##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569200
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.337913586540914	32.0	32.0	32.0	32.0	32.0
2	31.415660846291104	32.0	32.0	32.0	32.0	32.0
3	31.479645042059648	32.0	32.0	32.0	32.0	32.0
4	31.559267779760386	32.0	32.0	32.0	32.0	32.0
5	31.50760068824879	32.0	32.0	32.0	32.0	32.0
6	35.10331442773388	36.0	36.0	36.0	36.0	36.0
7	35.12228332908489	36.0	36.0	36.0	36.0	36.0
8	35.066579785878154	36.0	36.0	36.0	36.0	36.0
9	35.18393257710935	36.0	36.0	36.0	36.0	36.0
10	35.03528039765486	36.0	36.0	36.0	36.0	36.0
11	35.178525363242414	36.0	36.0	36.0	36.0	36.0
12	35.0845341575325	36.0	36.0	36.0	36.0	36.0
13	35.12259622737701	36.0	36.0	36.0	36.0	36.0
14	35.0719844506755	36.0	36.0	36.0	36.0	36.0
15	35.03947552893194	36.0	36.0	36.0	36.0	36.0
16	35.06043525363243	36.0	36.0	36.0	36.0	36.0
17	35.03319844506755	36.0	36.0	36.0	36.0	36.0
18	35.0305149120571	36.0	36.0	36.0	36.0	36.0
19	35.00945832271221	36.0	36.0	36.0	36.0	36.0
20	35.01023515166964	36.0	36.0	36.0	36.0	36.0
21	34.999314300280396	36.0	36.0	36.0	36.0	36.0
22	34.98212974764211	36.0	36.0	36.0	36.0	36.0
23	34.94329339790976	36.0	36.0	36.0	36.0	36.0
24	34.91001529441754	36.0	36.0	36.0	32.0	36.0
25	34.8853428498598	36.0	36.0	36.0	32.0	36.0
26	34.82969156257966	36.0	36.0	36.0	32.0	36.0
27	34.81442645934234	36.0	36.0	36.0	32.0	36.0
28	34.78035049706857	36.0	36.0	36.0	32.0	36.0
29	34.755764083609485	36.0	36.0	36.0	32.0	36.0
30	34.7286024725975	36.0	36.0	36.0	32.0	36.0
31	34.71802956920724	36.0	36.0	36.0	32.0	36.0
32	34.68013000254907	36.0	36.0	36.0	32.0	36.0
33	34.6700503441244	36.0	36.0	36.0	32.0	36.0
34	34.66493244965588	36.0	36.0	36.0	32.0	36.0
35	34.6239943920469	36.0	36.0	36.0	32.0	36.0
36	34.592629365281674	36.0	36.0	36.0	32.0	36.0
37	34.55903900076472	36.0	36.0	36.0	32.0	36.0
38	34.51820481774153	36.0	36.0	36.0	32.0	36.0
39	34.468566785623246	36.0	36.0	36.0	32.0	36.0
40	34.47416263063982	36.0	36.0	36.0	32.0	36.0
41	34.45901096099924	36.0	36.0	36.0	32.0	36.0
42	34.41555187356615	36.0	36.0	36.0	32.0	36.0
43	34.40580295692072	36.0	36.0	36.0	32.0	36.0
44	34.324974509304106	36.0	36.0	36.0	32.0	36.0
45	34.29552319653327	36.0	36.0	36.0	32.0	36.0
46	34.233644532245734	36.0	36.0	36.0	32.0	36.0
47	34.21331697680347	36.0	36.0	36.0	32.0	36.0
48	34.16221514147336	36.0	36.0	36.0	32.0	36.0
49	34.134277976038746	36.0	36.0	36.0	32.0	36.0
50	33.556154091256694	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	5.0
20	16.0
21	34.0
22	140.0
23	419.0
24	1080.0
25	2533.0
26	5462.0
27	10101.0
28	17024.0
29	27215.0
30	39905.0
31	57534.0
32	84291.0
33	135605.0
34	277997.0
35	909838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.762309249335914	18.52040452142169	12.267695900230965	26.449590329011436
2	14.704956436178621	20.631700900845786	39.79525768675853	24.86808497621706
3	18.31758536372425	24.866986108181447	29.29562062634583	27.51980790174847
4	11.969219984705584	16.651351006882486	36.821246495029314	34.55818251338261
5	13.379492735151668	37.83106041294928	34.07953097119552	14.709915880703543
6	33.0655527197066	36.609854097164465	17.267324965985743	13.057268217143184
7	29.251776545583507	30.797853934209662	21.271771485670694	18.678598034536133
8	26.568824878919195	35.866619933724195	19.48030843742034	18.084246749936273
9	27.50036802684716	14.037404273189994	18.79912999090607	39.66309770905677
10	15.776822152603767	27.426796534258486	32.44276657736358	24.35361473577417
11	36.58736936018353	20.50108335457558	24.341256691307674	18.570290593933215
12	25.387108573933947	23.19127350404474	30.40419373463387	21.017424187387444
13	30.169373576498508	20.285828265976036	25.50130831496934	24.043489842556117
14	22.53163088821395	21.168064624259333	25.611492269279644	30.688812218247076
15	23.867651332459815	30.34793847283332	22.18748247434244	23.596927720364434
