##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063917_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2065399
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201630290321628	32.0	32.0	32.0	32.0	32.0
2	31.135802815823965	32.0	32.0	32.0	32.0	32.0
3	31.311594999319745	32.0	32.0	32.0	32.0	32.0
4	31.44240362273827	32.0	32.0	32.0	32.0	32.0
5	31.329246310277092	32.0	32.0	32.0	32.0	32.0
6	34.91235398099835	36.0	36.0	36.0	36.0	36.0
7	34.94116536320585	36.0	36.0	36.0	36.0	36.0
8	34.85996168294843	36.0	36.0	36.0	32.0	36.0
9	35.03082310004024	36.0	36.0	36.0	36.0	36.0
10	34.8201393532194	36.0	36.0	36.0	32.0	36.0
11	35.03341049356565	36.0	36.0	36.0	36.0	36.0
12	34.89159576430511	36.0	36.0	36.0	32.0	36.0
13	34.969534215906954	36.0	36.0	36.0	36.0	36.0
14	34.89475205517191	36.0	36.0	36.0	32.0	36.0
15	34.836614136057975	36.0	36.0	36.0	32.0	36.0
16	34.85468037894857	36.0	36.0	36.0	32.0	36.0
17	34.80757277407416	36.0	36.0	36.0	32.0	36.0
18	34.813173144753144	36.0	36.0	36.0	32.0	36.0
19	34.82104668395792	36.0	36.0	36.0	32.0	36.0
20	34.811647531542334	36.0	36.0	36.0	32.0	36.0
21	34.80593192889122	36.0	36.0	36.0	32.0	36.0
22	34.78772866647074	36.0	36.0	36.0	32.0	36.0
23	34.72823459292853	36.0	36.0	36.0	32.0	36.0
24	34.68486282795721	36.0	36.0	36.0	32.0	36.0
25	34.653057351146195	36.0	36.0	36.0	32.0	36.0
26	34.575276254128134	36.0	36.0	36.0	32.0	36.0
27	34.58769855122424	36.0	36.0	36.0	32.0	36.0
28	34.558503708000245	36.0	36.0	36.0	32.0	36.0
29	34.5205110489547	36.0	36.0	36.0	32.0	36.0
30	34.50460032177802	36.0	36.0	36.0	32.0	36.0
31	34.50758909053408	36.0	36.0	36.0	32.0	36.0
32	34.45041660231268	36.0	36.0	36.0	32.0	36.0
33	34.420864927309445	36.0	36.0	36.0	32.0	36.0
34	34.41290036453005	36.0	36.0	36.0	32.0	36.0
35	34.36741714312828	36.0	36.0	36.0	32.0	36.0
36	34.34037248977074	36.0	36.0	36.0	32.0	36.0
37	34.33515751678005	36.0	36.0	36.0	32.0	36.0
38	34.28669811498892	36.0	36.0	36.0	32.0	36.0
39	34.2989887184026	36.0	36.0	36.0	32.0	36.0
40	34.26039665943481	36.0	36.0	36.0	32.0	36.0
41	34.2469212970472	36.0	36.0	36.0	32.0	36.0
42	34.156183381516115	36.0	36.0	36.0	32.0	36.0
43	34.16149470392888	36.0	36.0	36.0	32.0	36.0
44	34.06882495827683	36.0	36.0	36.0	32.0	36.0
45	34.0417996716373	36.0	36.0	36.0	32.0	36.0
46	34.031659742258036	36.0	36.0	36.0	32.0	36.0
47	34.001361480275726	36.0	36.0	36.0	32.0	36.0
48	33.94937539913595	36.0	36.0	36.0	32.0	36.0
49	33.9271012525909	36.0	36.0	36.0	32.0	36.0
50	33.23093842884595	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	3.0
18	0.0
19	6.0
20	26.0
21	62.0
22	225.0
23	714.0
24	1752.0
25	4005.0
26	8272.0
27	15426.0
28	25949.0
29	41315.0
30	61135.0
31	89790.0
32	133954.0
33	220916.0
34	439215.0
35	1022633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.043926991712844	17.7523732051161	11.84585848168135	26.3578413214897
2	16.64175610502428	19.814669294743023	35.62476608522656	27.918808515006134
3	18.411547627232384	23.509073813519358	28.084762519887246	29.99461603936101
4	12.564351972669687	14.991050155442121	34.62386686543375	37.820731006454444
5	15.228244034203561	35.89810007654695	31.992075138992515	16.881580750256973
6	36.181065344306525	34.631097735158065	15.528145180735143	13.65969173980027
7	31.66080742752369	29.655286944556476	19.88119486840073	18.802710759519105
