FastQCFastQC Report
Thu 2 Feb 2017
SRR4063916_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063916_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569200
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39710.25305888350751976No Hit
GTCCTAAAGTGTGTATTTCTCATTT38390.24464695386184043No Hit
GTCCTACAGTGGACATTTCTAAATT34910.2224700484323222No Hit
CTGTAGGACGTGGAATATGGCAAGA33800.21539638032118277No Hit
CTTTAGGACGTGAAATATGGCGAGG32090.20449910782564365No Hit
TATCAACGCAGAGTACTTTTTTTTT28020.17856232475146572No Hit
GTCCTACAGTGTGCATTTCTCATTT23700.15103237318378793No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18980.12095335202651032No Hit
CTGTAGGACCTGGAATATGGCGAGA17470.1113306143257711No Hit
ATTTAGAAATGTCCACTGTAGGACG17410.11094825388733112No Hit
GATATACACTGTTCTACAAATCCCG15890.1012617894468519No Hit
CTGAAGGACCTGGAATATGGCGAGA15820.10081570226867194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATA250.00603034619.00095711
ACAGTCG500.001497907913.3019438
ACCGAGC500.001497907913.3019438
CCGTCGT2100.012.66892059
ACTATTC604.087023E-412.6685178
TAGAAAT5100.012.6644774
GTCTAGT604.0991357E-412.6640741
GCGTTAT604.0991357E-412.6640741
CGCATCG1750.012.48634213
ATTTAGA5050.012.4132991
CACCTTT5000.012.16114
TTTGCGC1501.4551915E-1112.03432317
CGTCGTA2150.011.93235610
GTATCAA26150.011.913431
AAATGTC4950.011.9003497
GTGACTA802.8649216E-511.87559812
ATTTGCG1603.6379788E-1211.87559816
TAGGACC9150.011.8340214
GTCCTAA11050.011.7758691
TTTAGAA5750.011.725792