32.0	36.0
24	34.1122059224392	36.0	36.0	36.0	32.0	36.0
25	33.61644699159823	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	16.0
5	26.0
6	86.0
7	51.0
8	119.0
9	133.0
10	178.0
11	53.0
12	128.0
13	105.0
14	223.0
15	456.0
16	759.0
17	1088.0
18	1394.0
19	1710.0
20	2447.0
21	3132.0
22	4525.0
23	6416.0
24	9086.0
25	12746.0
26	18232.0
27	25004.0
28	35093.0
29	48356.0
30	66913.0
31	96368.0
32	145203.0
33	219022.0
34	490973.0
35	875353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.890887674043405	16.790747843573925	11.079058219750339	26.23930626263233
2	17.308886033217675	19.01063581508808	36.05035815862791	27.630119993066337
3	19.501591720631847	22.760564062216304	27.574459843854022	30.163384373297824
4	12.829300154406543	14.260889437846888	34.269875908270215	38.639934499476354
5	15.473862807703592	35.63580212839516	32.37929361452399	16.511041449377256
6	36.24241508600715	34.064622910350444	15.840830479986867	13.852131523655537
7	31.7192186193193	29.568179517838317	19.983108214454003	18.729493648388377
8	29.430235058672643	31.435150115562926	18.678862930551816	20.455751895212614
9	27.2452747565747	13.746184710163917	18.17334991287988	40.8351906203815
10	16.32440398332723	25.592286421303324	29.965110037573805	28.11819955779564
11	38.57956152895172	20.403252684734124	21.387913561443693	19.629272224870462
12	25.627265235487595	22.788420404451575	27.524673231983854	24.05964112807698
13	29.32049794460984	18.274441217294832	25.5373204107389	26.86774042735643
14	24.43641479870815	18.886947691347654	23.977213377015122	32.699424132929074
15	25.702601895502696	26.40744442382793	21.597520334045655	26.292433346623717
16	26.882133551419706	24.9576795506232	22.687346112962413	25.472840784994677
17	24.73422925636515	25.31739152973391	24.290208135203343	25.658171078697595
18	26.108801167564614	23.70743435719744	25.318795629191637	24.864968846046313
19	26.072496442333833	23.983587978407378	24.813443593124124	25.130471986134673
20	26.527487309300312	23.089901905107098	24.02297602964749	26.3596347559451
21	27.870607878612276	23.117973744419455	23.8205387365265	25.19087964044177
22	26.788750506959826	22.90697668784309	24.456759537022723	25.84751326817436
23	24.68550312473803	22.81264703616284	25.485573677867784	27.016276161231346
24	25.427208481421452	24.003624373426753	24.547280387439706	26.021886757712092
25	25.426086670006814	23.46381546112913	24.668157780230665	26.44194008863339
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	125.0
2	366.0
3	607.0
4	607.0
5	607.0
6	1893.5
7	3180.0
8	3180.0
9	3180.0
10	3354.0
11	3528.0
12	3528.0
13	3528.0
14	3403.0
15	3278.0
16	3278.0
17	3278.0
18	5642.0
19	8006.0
20	8006.0
21	8006.0
22	14078.5
23	20151.0
24	20151.0
25	20151.0
26	30583.5
27	41016.0
28	41016.0
29	41016.0
30	51320.0
31	61624.0
32	61624.0
33	61624.0
34	83349.0
35	105074.0
36	105074.0
37	105074.0
38	125899.5
39	146725.0
40	146725.0
41	146725.0
42	176555.0
43	206385.0
44	206385.0
45	206385.0
46	236577.5
47	266770.0
48	266770.0
49	266770.0
50	280121.0
51	293472.0
52	293472.0
53	293472.0
54	275920.5
55	258369.0
56	258369.0
57	258369.0
58	240862.0
59	223355.0
60	223355.0
61	223355.0
62	200047.5
63	176740.0
64	176740.0
65	176740.0
66	146224.0
67	115708.0
68	115708.0
69	115708.0
70	87608.0
71	59508.0
72	59508.0
73	59508.0
74	46176.0
75	32844.0
76	32844.0
77	32844.0
78	27991.0
79	23138.0
80	23138.0
81	23138.0
82	15598.0
83	8058.0
84	8058.0
85	8058.0
86	6250.5
87	4443.0
88	4443.0
89	4443.0
90	3115.0
91	1787.0
92	1787.0
93	1787.0
94	1151.5
95	516.0
96	516.0
97	516.0
98	754.0
99	992.0
100	992.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03660309702870971
2	0.0054710978363018475
3	0.0011620030802764988
4	0.003486009240829496
5	0.00876343989708526
6	0.013556702603225817
7	0.02372422955564518
8	0.03500534279332952
9	0.04880412937161294
10	0.05785807003876733
11	0.054953062338076085
12	0.06391016941520743
13	0.06361966864513831
14	0.0668635939109102
15	0.060520993764400975
16	0.06705726109095628
17	0.06332916787506918
18	0.07824154073861757
19	0.07485236508781112
20	0.08148546600438947
21	0.07422294675266135
22	0.07872570868873278