8	29.445787472541625	31.67204012396636	18.34851280551603	20.533659597975984
9	27.505074156276265	13.502630039779836	17.776299429447924	41.21599637449597
10	16.388622324823835	25.687017098080833	29.820497749080317	28.103862828015018
11	39.091109800629226	20.36222558557721	21.225739542693468	19.3209250711001
12	25.779983218722595	22.70991969098435	27.316249612060055	24.193847478233
13	29.56058704594576	18.28137225282174	25.197057023557758	26.96098367767475
14	24.478514805129663	18.787749969860545	23.820917895283188	32.91281732972661
15	25.76771210342198	26.573444857346907	21.387287715008537	26.27155532422258
16	27.003646779485074	25.035780271368424	22.45417801241803	25.506394936728473
17	24.864735578936564	25.47178535479101	23.980644902026192	25.68283416424623
18	26.296667313840334	23.742090480688642	25.00824299127865	24.952999214192374
19	26.35988449672605	23.960344650614214	24.496319349897067	25.183451502762665
20	26.885134621520564	22.9270793843079	23.770458070004448	26.41732792416709
21	28.17784844477992	23.02388061580353	23.537195476515677	25.261075462900873
22	26.994277613456397	23.00390675497253	24.271508092633038	25.730307538938035
23	25.0220782616274	22.742594583079143	25.305609621474012	26.929717533819442
24	25.618298168817756	24.046486203312018	24.423316308275396	25.911899319594827
25	25.8426634439777	23.33389497984851	24.490741547704125	26.33270002846967
26	25.58470364992202	24.639745661235757	24.73140406831626	25.044146620525964
27	26.244581172379366	23.536244711906967	24.10615067500205	26.113023440711626
28	25.282595159495713	23.553663787051125	25.16924721665677	25.994493836796394
29	25.168664233498887	23.86907568998124	25.11264456415535	25.849615512364515
30	24.992930891230625	24.20094861629459	25.308861950957816	25.49725854151697
31	26.087103887262515	23.93236099315437	23.770541017151366	26.209994102431754
32	25.846946566918326	23.758419352636455	23.517247356477	26.877386723968215
33	25.04327439622836	23.575164781638	24.629534854106577	26.75202596802706
34	26.314023480925137	23.614220640128288	24.4633802926013	25.60837558634527
35	26.874303081186106	23.30181795645368	24.70526658793875	25.118612374421463
36	25.270307305390844	24.47311259406888	24.02904841175998	26.2275316887803
37	26.575713764175784	24.048015850343628	24.14674156715818	25.22952881832241
38	25.3744843214348	23.57624295481397	24.688317096318105	26.360955627433132
39	26.429439167037476	23.688727513590017	23.763389681804558	26.11844363756795
40	26.399264435667895	24.062133688526853	24.38246633868399	25.156135537121255
41	25.060317033007205	24.68938483162796	24.990788428232293	25.259509707132537
42	27.166886160281983	24.43176976410892	23.93185691598559	24.469487159623505
43	26.117838450253466	23.266969114492802	24.456005461491163	26.159186973762573
44	25.56485009230787	24.265228862030114	23.717482234426647	26.452438811235368
45	25.638286952665183	24.600895141407943	23.878700786105362	25.882117119821512
46	25.418144404769112	24.233081159595717	24.25879077649337	26.0899836591418
47	25.769266524123825	23.762675854482353	24.777954665179262	25.690102956214556
48	27.06520386587668	24.746278239119416	23.22009845290369	24.968419442100213
49	25.653144778327796	24.91420514846576	23.383745071153562	26.04890500205288
50	25.904414221180712	24.852738250289296	23.551276526724024	25.691571001805976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	23.5
