FastQCFastQC Report
Thu 2 Feb 2017
SRR4063916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569200
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA18010.11477185827173082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG703.594323E-828.2840355
TTAATAC1950.021.4346263
GTCCTAC19550.020.7079521
TTAGGAC15050.020.317733
TGTAGGA24950.020.1042962
TAGGACC12550.019.8075894
GTAGGAC24550.019.7136973
TAGGACG26350.019.7028164
TAATACT1900.019.6830434
GTATCAA31250.019.6435621
GACGTGA15050.019.1483657
AGGACGT26850.018.926255
CTGTAGG25150.018.896521
TCCTACA21200.018.78312
GGACGTG26450.018.7134446
GTCCTAA12800.018.5643561
TTTAGGA17600.018.3750782
TAGGACA7200.018.3322434
AGGACCT18550.018.1444745
GTATAGG1951.8189894E-1218.0530851
Produced by FastQC (version 0.11.5)