16	23.782221262065946	26.064100627776515	26.65306728507528	23.500610825082255
17	22.633443793015548	27.218773897527406	26.61311496303849	23.53466734641856
18	23.28767297882035	25.435016043257846	29.133791530052033	22.143519447869767
19	25.633507519755288	24.599286260514912	25.835202651032375	23.932003568697425
20	25.739632715201655	25.47503777424163	26.030037133784223	22.75529237677249
21	25.64618914096355	24.50274024980882	24.967626816212082	24.88344379301555
22	25.445721033469944	26.213373829578018	25.45706440037242	22.883840736579618
23	23.45435589452862	25.95202347207211	26.501799661480106	24.091820971919162
24	24.938169012558653	25.29421098816783	26.577096438496888	23.190523560776626
25	24.301940323903512	25.684708677384428	26.72500320232785	23.28834779638421
26	22.729882768312073	26.931614317624902	27.374539151050115	22.96396376301291
27	23.63057000101968	26.359997960640356	26.4062659324972	23.603166105842767
28	22.9261490733105	26.620224913869688	26.136391155885985	24.317234856933826
29	23.810209646798963	25.36677196696583	25.675152931849652	25.147865454385553
30	22.8650834720498	26.13195186177467	26.691236263246044	24.31172840292949
31	24.77112573951958	25.071235420717574	25.852578527850167	24.30506031191268
32	23.672834962556234	26.49015105601557	25.74401603922626	24.09299794220194
33	22.95894046150238	26.30033343190138	27.089047730725696	23.651678375870546
34	23.9535722570465	26.136157965978988	26.914669616887636	22.995600160086877
35	23.93655224275331	26.455662725079897	26.708918437512946	22.898866594653843
36	22.873046555098604	26.484063662499274	26.864922085263597	23.77796769713853
37	23.763323338506	26.2816374522431	25.542317631576434	24.412721577674464
38	23.86691275629734	27.016664584761983	25.506795081946315	23.609627576994356
39	24.9445238225731	25.31428003329208	25.716346895309666	24.024849248825152
40	25.63511687781998	25.556348619643632	26.211667388921462	22.596867113614927
41	24.435873643853494	25.199215882208993	27.23898003507627	23.125930438861246
42	24.543549663708422	27.033713278450954	26.220801517231358	22.201935540609263
43	23.223770871556564	26.47075130274965	26.867646243486256	23.437831582207533
44	22.917805792302506	26.844809984284844	25.886861085938822	24.35052313747382
45	23.12201755817036	27.085160185166302	25.974646695352487	23.81817556131085
46	23.55038175771051	25.498394393537044	26.022869138134414	24.928354710618034
47	24.78918764593721	25.56246367501759	26.0278695461544	23.620479132890807
48	23.903850051045964	26.294662032381368	25.286870672086074	24.51461724448659
49	24.26801547037235	26.08863281954482	25.559124976978616	24.084226733104213
50	23.610997817974184	27.530104818810287	24.888604523115198	23.97029284010033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	32.0
2	36.0
3	81.5
4	127.0
5	161.5
6	196.0
7	216.0
8	236.0
9	300.0
10	364.0
11	593.5
12	823.0
13	1496.0
14	2169.0
15	2907.5
16	3646.0
17	3973.5
18	4301.0
19	4332.0
20	4363.0
21	4752.5
22	5142.0
23	6155.0
24	7168.0
25	8817.5
26	10467.0
27	14928.5
28	19390.0
29	24655.5
30	29921.0
31	35797.0
32	41673.0
33	51598.0
34	61523.0
35	73045.0
36	84567.0
37	87426.5
38	90286.0
39	88093.5
40	85901.0
41	84883.0
42	83865.0
43	82662.0
44	81459.0
45	87049.5
46	92640.0
47	97800.5
48	102961.0
49	105746.0
50	108531.0
51	105284.0
52	102037.0
53	100592.5
54	99148.0
55	98380.5
56	97613.0
57	94432.0
58	91251.0
59	82533.0
60	73815.0
61	64712.0
62	55609.0
63	48022.5
64	40436.0
65	34353.0
66	28270.0
67	23965.0
68	19660.0
69	17581.0
70	15502.0
71	11921.0
72	8340.0
73	7227.5
74	6115.0
75	4683.0
76	3251.0
77	2884.5
78	2518.0
79	2085.5
80	1653.0
81	1300.0
82	947.0
83	801.0
84	655.0
85	476.0
86	297.0
87	197.0
88	97.0
89	78.5
90	60.0
91	56.5
92	53.0
93	35.5
94	18.0
95	28.5
96	39.0
97	26.0
98	13.0