2	30.0
3	95.0
4	160.0
5	197.5
6	235.0
7	245.5
8	256.0
9	339.0
10	422.0
11	682.0
12	942.0
13	1744.5
14	2547.0
15	3481.5
16	4416.0
17	4692.5
18	4969.0
19	5003.5
20	5038.0
21	5406.0
22	5774.0
23	6423.5
24	7073.0
25	7263.0
26	7453.0
27	8211.5
28	8970.0
29	10857.5
30	12745.0
31	15387.5
32	18030.0
33	21553.0
34	25076.0
35	29962.5
36	34849.0
37	44675.5
38	54502.0
39	63284.0
40	72066.0
41	82281.0
42	92496.0
43	98437.0
44	104378.0
45	118990.5
46	133603.0
47	145731.5
48	157860.0
49	166462.0
50	175064.0
51	167836.0
52	160608.0
53	160600.5
54	160593.0
55	164065.5
56	167538.0
57	163734.0
58	159930.0
59	146775.0
60	133620.0
61	117724.0
62	101828.0
63	88304.0
64	74780.0
65	63864.5
66	52949.0
67	46045.5
68	39142.0
69	36295.0
70	33448.0
71	25616.0
72	17784.0
73	15500.5
74	13217.0
75	10209.5
76	7202.0
77	6225.0
78	5248.0
79	4326.5
80	3405.0
81	2798.5
82	2192.0
83	1843.5
84	1495.0
85	1071.0
86	647.0
87	470.5
88	294.0
89	243.5
90	193.0
91	158.0
92	123.0
93	95.5
94	68.0
95	60.5
96	53.0
97	42.5
98	32.0
99	35.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007456186431774199
2	4.841679501152078E-4
3	2.420839750576039E-4
4	0.0
5	0.0
6	4.841679501152077E-5
7	0.0
8	0.0
9	0.001500920645357144
10	0.0010167526952419364
11	4.841679501152077E-5
12	9.683359002304155E-5
13	3.389175650806454E-4
14	0.0
15	0.0035828428308525375
16	7.262519251728116E-4
17	0.0
18	3.873343600921662E-4
19	1.4525038503456235E-4
20	7.746687201843324E-4
21	0.0
22	9.683359002304155E-5
23	9.199191052188947E-4
24	5.810015401382494E-4
25	0.0020819221854953933
26	0.005906848991405534
27	0.007068852071682033
28	0.0037280932158870995
29	0.002566090135610601
30	0.003631259625864058
31	0.006826768096624429
32	0.0032923420607834128
33	0.00435751155103687
34	0.003050258085725809
35	0.0028081741106682047
36	0.007601436816808762
37	0.003921760395933183
38	0.0076982704068318045
39	0.004648012321105995
40	0.0022271725705299557
41	0.003001841290714288
42	0.0018882550054493104
43	0.0014040870553341024
44	0.0013556702603225816
45	0.001307253465311061
46	0.0011620030802764988
47	0.0026629237256336428
48	0.0022271725705299557
49	3.389175650806454E-4
50	0.0014040870553341024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2065399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.70868755164379
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06514885085706	27.424864164264534
2	15.148065117531583	12.030195695280318
3	6.105880974941087	7.273695595843856
4	2.9834186081103966	4.73870549380863
5	1.7753086099464854	3.5247587450054025
6	1.0689285601280967	2.546745012549304
7	0.7427670088955728	2.0646012155932323
8	0.5263490117835482	1.6720502761623512
9	0.38837805120082447	1.387978441834487
>10	1.9598729514340347	13.661361536895953
>50	0.11211214416140709	3.0658280887799028
>100	0.09430248878698623	8.167420864421603
>500	0.017926136852341776	4.901147658832379
>1k	0.0115414853706858	7.54064721072808