23	0.08259905228965445
24	0.07717637124836411
25	0.07828995753362909
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2065399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.04930676691064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.69261042353857	24.042223722442042
2	15.978286846766851	11.52012328297583
3	6.645822221204435	7.187318519116505
4	3.2598609770962246	4.700629135232914
5	1.875120280653849	3.3798393111073093
6	1.1791985968815697	2.5505575174856583
7	0.8250570565761176	2.0819914452901767
8	0.5873209861923178	1.693801152151308
9	0.45719944683085795	1.4833550801220703
>10	2.217963471085972	14.177758330666675
>50	0.13251599450129697	3.2774226020040076
>100	0.11147994616526082	8.715831827294577
>500	0.02243015437457514	5.616690994752169
>1k	0.014998476720348437	9.292811061728887
>5k	1.3512141189503095E-4	0.279646017629932
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5741	0.2779608201611408	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	4245	0.2055292948239057	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3957	0.19158525786058772	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3457	0.16737686035482732	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3404	0.16481077021921672	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3359	0.1626320144436983	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3283	0.15895233802282271	No Hit
GAACTACGACGGTATCTGATCGTCT	3251	0.15740300058245404	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2943	0.14249062771890564	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2942	0.14244221092389414	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2853	0.13813311616786877	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2845	0.1377457818077766	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2788	0.13498602449211994	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2707	0.13106426409618674	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2705	0.1309674305061637	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2622	0.12694883652020747	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2608	0.12627100139004618	No Hit
TCGTAGTTCCGACCATAAACGATGC	2540	0.12297865932926277	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	2460	0.11910531572834113	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2394	0.11590980725758075	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	2361	0.11431205302220054	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2340	0.11329530032695861	No Hit
CTTTAATATACGCTATTGGAGCTGG	2289	0.11082604378137106	No Hit
GCTTTGAACACTCTAATTTTTTCAA	2217	0.10734003454054156	No Hit
GTACATGGGGAATAATTGCAATCCC	2196	0.10632328184529963	No Hit
GAATAATGGAATAGGACCGCGGTTC	2183	0.10569386351014987	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	2170	0.10506444517500008	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2088	0.10109426798405538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.841679501152078E-5
2	0.0	0.0	0.0	0.0	4.841679501152078E-5
3	0.0	0.0	0.0	0.0	4.841679501152078E-5
4	0.0	0.0	0.0	0.0	9.683359002304156E-5
5	0.0	0.0	0.0	0.0	9.683359002304156E-5
6	0.0	0.0	0.0	0.0	2.420839750576039E-4
7	0.0	0.0	0.0	0.0	2.420839750576039E-4
8	0.0	0.0	0.0	0.0	2.420839750576039E-4
9	0.0	0.0	0.0	0.0	2.420839750576039E-4
10	0.0	0.0	0.0	0.0	2.9050077006912464E-4
11	0.0	0.0	0.0	0.0	2.9050077006912464E-4
12	0.0	0.0	0.0	0.0	3.389175650806454E-4
13	0.0	0.0	0.0	0.0	3.389175650806454E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	235	0.0	12.933911	1
CGGTCCA	665	0.0	12.858636	10
TCGCGTA	230	0.0	12.392743	9
AAGACGG	830	0.0	12.360785	5
TAGGGTG	85	3.947447E-6	12.29346	5
CAAGACG	860	0.0	12.260079	4
GTATCAA	3395	0.0	12.170187	1
AGGCCCG	645	0.0	12.078921	10
GCACGCC	315	0.0	12.064307	15
TATACCG	95	1.040984E-6	11.999358	5
CGCAAGA	850	0.0	11.954998	2
GGTCGGC	400	0.0	11.87609	11
AGAATTT	725	0.0	11.793615	17
GAGGTTA	250	0.0	11.779369	5
GGTTCTA	655	0.0	11.748601	13
TCCATTA	550	0.0	11.746817	8
ACGGTAT	615	0.0	11.7411995	9
CGGTCGG	405	0.0	11.729755	10
ACAATAC	235	0.0	11.7202215	3
CGGACCA	820	0.0	11.702578	9
>>END_MODULE