##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063916_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569200
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.284398419576853	32.0	32.0	32.0	32.0	32.0
2	31.00286005607953	32.0	32.0	32.0	32.0	32.0
3	31.009486362477695	32.0	32.0	32.0	32.0	32.0
4	31.029005862860057	32.0	32.0	32.0	32.0	32.0
5	30.99496303849095	32.0	32.0	32.0	32.0	32.0
6	34.625312261024725	36.0	36.0	36.0	32.0	36.0
7	34.57051045118532	36.0	36.0	36.0	32.0	36.0
8	34.54515230690798	36.0	36.0	36.0	32.0	36.0
9	34.616314682640834	36.0	36.0	36.0	32.0	36.0
10	34.41394978332909	36.0	36.0	36.0	32.0	36.0
11	34.59351261789447	36.0	36.0	36.0	32.0	36.0
12	34.4599509304104	36.0	36.0	36.0	32.0	36.0
13	34.51244774407341	36.0	36.0	36.0	32.0	36.0
14	34.45208768799388	36.0	36.0	36.0	32.0	36.0
15	34.409316849349985	36.0	36.0	36.0	32.0	36.0
16	34.40799961763956	36.0	36.0	36.0	32.0	36.0
17	34.35295883252613	36.0	36.0	36.0	32.0	36.0
18	34.3472310731583	36.0	36.0	36.0	32.0	36.0
19	34.33204435381086	36.0	36.0	36.0	32.0	36.0
20	34.3027192199847	36.0	36.0	36.0	32.0	36.0
21	34.28466734641856	36.0	36.0	36.0	32.0	36.0
22	34.27368213102218	36.0	36.0	36.0	32.0	36.0
23	34.23920341575325	36.0	36.0	36.0	32.0	36.0
24	34.22310859036452	36.0	36.0	36.0	32.0	36.0
25	33.84159508029569	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	15.0
5	20.0
6	46.0
7	24.0
8	72.0
9	98.0
10	157.0
11	44.0
12	90.0
13	89.0
14	141.0
15	248.0
16	451.0
17	591.0
18	776.0
19	904.0
20	1414.0
21	1953.0
22	3013.0
23	4600.0
24	6919.0
25	9846.0
26	14542.0
27	19615.0
28	27163.0
29	37620.0
30	49817.0
31	68760.0
32	99420.0
33	142497.0
34	318686.0
35	759566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5564286055815	17.63855728925936	11.742696396410755	26.062317708748385
2	15.340097621410177	19.831332534160804	40.37401784308815	24.454552001340875
3	18.96169907958238	24.254785464245955	28.952210041046943	27.831305415124724
4	12.238170013281092	15.974528852600264	36.76026735527815	35.0270337788405
5	13.72125141086129	37.342781775527904	34.36345061459699	14.572516199013815
6	32.968626316265805	36.303315058679864	17.55399223210382	13.174066392950511
7	29.21749162611187	30.606967457365673	21.300303085351512	18.875237831170942
8	26.462100237720183	35.648592325507714	19.69998425398639	18.189323182785717
9	27.40106996876548	14.18158100604607	18.907435779240362	39.509913245948084
10	15.871333775840268	27.112093893085476	32.557352150717605	24.459220180356645
11	36.36561875340723	20.56568825297844	24.40384989367841	18.664843099935922
12	25.335493756898785	23.11602201489468	30.497936821001765	21.05054740720477
13	30.0497127932009	20.26121534898432	25.515174007529613	24.17389785028517
14	22.501323198377726	21.117098273784094	25.62037279121524	30.761205736622944
15	23.7654356412032	30.340256815402295	22.282861671914432	23.611445871480075
16	23.60578379326287	26.010234827107944	26.953751972834667	23.430229406794524
17	22.483519762893735	27.036324750899414	26.90031257930855	23.579842906898296
18	23.07754612135463	25.34109107050268	29.322830668444748	22.25853213969794
19	25.435030770702465	24.51037913331845	25.930423137017318	24.12416695896177
20	25.466034593928555	25.419668703917118	26.213054772338378	22.901241929815946
21	25.53508326068365	24.502639304042596	24.9521851345672	25.010092300706553
22	25.298773655239533	26.272502492039184	25.442843175958057	22.985880676763227
23	23.342923674195433	25.978492209628566	26.661317675842227	24.017266440333774
24	24.74753852453057	25.236006995067484	26.722267503931857	23.294186976470094
25	24.034908474948118	25.712474473624127	26.823581332996593	23.42903571843116
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	137.0
1	137.0
2	364.5
3	592.0
4	592.0
5	592.0
6	1591.0
7	2590.0
8	2590.0
9	2590.0
10	2796.5
11	3003.0
12	3003.0
13	3003.0
14	3409.5
15	3816.0
16	3816.0
17	3816.0
18	6981.5
19	10147.0