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3834	0.18562999207417064	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3548	0.1717827887008757	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3452	0.16713477637976973	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3160	0.15299707223640566	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	3115	0.15081831646088723	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3025	0.14646080490985036	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2957	0.14316846284906692	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2929	0.14181279258874435	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2917	0.14123179104860611	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2728	0.13208101679142867	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2648	0.12820767319050702	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2621	0.12690041972519595	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	2219	0.1074368681305646	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	2187	0.10588753069019592	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	2176	0.10535494594506921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	4.841679501152078E-5
5	0.0	0.0	0.0	0.0	4.841679501152078E-5
6	0.0	0.0	0.0	0.0	1.4525038503456232E-4
7	0.0	0.0	0.0	0.0	1.4525038503456232E-4
8	0.0	0.0	0.0	0.0	1.4525038503456232E-4
9	0.0	0.0	0.0	0.0	1.4525038503456232E-4
10	0.0	0.0	0.0	0.0	1.4525038503456232E-4
11	0.0	0.0	0.0	0.0	1.4525038503456232E-4
12	0.0	0.0	0.0	0.0	2.420839750576039E-4
13	0.0	0.0	0.0	0.0	3.8733436009216624E-4
14	0.0	0.0	0.0	0.0	4.841679501152078E-4
15	0.0	0.0	0.0	0.0	4.841679501152078E-4
16	0.0	0.0	0.0	0.0	5.325847451267286E-4
17	0.0	0.0	0.0	0.0	5.325847451267286E-4
18	0.0	0.0	0.0	0.0	5.810015401382493E-4
19	0.0	0.0	0.0	0.0	5.810015401382493E-4
20	0.0	0.0	0.0	0.0	7.262519251728116E-4
21	0.0	0.0	0.0	0.0	7.262519251728116E-4
22	0.0	0.0	0.0	0.0	7.746687201843325E-4
23	0.0	0.0	0.0	0.0	8.71502310207374E-4
24	0.0	0.0	0.0	1.4525038503456232E-4	9.199191052188947E-4
25	0.0	0.0	0.0	1.9366718004608312E-4	9.199191052188947E-4
26	0.0	0.0	0.0	2.420839750576039E-4	9.683359002304156E-4
27	0.0	0.0	0.0	5.325847451267286E-4	0.0011135862852649778
28	0.0	0.0	0.0	0.0014040870553341026	0.0011135862852649778
29	0.0	0.0	0.0	0.0038249268059101413	0.0011135862852649778
30	4.841679501152078E-5	0.0	0.0	0.007068852071682033	0.0011135862852649778
31	4.841679501152078E-5	0.0	0.0	0.016945878254032272	0.0011135862852649778
32	4.841679501152078E-5	0.0	0.0	0.02817857469670509	0.0011620030802764986
33	4.841679501152078E-5	0.0	0.0	0.038491352034159015	0.0011620030802764986
34	4.841679501152078E-5	0.0	0.0	0.05045030040200465	0.0011620030802764986
35	4.841679501152078E-5	0.0	0.0	0.0647816717254148	0.0011620030802764986
36	4.841679501152078E-5	0.0	0.0	0.08555247678535721	0.0011620030802764986
37	4.841679501152078E-5	0.0	0.0	0.11784647905804158	0.0011620030802764986
38	4.841679501152078E-5	0.0	0.0	0.16369718393395175	0.0011620030802764986
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4210	0.0	21.269262	1
ATACCGT	950	0.0	19.452942	6
TACCGTC	860	0.0	19.442171	7
CGCCCCT	590	0.0	19.390139	5
ACCGTCG	875	0.0	18.857443	8
CGCAATA	990	0.0	18.445185	36
ACCGTTA	60	0.0074131545	18.333626	8
TCTAGCG	1035	0.0	18.067923	28
GCGCAAT	1030	0.0	17.942469	35
CGTCGTA	930	0.0	17.742218	10
CGAATGC	1030	0.0	17.72715	43
ATACGAA	1030	0.0	17.72715	40
TACGAAT	1020	0.0	17.685272	41
CTAGCGG	1045	0.0	17.684492	29
CCGTCGT	940	0.0	17.553898	9
ATTAGAC	140	8.3813575E-8	17.285572	3
TAGCGGC	1080	0.0	17.1118	30
AACGCAG	5215	0.0	16.959044	6
GCAATAC	1090	0.0	16.550722	37
CCTAATA	80	0.001990068	16.498667	2
>>END_MODULE