99	16.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008666836604639306
2	5.098139179199591E-4
3	1.9118021921998472E-4
4	0.0
5	0.0
6	3.186336986999745E-4
7	1.9118021921998472E-4
8	0.0
9	0.001338261534539893
10	7.647208768799389E-4
11	0.0
12	1.2745347947998978E-4
13	3.8236043843996943E-4
14	3.8236043843996943E-4
15	0.004588325261279633
16	7.00994137139944E-4
17	0.0
18	3.186336986999745E-4
19	0.0
20	5.735406576599542E-4
21	0.0
22	6.372673973999489E-5
23	0.0010196278358399183
24	1.9118021921998472E-4
25	0.0018480754524598522
26	0.005416772877899567
27	0.005098139179199592
28	0.004205964822839664
29	0.002357889370379811
30	0.003313790466479735
31	0.006818761152179455
32	0.0029951567677797605
33	0.004078511343359673
34	0.003313790466479735
35	0.0022304358908998216
36	0.007201121590619424
37	0.0035049706856997193
38	0.007902115727759368
39	0.0036961509049197043
40	0.002549069589599796
41	0.0030588835075197555
42	0.0016568952332398675
43	0.001210808055059903
44	8.921743563599286E-4
45	0.0010196278358399183
46	7.00994137139944E-4
47	0.0020392556716798366
48	0.0016568952332398675
49	4.460871781799643E-4
50	0.0010196278358399183
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1569200.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.28176413517767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59262492915055	42.3417542582743
2	13.621980849026544	15.060942646995851
3	4.354431409625	7.2216195038909525
4	1.8422590730093589	4.073733261999777
5	0.9948634700299317	2.749890384844954
6	0.584573416074682	1.938974982428139
7	0.3869240996850018	1.497289277190157
8	0.27882633009083835	1.2331209131807135
9	0.2063523600951816	1.026677024956717
>10	0.9940541910721403	9.701365840266956
>50	0.07190641972172042	2.7619633262868546
>100	0.06285403934482438	6.896081355654688
>500	0.0068418801580199225	2.4811967056974704
>1k	0.0015075329161738811	1.0153905183325298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1801	0.11477185827173082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	1.274534794799898E-4
19	0.0	0.0	0.0	0.0	1.274534794799898E-4
20	0.0	0.0	0.0	0.0	1.274534794799898E-4
21	0.0	0.0	0.0	6.37267397399949E-5	1.274534794799898E-4
22	0.0	0.0	0.0	6.37267397399949E-5	1.274534794799898E-4
23	0.0	0.0	0.0	1.274534794799898E-4	1.274534794799898E-4
24	0.0	0.0	0.0	1.9118021921998472E-4	1.274534794799898E-4
25	0.0	0.0	0.0	3.1863369869997453E-4	1.274534794799898E-4
26	0.0	0.0	0.0	3.1863369869997453E-4	1.274534794799898E-4
27	0.0	0.0	0.0	4.460871781799643E-4	1.9118021921998472E-4
28	0.0	0.0	0.0	6.372673973999491E-4	1.9118021921998472E-4
29	0.0	0.0	0.0	0.001975528931939842	1.9118021921998472E-4
30	0.0	0.0	0.0	0.004205964822839663	1.9118021921998472E-4
31	0.0	0.0	0.0	0.010897272495539128	1.9118021921998472E-4
32	0.0	0.0	0.0	0.020073923018098395	1.9118021921998472E-4
33	0.0	0.0	0.0	0.028485852663777723	1.9118021921998472E-4
34	0.0	0.0	0.0	0.03785368340555697	3.1863369869997453E-4
35	0.0	0.0	0.0	0.05136375223043589	3.1863369869997453E-4
36	0.0	0.0	0.0	0.06939841957685444	3.1863369869997453E-4
37	0.0	0.0	0.0	0.1025363242416518	3.1863369869997453E-4
38	0.0	0.0	0.0	0.14918429773132807	3.1863369869997453E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	70	3.594323E-8	28.284035	5
TTAATAC	195	0.0	21.434626	3
GTCCTAC	1955	0.0	20.707952	1
TTAGGAC	1505	0.0	20.31773	3
TGTAGGA	2495	0.0	20.104296	2
TAGGACC	1255	0.0	19.807589	4
GTAGGAC	2455	0.0	19.713697	3
TAGGACG	2635	0.0	19.702816	4
TAATACT	190	0.0	19.683043	4
GTATCAA	3125	0.0	19.643562	1
GACGTGA	1505	0.0	19.148365	7
AGGACGT	2685	0.0	18.92625	5
CTGTAGG	2515	0.0	18.89652	1
TCCTACA	2120	0.0	18.7831	2
GGACGTG	2645	0.0	18.713444	6
GTCCTAA	1280	0.0	18.564356	1
TTTAGGA	1760	0.0	18.375078	2
TAGGACA	720	0.0	18.332243	4
AGGACCT	1855	0.0	18.144474	5
GTATAGG	195	1.8189894E-12	18.053085	1
>>END_MODULE