FastQCFastQC Report
Thu 2 Feb 2017
SRR4063917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065399
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT38340.18562999207417064No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC35480.1717827887008757No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT34520.16713477637976973No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA31600.15299707223640566No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA31150.15081831646088723No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA30250.14646080490985036No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG29570.14316846284906692No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG29290.14181279258874435No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC29170.14123179104860611No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA27280.13208101679142867No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26480.12820767319050702No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT26210.12690041972519595No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT22190.1074368681305646No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT21870.10588753069019592No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC21760.10535494594506921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42100.021.2692621
ATACCGT9500.019.4529426
TACCGTC8600.019.4421717
CGCCCCT5900.019.3901395
ACCGTCG8750.018.8574438
CGCAATA9900.018.44518536
ACCGTTA600.007413154518.3336268
TCTAGCG10350.018.06792328
GCGCAAT10300.017.94246935
CGTCGTA9300.017.74221810
CGAATGC10300.017.7271543
ATACGAA10300.017.7271540
TACGAAT10200.017.68527241
CTAGCGG10450.017.68449229
CCGTCGT9400.017.5538989
ATTAGAC1408.3813575E-817.2855723
TAGCGGC10800.017.111830
AACGCAG52150.016.9590446
GCAATAC10900.016.55072237
CCTAATA800.00199006816.4986672
Produced by FastQC (version 0.11.5)