20	10147.0
21	10147.0
22	18308.5
23	26470.0
24	26470.0
25	26470.0
26	42799.5
27	59129.0
28	59129.0
29	59129.0
30	79671.0
31	100213.0
32	100213.0
33	100213.0
34	112443.5
35	124674.0
36	124674.0
37	124674.0
38	135993.0
39	147312.0
40	147312.0
41	147312.0
42	161833.5
43	176355.0
44	176355.0
45	176355.0
46	189109.0
47	201863.0
48	201863.0
49	201863.0
50	200255.5
51	198648.0
52	198648.0
53	198648.0
54	179592.5
55	160537.0
56	160537.0
57	160537.0
58	145657.0
59	130777.0
60	130777.0
61	130777.0
62	113885.5
63	96994.0
64	96994.0
65	96994.0
66	78994.5
67	60995.0
68	60995.0
69	60995.0
70	46074.0
71	31153.0
72	31153.0
73	31153.0
74	23847.5
75	16542.0
76	16542.0
77	16542.0
78	13218.0
79	9894.0
80	9894.0
81	9894.0
82	6695.5
83	3497.0
84	3497.0
85	3497.0
86	2731.0
87	1965.0
88	1965.0
89	1965.0
90	1404.5
91	844.0
92	844.0
93	844.0
94	578.0
95	312.0
96	312.0
97	312.0
98	526.5
99	741.0
100	741.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03982921233749681
2	0.004906958959979607
3	0.001338261534539893
4	0.003313790466479735
5	0.007838388988019373
6	0.011343359673719092
7	0.02134845781289829
8	0.034985980117257204
9	0.047348967626816216
10	0.054996176395615604
11	0.05384909508029569
12	0.06302574560285497
13	0.06321692582207494
14	0.06563854193219475
15	0.05824624012235534
16	0.06531990823349478
17	0.06105021667091511
18	0.07755544226357379
19	0.07328575070099413
20	0.07882997705837369
21	0.07475146571501402
22	0.07691817486617385
23	0.08303594188121335
24	0.07889370379811368
25	0.0773642620443538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1569200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.40891771580458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95578879878296	37.03484401082294
2	14.293696400939304	14.124721386574043
3	4.637965647283572	6.87470589106087
4	2.01733253380961	3.9869686867365832
5	1.0571977921241702	2.6117499360196703
6	0.6579556584117499	1.9505326192268504
7	0.4492092633831073	1.5536460472171199
8	0.30257569166896425	1.1959949961979608
9	0.22913930066959673	1.0189372367016998
>10	1.2016823124331617	10.62970085064946
>50	0.09408466333918047	3.225377283895729
>100	0.0896409525360089	9.034539433590265
>500	0.009715319305659801	3.2528306501346824
>1k	0.0040156653130060515	3.5054509711720394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3971	0.25305888350751976	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3839	0.24464695386184043	No Hit
GTCCTACAGTGGACATTTCTAAATT	3491	0.2224700484323222	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3380	0.21539638032118277	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3209	0.20449910782564365	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2802	0.17856232475146572	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2370	0.15103237318378793	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1898	0.12095335202651032	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1747	0.1113306143257711	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1741	0.11094825388733112	No Hit
GATATACACTGTTCTACAAATCCCG	1589	0.1012617894468519	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1582	0.10081570226867194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATA	25	0.006030346	19.000957	11
ACAGTCG	50	0.0014979079	13.301943	8
ACCGAGC	50	0.0014979079	13.301943	8
CCGTCGT	210	0.0	12.6689205	9
ACTATTC	60	4.087023E-4	12.668517	8
TAGAAAT	510	0.0	12.664477	4
GTCTAGT	60	4.0991357E-4	12.664074	1
GCGTTAT	60	4.0991357E-4	12.664074	1
CGCATCG	175	0.0	12.486342	13
ATTTAGA	505	0.0	12.413299	1
CACCTTT	500	0.0	12.161	14
TTTGCGC	150	1.4551915E-11	12.034323	17
CGTCGTA	215	0.0	11.932356	10
GTATCAA	2615	0.0	11.91343	1
AAATGTC	495	0.0	11.900349	7
GTGACTA	80	2.8649216E-5	11.875598	12
ATTTGCG	160	3.6379788E-12	11.875598	16
TAGGACC	915	0.0	11.834021	4
GTCCTAA	1105	0.0	11.775869	1
TTTAGAA	575	0.0	11.72579	2
>>END_MODULE